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Showing: 11921 - 11940 of 55029 results

  3. pJK429

    Plasmid
    #155376
    Purpose
    PphlF_A4NT in dCRv1, 1x cascade + [dCas9 + PA4-mVenus]
    Depositor
    Pamela Silver
    Article
    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81
    Inserts
    dCas9 (bacteria)
    PA4-mVenus
    PphlF_A4NT in dCas9 sgRNA oscillator v1
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    D10A H840A (catalytically inactive)
    Promoter
    Available since
    June 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    155376-plasmid-map-sequence-id-308997

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  4. pGBW-m4046970

    Plasmid
    #145753
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;HA
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145753-plasmid-map-sequence-id-311539

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  12. SARS-CoV spike all5KO mutant

    Plasmid
    #231921
    Purpose
    For expression of SARS-CoV spike protein with glycan deletion at N1080, N1116, N1140, N1155, N1176 by implementing Asn-to-Gln mutation
    Depositor
    Sriram Neelamegham
    Article
    Yang et al npj Viruses. 2025 Jan; 3(4). doi: 10.103
    Insert
    spike all5KO (S SARS-CoV)
    Use
    Tags
    C9 and CD5 signal peptide
    Expression
    Mammalian
    Mutation
    N1080Q, N1116Q, N1140Q, N1155Q, N1176Q
    Promoter
    CMV
    Available since
    Jan. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    231921-plasmid-map-sequence-id-459043

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  13. MERS-CoV spike all7KO mutant

    Plasmid
    #231924
    Purpose
    For expression of MERS-CoV spike protein with glycan deletion at N1176, N1213, N1225, N1241, N1256, N1277, N1288 by implementing Asn-to-Gln mutation
    Depositor
    Sriram Neelamegham
    Article
    Yang et al npj Viruses. 2025 Jan; 3(4). doi: 10.103
    Insert
    spike all7KO (S MERS-CoV)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    N1176Q, N1213Q, N1225Q, N1241Q, N1256Q, N1277Q, N…
    Promoter
    CMV
    Available since
    Jan. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    231924-plasmid-map-sequence-id-459044

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  16. pWR58

    Plasmid
    #202795
    Purpose
    integrating bioluminescene reporter for Cas9 activity in S. stipitis
    Depositor
    J. Brian Robertson
    Article
    Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:
    Inserts
    P-TDH3/coCBG(first portion)-YUM1
    P-TEF1/ShBle/T-ACT1
    YUM1-coCBG(last portion)/T-ADH2
    URA3 and flanking sequence
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Dec. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    202795-plasmid-map-sequence-id-455380

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Showing: 11921 - 11940 of 55029 results