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Plasmid#171568PurposeBacterial expression of ALFA-His-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-ALFA-His)DepositorInsertNb127D01-ALFA-His (CXCR2 Human)
UseTagsALFA-tag/His-tag and PelB signal peptideExpressionBacterialMutationPromoterAvailable sinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLX307-VHL-3F-BirA
Plasmid#220147PurposeExpresses VHL-BirA for VHL substrate identification by E-STUBDepositorInsertvon Hippel-Lindau tumor suppressor (VHL Human)
UseLentiviralTags3F-BirAExpressionMammalianMutationcodon optimizedPromoterEF-1aAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-SLC51A_STOP
Plasmid#161406PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.DepositorInsertSLC51A (SLC51A Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 15, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR221-SLC51B_STOP
Plasmid#161323PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.DepositorInsertSLC51B (SLC51B Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 15, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR221-SLCO2A1_STOP
Plasmid#161115PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.DepositorInsertSLCO2A1 (SLCO2A1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 21, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRK5 HA-RagA
Plasmid#99710PurposeMammalian expression of HA-RagADepositorInsertRagA (RRAGA )
UseTagsHAExpressionMutationPromoterCMVAvailable sinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pQE-30-HyPer3
Plasmid#42132PurposeGenetically encoded H2O2 probe for ratiometric and fluorescence lifetime imagingDepositorInsertHYPER 3
UseTags6HisExpressionBacterialMutationPromoterT5Available sinceOct. 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEF6a-FKBP-CD28
Plasmid#113401PurposeExpresses FKBP-CD28 in mammalian cellDepositorInsertFKBP-CD28
UseTagsExpressionMammalianMutationPromoterEF-1αAvailable sinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pX330-EN1201
Plasmid#92144PurposeFor transient expression of spCas9-nuclease and a sgRNA targeting the mouse TIGRE acceptor locusDepositorInsertspCas9-nuclease and sgRNA against mouse TIGRE acceptor locus
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-NIR-GECO2
Plasmid#159603PurposeAAV production plasmid encoding for near-infrared calcium indicator NIR-GECO2DepositorInsertNIR-GECO2
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGWB514
Plasmid#74856PurposeGateway cloning compatible binary vector for C-terminal fusion with 3xHA (CaMV35S promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterCaMV35SAvailable sinceJuly 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-TLR2-CFP
Plasmid#13015DepositorInsertToll-Like Receptor 2 (TLR2 Human)
UseTagsCFPExpressionMammalianMutationPromoterAvailable sinceNov. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
pBiex-1 Myosin VI HMM_SNAP
Plasmid#82716PurposeExpresses Myosin VI HMM with a C-terminal SNAP tag.DepositorInsertMyosin VI
UseTags6xHis Tag, FLAG purification tag, and SNAP tagExpressionBacterial and InsectMutationPromoterT7 PromoterAvailable sinceNov. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTYB2-eIF4B
Plasmid#37226DepositorInsertTIF3
UseTagsExpressionBacterialMutationPromoterT7Available sinceJuly 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pWMD23
Plasmid#140006PurposeExpression of ORF1 (Shuffle 1 NLS) and Pong TPase (L418A, L420A)DepositorInsertsORF1 Shuffle 1 NLS
Pong TPase LA
UseTagsExpressionPlantMutationNES sequence mutated L418A, L420A and Ping N-term…PromoterAvailable sinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pClneoMyc human ERBIN
Plasmid#40214DepositorInsertERBIN (ERBIN Human)
UseTagsmycExpressionMammalianMutationL143S, S274L and P338L mutation in ERBIN compared…PromoterCMVAvailable sinceSept. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pME.A (pDPL6)
Plasmid#24571DepositorTypeEmpty backboneUseTagsExpressionMutationPromoterAvailable sinceMay 3, 2010AvailabilityAcademic Institutions and Nonprofits only -
TFORF3427
Plasmid#144903PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertNFIB (NFIB Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceMay 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSLPB2B-ER50-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin
Plasmid#187948PurposeER50 degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.DepositorInsertER50-SpdCas9-tagRFPt-P2A-tagBFP
UseCRISPR and Synthetic BiologyTagsGGS linkerExpressionMammalianMutation6 mutations, T371A, L384M, M421G, G521R, Y537S, N…PromoterAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-ArchT-BFP2-TSERex
Plasmid#123312PurposeExpresses the proton pump rhodopsin in mammalian cells under CAG promoterDepositorInsertArchT-BFP2
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only