Showing: 11901 - 11920 of 17306 results
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Plasmid#186362PurposeEntry clone with ORF encoding Ciona robusta Aurora kinase A flanked by Gateway recombination sequences. No Stop sequence: to be recombined into a destination vector with a C-terminal protein tag.DepositorInsertCiona robusta Aurora kinase A
UseExpression of a fluorescent centrosome markerTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pVRC8400-SARS-CoV-2-S2P-BA.4/5-AVI
Plasmid#192112PurposeExpresses SARS-CoV-2 S2P protein from the BA.4 and BA.5 variants with single chain Fc, HRV3C protease cleavage site, and AVI tagDepositorInsertSARS-CoV-2-S2P-BA.4/5 (S )
UseTagsAVI, HRV3C, and scFcExpressionMammalianMutationT19I, L24S, P25-, P26-, A27-, H69-, V70-, G142D, …PromoterAvailabilityAcademic Institutions and Nonprofits only -
pET28 sf:em-blue-shift.2
Plasmid#191919PurposeEncodes a GFP design, sf:em-blue-shift.2DepositorInsertsf:em-blue-shift.2
UseTagsHis TagExpressionBacterialMutationT65S S72A T108I Y145F V150IPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pET28 sf:em-blue-shift.1
Plasmid#191928PurposeEncodes a GFP design, sf:em-blue-shift.1DepositorInsertsf:em-blue-shift.1
UseTagsHis TagExpressionBacterialMutationT65S Q69P S72A T108V Y145M T167V V224IPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
MSCV-HA-Nr4a1
Plasmid#160941PurposeGeneration of retrovirus for the overexpression of Nr4a1DepositorInsertNr4a1 (Nr4a1 Mouse)
UseRetroviralTagsHA tagExpressionMammalianMutationPromoterMSCV-LTRAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-FluoSTEP-JNKAR
Plasmid#181857PurposeGenetically encoded FRET-based sensor for monitoring JNK kinase activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.DepositorInsertFluoSTEP-JNKAR
UseTagsmRuby2 and sfGFP(1-10)ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-FluoSTEP-EKAR
Plasmid#181858PurposeGenetically encoded FRET-based sensor for monitoring ERK activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.DepositorInsertFluoSTEP-EKAR
UseTagsmRuby2 and sfGFP(1-10)ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FluoSTEP-AKAR (C/Y)
Plasmid#181862PurposeFluoSTEP-AKAR using split CFP and cpVenus(E172) as the FRET donor and acceptor. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.DepositorInsertFluoSTEP-AKAR (C-Y)
UseTagsCFP(1-10) and cpVenus(E127)ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pET-NlaCascade-NLS-6xHis
Plasmid#180213PurposeExpressing Neisseria lactamica Type I-C Cascade subunits (Cas5-Cas8-Cas11-Cas7) with NLS and His tag on the C terminus of Cas7 for protein purification in E.ColiDepositorInsertNlaCas5-Cas8-Cas7
UseTags6XHis and NLSExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pCDNA_MET_3Flag
Plasmid#182496PurposeContains cDNA of wt METDepositorInsertMET (MET Human)
UseTagsC-terminal 3xFlag tagExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
TDP-43 _CTD_5M→L_IDR
Plasmid#194265PurposeExpresses 6xHis-tagged C-terminal domain of TDP-43 with 5 Methionines to leucineDepositorInsertTDP-43 (TARDBP Human)
UseTags6XHistagExpressionBacterialMutationM307L, M311L, M359L, M405L, M414L mutations in TD…PromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pHis-WzzE
Plasmid#196661PurposeExpression of N-terminally His6-tagged WzzE from Pectobacterium atrosepticum in E.coliDepositorInsertWzzE
UseTagsTEV-cleavable His6ExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pcmv3-STING-C1471S-FLAG
Plasmid#198188PurposeMammalian expression of FLAG-tagged mouse STING1 C1471SDepositorInsertTMEM173 (Sting1 Mouse)
UseTagsFLAGExpressionMammalianMutationThe cysteine in STING at position 147 mutates to …PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
iRFP713-N2-Frankenbody-Flag
Plasmid#175291PurposeVisualize endogeneous loci coupled with ZF probes harboring 10xFLAG tagDepositorInsertanti-FLAG frankenbody fused with iRFP713
UseTagsiRFP713ExpressionMammalianMutationPromoterAvailabilityIndustry, Academic Institutions, and Nonprofits -
mCherry-N2-Frankenbody-HA
Plasmid#175292PurposeVisualize endogeneous loci coupled with ZF probes harboring 10xHA tagDepositorInsertanti-HA frankenbody fused with mCherry
UseTagsmCherryExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
221-pCAGGS-HA-AID-CTCFcDNA-AID-eGFP-2A-blasticidin
Plasmid#195101PurposeVector to introduce human CTCF cDNA tagged with eGFP and two AIDs in the cells. Blasticidin selection (2A-fusion). Auxin-inducible degron system.DepositorInsertCTCF
UseTagsAID, HA, and eGFPExpressionMammalianMutationPromoterpCAGGSAvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-HA_aa 1-420 POLI
Plasmid#201582PurposeExpresses the catalytic domain (aa 1-420) of human DNA polymerase iota with a HA tag in mammalian cellsDepositorInsertDNA polymerase iota (POLI Human)
UseTagsHAExpressionMammalianMutationExpresses amino acids 1-420 of DNA polymerase iotaPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-HA_aa 1-449 POLH
Plasmid#201583PurposeExpresses the catalytic domain (aa 1-449) of human DNA polymerase eta with a HA tag in mammalian cellsDepositorInsertPOLH (POLH Human)
UseTagsHAExpressionMammalianMutationExpresses amino acids 1-449 of DNA polymerase etaPromoterAvailabilityAcademic Institutions and Nonprofits only -
pWPI-GFP-3xFLAG
Plasmid#201639PurposeOver-expression of 3xFLAG-tagged GFPDepositorInsertGFP
UseLentiviralTags3xFLAGExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pWPI-SLC7A11-HA
Plasmid#201643PurposeOver-expression of HA-tagged human SLC7A11DepositorInsertSLC7A11 (SLC7A11 Human)
UseLentiviralTagsHAExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 11901 - 11920 of 17306 results