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pMyc-C1-Cep123 (CCDC123/Cep89)

Plasmid

#69836

Purpose

This plasmid encodes Cep123 (CCDC123/Cep89) isoform 1 with an N-ter Myc-tag

Depositor

Michel Bornens

Article

Sillibourne et al Biol Open. 2013 Apr 9;2(6):535-45. doi:

Insert

Cep123 (CEP89 Human)

Use

Tags

Myc tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pRS306-pHIS3-2xGFP-tURA3

Plasmid

#116936

Purpose

Yeast integrative vector for the expression of 2xGFP

Depositor

Liam Holt

Article

Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

Insert

2xGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

HIS3

Available since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-C2-Cep123-C2 domain

Plasmid

#73329

Purpose

Encodes the distal appendage targeting domain of isoform 1 of Cep123 (residues 500-600) fused to EGFP

Depositor

Michel Bornens

Article

Sillibourne et al Biol Open. 2013 Apr 9;2(6):535-45. doi:

Insert

Cep123 (CEP89 Human)

Use

Tags

EGFP tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 4, 2016

Availability

Academic Institutions and Nonprofits only

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BII-ChiNW-IGF2

Plasmid

#133383

Purpose

Piggybac vector for constitutive expression

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Insert

IGF2 (IGF2 Human)

Use

Tags

Expression

Mammalian

Mutation

N/A

Promoter

CMV enhancer and hEf1a (CpG free)

Available since

Jan. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pTargeting_Mettl3C_FKBP-V

Plasmid

#165421

Purpose

Template for knockin FBKP-V into Mettl3 C-terminus

Depositor

Neil Brockdorff

Article

Wei et al Genome Res. 2021 Aug;31(8):1395-1408. do

Insert

Mettl3 C-terminus + linker + FKBP-V (Mettl3 Mouse)

Use

Mouse Targeting

Tags

FKBP-V

Expression

Mutation

Promoter

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pT7-HISx6-SIMx6

Plasmid

#116938

Purpose

Bacterial expression vector for 6 sim

Depositor

Liam Holt

Article

Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

Insert

6 sim

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pT7-HISx6-SUMOx10

Plasmid

#116937

Purpose

Bacterial expression vector for 10 sumo

Depositor

Liam Holt

Article

Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

Insert

10 SUMO

Use

Tags

HIS

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 21, 2018

Availability

Academic Institutions and Nonprofits only

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VRER variant

Plasmid

#138561

Purpose

Expresses human codon-optimized SpCas9-VRER and blasticidin resistance: EFS promoter-VRER-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

Insert

VRER

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Mutation

D1135V, G1218R, R1335E, T1337R

Promoter

EFS

Available since

June 9, 2020

Availability

Academic Institutions and Nonprofits only

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pR2Q306c

Plasmid

#101027

Purpose

fluorescent, functional, glutamate receptor subunit

Depositor

Thomas Hughes

Article

Sheridan et al Neuroscience. 2006 Aug 25;141(2):837-49.

Insert

Glutamate receptor subunit (Gria2 Rat)

Use

Tags

fluorescent protein

Expression

Mammalian

Mutation

tagged with fluorescent protein

Promoter

CMV

Available since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti CMV_mIFP IRES EGFP

Plasmid

#72443

Purpose

Expressing mIFP and EGFP in mammalian cells

Depositor

Xiaokun Shu

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

mIFP T2A HO1 IRES EGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 24, 2016

Availability

Academic Institutions and Nonprofits only
pR2Q144c

Plasmid

#101023

Purpose

fluorescent, functional, glutamate receptor subunit

Depositor

Thomas Hughes

Article

Sheridan et al Neuroscience. 2006 Aug 25;141(2):837-49.

Insert

Glutamate receptor subunit (Gria2 Rat)

Use

Tags

fluorescent protein

Expression

Mammalian

Mutation

tagged with fluorescent protein

Promoter

CMV

Available since

July 27, 2018

Availability

Academic Institutions and Nonprofits only

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pHS18 (mANGPTL3-Strep)

Plasmid

#109111

Purpose

Expresses StrepII-tagged mouse ANGPTL3 in mammalian cells

Depositor

Brandon Davies

Article

Chi et al Mol Metab. 2017 Oct;6(10):1137-1149. doi

Insert

ANGPTL3 (Angptl3 Mouse)

Use

Tags

StrepII

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 5, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pWL1 (mANGPTL8-V5-His)

Plasmid

#109113

Purpose

Expresses V5 and His-tagged mouse ANGPTL8 in mammalian cells

Depositor

Brandon Davies

Article

Chi et al Mol Metab. 2017 Oct;6(10):1137-1149. doi

Insert

ANGPTL8 (Angptl8 Mouse)

Use

Tags

6x His and V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 21, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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mIFP-Paxillin-22

Plasmid

#56234

Purpose

Localization: Focal Adhesions, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

Paxillin

Use

Tags

mIFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pluc-miR148a-148aT

Plasmid

#65053

Purpose

Reporter plasmid to detect miR148/152 family expression

Depositor

Cristina Fillat

Article

Bofill-De Ros et al Mol Ther. 2014 Sep;22(9):1665-77. doi: 1

Insert

Engineered target sites miR-148a-3p (MIR148A Human, Mouse)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

SV40

Available since

June 2, 2015

Availability

Academic Institutions and Nonprofits only

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mIFP12-C1

Plasmid

#54819

Purpose

Localization: C1 Cloning Vector, Excitation: 683, Emission: 704

Depositor

Xiaokun Shu, Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Type

Empty backbone

Use

Tags

mIFP12

Expression

Mammalian

Mutation

Promoter

Available since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
CMV BARK p2A mCherry

Plasmid

#117689

Purpose

CMV-iBARK-p2A-mCherry: Mammalian expression vector for Gaq silencing

Depositor

Baljit Khakh

Article

Nagai et al Neuron. 2021 Jul 21;109(14):2256-2274.e9

Insert

BARKrgs peptide

Use

Tags

HA / FLAG

Expression

Mammalian

Mutation

Promoter

Available since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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VRQR variant

Plasmid

#138562

Purpose

Expresses human codon-optimized SpCas9-VRQR and blasticidin resistance: EFS promoter-VRQR-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

Insert

VRQR

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Mutation

D1135V, G1218R, R1335Q, T1337R

Promoter

EFS

Available since

June 9, 2020

Availability

Academic Institutions and Nonprofits only

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Cry2 mCherry RGS4(del 1-33) in pcDNA3.1

Plasmid

#64207

Purpose

mCherry fused between Cry2 and RGS4(del 1-33) to study cell migration

Depositor

Narasimhan Gautam

Article

O'Neill et al Mol Biol Cell. 2014 Aug 1;25(15):2305-14

Inserts

Cry2 (CRY2 Mustard Weed)
RGS4(del 1-33) (RGS4 Human)

Use

Tags

mcherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 19, 2015

Availability

Academic Institutions and Nonprofits only
GFP-Drosha218-1374

Plasmid

#62523

Purpose

N-terminal aa1-217 of Drosha is deleted

Depositor

Bharat Ramratnam

Article

Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

Insert

human Drosha with aa 1-217 deleted (DROSHA Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

deleted amino acids 1-217

Promoter

Available since

March 20, 2015

Availability

Academic Institutions and Nonprofits only