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Plasmid#104635PurposeLevel 2 constructDepositorInsertHIS-Ubq_UBA1_HA-UBC8_GST-AvrPtoB_EL4
UseTagsHIS-Ubq, HA-E2, GST-E3ExpressionBacterialMutationPromoterT7Available sinceFeb. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
Flag-HA-USP39
Plasmid#22581DepositorInsertUSP39 (USP39 Human)
UseRetroviralTagsFLAG and HAExpressionMammalianMutationNonePromoterAvailable sinceNov. 16, 2009AvailabilityAcademic Institutions and Nonprofits only -
pDDGFP_LEU2D
Plasmid#58352PurposeUsed for expressing recombinant proteins as a C terminal GFP-His tagged constuct in S.cerevisiaeDepositorTypeEmpty backboneUseTagsGFP-HisExpressionYeastMutationPromoterGAL1Available sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a-nanoHER2-SpyTag003
Plasmid#216312PurposeExpresses nanoHER2-SpyTag003 in bacterial cells. nanoHER2 is an anti-HER2 nanobody.DepositorInsertnanoHER2-SpyTag003
UseTagsHis6 tag and SpyTag003ExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETM43 p62 FL (1-440)
Plasmid#210425PurposeBacterial expression of MBP-p62 FL (1-440) with 3C cleavage siteDepositorInsertp62 FL (NUP62 Human)
UseTags6xHis and MBPExpressionBacterialMutationPromoterAvailable sinceFeb. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBB535
Plasmid#27392DepositorUseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 28, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMF230
Plasmid#62546PurposeBroad host range plasmid for constitutive expression of the eGFP. Developed for imaging Pseudomonas aeruginosa in biofilms.DepositorInsertGFP mut2
UseBroad host range plasmidTagsExpressionMutationPromotertrcAvailable sinceApril 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSEVA1213S
Plasmid#122095Purposeconstitutive expression of I-SceI for genome engineering in PseudomonasDepositorInsertI-SceI restriction endonuclease
UseTagsExpressionMutationPromoterAvailable sinceMay 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAG413 GAL ORF1 Shuffle1 NLS
Plasmid#145794PurposemPing Yeast Transposition AssayDepositorInsertORF1 Shuffle1 NLS
UseTagsExpressionYeastMutationEnhanced Nucleur Localization SignalPromoterAvailable sinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pMyNT-kan
Plasmid#84693PurposeProtein expression in mycobacteriaDepositorTypeEmpty backboneUseTagsHis6ExpressionBacterialMutationPromoterAcetamidaseAvailable sinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
Flag-HA-USP8
Plasmid#22608DepositorInsertUSP8 (USP8 Human)
UseRetroviralTagsFLAG and HAExpressionMammalianMutationNonePromoterAvailable sinceDec. 16, 2009AvailabilityAcademic Institutions and Nonprofits only -
Cherry-LacRep
Plasmid#18985DepositorInsertLacI
UseTagsAviTag, SV40 NLS, and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceAug. 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
pSN829
Plasmid#190691PurposeMammalian expression of kinesin scFv(H2) fused with the Fc domain of mouse IgG2DepositorInsertkinesin scFv(H2)::Fc(mouse IgG2)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMyC-kan
Plasmid#84692PurposeProtein expression in mycobacteriaDepositorTypeEmpty backboneUseTagsHis6ExpressionBacterialMutationPromoterAcetamidaseAvailable sinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
6975 pPROEX-HTb-SIS1
Plasmid#1233DepositorInsertsis1 (SIS1 Budding Yeast)
UseTagshis tag (tev cleavable)ExpressionBacterialMutationPromoterAvailable sinceJuly 18, 2005AvailabilityAcademic Institutions and Nonprofits only -
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pBHt2
Plasmid#104175PurposeEmpty backbone for Agrobacterium tumefaciens-mediated transformation of Fusarium oxysporumDepositorTypeEmpty backboneUseFungal expressionTagsExpressionMutationPromoternoneAvailable sinceJan. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
VN-Tau (P301L)
Plasmid#87634Purposeexpresses hTau fused to the N-terminal part of VenusDepositorInsertMAPT (MAPT Human)
UseTagsN-terminal part of VenusExpressionMammalianMutationMutation P301LPromoterCMVAvailable sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMBI
Plasmid#17816DepositorInsertmevalonate kinase, phosphomevalonate kinase, mevalonate pyrophosphate decarboxylatse, IPP isomerase
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 28, 2008AvailabilityAcademic Institutions and Nonprofits only