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Plasmid#191676Purposetransfer plasmid for lentiviral vector production expressing Hs ATP10B D433N mutant and His/flag tagged Hs TMEM30ADepositorUseLentiviralTags6x HIS and flagExpressionMutationD433NPromoterAvailable sinceNov. 17, 2022AvailabilityAcademic Institutions and Nonprofits only
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pCFJ909
Plasmid#44480DepositorInsertMinimal Mos1, unc-119, MCS
UseMultiple cloning site vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Mycn-IRES-RFP
Plasmid#35393DepositorInsertsUseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCMV-AAAAA-nCas9(D10A)-UGI
Plasmid#187409PurposeDddA AAAAA variantDepositorInsertDddA AAAAA
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-TK-Firefly-cjBCCIP
Plasmid#122278PurposeLentiviral vector for stable expression of Firefly luciferase.DepositorInsertsFirefly luciferase
cjBCCIP 3'UTR
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceApril 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVITRO-HPV40 L1L2
Plasmid#52593PurposeExpresses HPV40 L1 and L2 in mammalian cellsDepositorInsertsHPV40 L1
HPV40 L2
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pVITRO-HPV44 L1L2
Plasmid#52597PurposeExpresses HPV44 L1 and L2 in mammalian cellsDepositorInsertsHPV44 L1
HPV44 L2
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMVS-V1
Plasmid#183094PurposeBasic conjugative shuttle vector for Escherichia coli and Methanothermobacter thermautotrophicusDepositorInsertspME2001
thermostable neomycin phosphotransferase gene
UseBacterial propagation and conjugativeTagsExpressionMutationPromoterAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCas1_Cas2/3
Plasmid#89240PurposePlasmid contains the genes Cas1 and Cas2/3, cloned from Pseudomonas aeruginosa as a polycistronic operon. Cas1 includes an N-terminal Strep II tag and TEV protease cleavage site on Cas1DepositorInsertCas1 and Cas2/3
UseTagsStrep II tag on Cas1ExpressionMutationPromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTK313
Plasmid#59570PurposeTC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP XbaI attB1 Kozak EGFP-PEST ochre attB2 AscI (truncated E1) 3'UTR Poly A I-SceIDepositorInsertSGP XbaI attB1 Kozak EGFP-PEST ochre attB2 AscI
UseTagsExpressionMutationPromoterAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pVITRO-HPV38 L1L2
Plasmid#52594PurposeExpresses HPV38 L1 and L2 in mammalian cellsDepositorInsertsHPV38 L1
HPV38 L2
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
141_pT3TS_iCre
Plasmid#171793PurposePlasmid for optimised iCre RNA synthesis using T3 promoter.DepositorInsertoptimised Cre for RNA expression
UseTagsExpressionBacterialMutationPromoterT3Available sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPRv2puro-Tyr
Plasmid#170293PurposeA knock-out vector for the mouse tyrosinase.DepositorInsertA gRNA targeting the mouse tyrosinase gene.
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-LbCpf1-U6_FlipArray
Plasmid#109349PurposeLentiviral vector to express Cpf1 from EFS promoter, as well as a Cpf1 FlipArray from U6 promoter.DepositorInsertU6-FlipArray
UseCRISPR, Cre/Lox, Lentiviral, and Synthetic Biolog…TagsExpressionMammalianMutationPromoterU6Available sinceJuly 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSLHDR03_mCherry_tCD19_unique guide
Plasmid#124062PurposeConstruction of 1st donor for scarless genome editing with unique guideDepositorInsertsmCherry
tCD19
UseMouse Targeting; Human targetingTagsExpressionMutationPromoterHuman UbC modifiedAvailable sinceApril 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAH237
Plasmid#121146PurposeExpression of both nmt41p-cas9 and rrk1p-gRNA (LEU2 marker) for CRISPR genome editing in fission yeastDepositorInsertsgRNA cassette
humanized Streptococcus pyogenes Cas9
UseCRISPRTagsFlagExpressionYeastMutationPromoterS.pombe rrk1 and nmt41Available sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFC331
Plasmid#87843PurposeAMA1 plasmid with Aspergillus optimized Cas9 and argB selection markerDepositorInsertsCas9
argB
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialMutationPromoterAspergillus nidulans tef1 promoter and native pro…Available sinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pVITRO-HPV34 L1L2
Plasmid#52591PurposeExpresses HPV34 L1 and L2 in mammalian cellsDepositorInsertsHPV34 L1
HPV34 L2
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
HB401: pMVP (L1-L4) CMV promoter
Plasmid#121686PurposepMVP L1-L4 entry plasmid, contains CMV promoter for 3-component MultiSite Gateway Pro assemblyDepositorInsertCMV promoter
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTC223
Plasmid#70019PurposeCas9/sgRNA targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon based on Bean Yellow Dwarf Virus; sgRNA= 7DepositorInsertNuclease (Cas9/sgRNA) + Donor + GVR
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceDec. 1, 2015AvailabilityAcademic Institutions and Nonprofits only