-
Plasmid#88309PurposeDonor Vector containing ZNF32 transcription factor, part of the Human TFome CollectionDepositorInsertZNF32 (ZNF32 Human)
UseGateway donor vectorTagsExpressionMutationLast nucleotide of stop codon removed to allow fo…PromoterAvailable sinceJune 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-ZNF449
Plasmid#88453PurposeDonor Vector containing ZNF449 transcription factor, part of the Human TFome CollectionDepositorInsertZNF449 (ZNF449 Human)
UseGateway donor vectorTagsExpressionMutationLast nucleotide of stop codon removed to allow fo…PromoterAvailable sinceJune 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCOLADuet-1_nsp10 (SARS-CoV-2)
Plasmid#169158PurposeTo express SARS-CoV-2 nsp10 in E. coliDepositorInsertnsp10 (ORF1ab SARS-CoV-2)
UseTagsMethionineExpressionBacterialMutationCodon optimised for E. coliPromoterT7Available sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
MBP-hnRNPA2_deltaLC
Plasmid#98664PurposeExpresses MBP-tagged hnRNPA2 without the LC domainDepositorInserthnRNPA2 (HNRNPA2B1 Human)
UseTags6xHis-MBPExpressionBacterialMutationG190StopPromoterAvailable sinceJan. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAFNF-PSDd1.2-GFP
Plasmid#125581PurposeFlp-dependent expression of PSD d1.2-GFP (a deletion mutant of PSD-95 fused to GFP) in mammalian cells.DepositorInsertPSDd1.2-GFP (Dlg4 Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceJuly 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
UPRT-mCh-Nluc-P2A-neo-UPRT
Plasmid#135015PurposeExpresses mCherry protein and a fusion protein of Nluc-neo inserting into Cryptosporidium parvum UPRT locusDepositorInsertsmCh-Nluc-P2A-neo
UPRT 5'UTR
UPRT 3'UTR
UseCryptosporidium expressionTagsmCherry and Nluc-neoExpressionMutationPromoterCryptosporidium actin and enolaseAvailable sinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-ZNF716
Plasmid#101481PurposeDonor Vector containing ZNF716 transcription factor, part of the Human TFome CollectionDepositorInsertZNF716 (ZNF716 Human)
UseGateway shuttling vectorTagsExpressionMutationLast nucleotide of stop codon removed to allow fo…PromoterAvailable sinceJune 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV10-Flag-Ovtm
Plasmid#51888Purposeexpression vectore in mammalian cellsDepositorInsertmFbxl3 (Fbxl3 Mouse)
UseTagsFLAGExpressionMammalianMutationovtm (I364T)PromoterCMVAvailable sinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-ZNF655
Plasmid#88578PurposeDonor Vector containing ZNF655 transcription factor, part of the Human TFome CollectionDepositorInsertZNF655 (ZNF655 Human)
UseGateway donor vectorTagsExpressionMutationLast nucleotide of stop codon removed to allow fo…PromoterAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-ZNF680
Plasmid#88455PurposeDonor Vector containing ZNF680 transcription factor, part of the Human TFome CollectionDepositorInsertZNF680 (ZNF680 Human)
UseGateway donor vectorTagsExpressionMutationLast nucleotide of stop codon removed to allow fo…PromoterAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTT3-SP-6xHis-KIR2DL1(FL)-FLAG
Plasmid#157625PurposeMammalian cell surface expression of His- and FLAG-tagged full-length protein for binding and signaling assaysDepositorInsertKIR2DL1 (KIR2DL1 Human)
UseTags6xHis and FLAGExpressionMammalianMutationPromoterCMVAvailable sinceJan. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-FRT-TO-FH-Nsp8-Nsp7_GC3opt
Plasmid#157701Purposemammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp8-Nsp7 fusion protein under control of a tetracycline-inducible promoterDepositorInsertFH-Nsp8-Nsp7_GC3opt (ORF1ab SARS-CoV-2)
UseTagsFlag-His8ExpressionMammalianMutationcodon optimized; gene expression was optimized by…PromoterAvailable sinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR223-ZNF165
Plasmid#88444PurposeDonor Vector containing ZNF165 transcription factor, part of the Human TFome CollectionDepositorInsertZNF165 (ZNF165 Human)
UseGateway donor vectorTagsExpressionMutationLast nucleotide of stop codon removed to allow fo…PromoterAvailable sinceJune 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX SHIP1-SH2
Plasmid#46492DepositorInsertSHIP1 (INPP5D Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 1-118PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pNOC_dfnCas12a_crRNA NC
Plasmid#176256PurposepNOC episomal plasmid harboring the dead version of humanized fnCas12a gene sequence tagged with Nlux and spacer sequence is replaced by the type IIS restriction site for endonuclease BaeI that can beDepositorInserthumanized fnCas12a
UseCRISPR, Luciferase, and Synthetic Biology ; Expre…TagsluciferaseExpressionMutationD917A and E1006A mutations to inactivate the endo…PromoterAvailable sinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTT3-SP-6xHis-KIR2DL4(FL)-FLAG
Plasmid#157624PurposeMammalian cell surface expression of His- and FLAG-tagged full-length protein for binding and signaling assaysDepositorInsertKIR2DL4 (KIR2DL4 Human)
UseTags6xHis and FLAGExpressionMammalianMutationPromoterCMVAvailable sinceJan. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEMS1097
Plasmid#29028PurposeExpression of the reporter gene under the control of the MiniPromoter in this plasmid has not been testedDepositorInsertPle201 (SLC6A5 Human)
UsePleiades promoter project [sic, pleaides plieades]TagsEGFP-Cre-NLSExpressionMutationPromoterAvailable sinceJan. 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
MBP-hnRNPA2_FL_D290V
Plasmid#98663PurposeExpresses MBP-tagged full length hnRNPA2 with D290VDepositorInserthnRNPA2 (HNRNPA2B1 Human)
UseTags6xHis-MBPExpressionBacterialMutationD290VPromoterAvailable sinceJan. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-ZNF467
Plasmid#88643PurposeDonor Vector containing ZNF467 transcription factor, part of the Human TFome CollectionDepositorInsertZNF467 (ZNF467 Human)
UseGateway donor vectorTagsExpressionMutationLast nucleotide of stop codon removed to allow fo…PromoterAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
NC4 pCDNA3.1 VENUS WTX (1-804)
Plasmid#36959DepositorInsertWTX (AMER1 Human)
UseTagsVenusExpressionMammalianMutationPromoterCMVAvailable sinceJuly 24, 2012AvailabilityAcademic Institutions and Nonprofits only