Addgene

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pcDNA3 Lunatic Fringe

Plasmid

#24962

Depositor

Edward De Robertis

Article

Pera et al Int J Dev Biol. 2005 . 49(7):781-96.

Insert

Lunatic Fringe (lfng Frog)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 18, 2010

Availability

Academic Institutions and Nonprofits only
pSicoR

Plasmid

#11579

Depositor

Tyler Jacks

Article

Ventura et al Proc Natl Acad Sci U S A. 2004 Jul 13. 1

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Cre/Lox, Lentiviral, and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 3, 2006

Availability

Academic Institutions and Nonprofits only

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pROP-PP-FBFP

Plasmid

#122137

Purpose

FMN-based fluorescent protein Reporter plasmid for B-Rex contain perfect operator sequence (anaerobic purpose)

Depositor

Srivatsan Raman

Article

Liu et al ACS Synth Biol. 2019 Feb 15;8(2):264-273

Insert

FBFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

J23101

Available since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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pAC1816_pCR8-gSMN2-DN4

Plasmid

#118649

Purpose

Transient transfection; Expressed SMN2-DN4 CasRx gRNA; Gateway Donor

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

gSMN2-DN4

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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mRFP1-N1

Plasmid

#54635

Purpose

Localization: N1 Cloning Vector, Excitation: 584, Emission: 607

Depositor

Michael Davidson, Robert Campbell, Roger Tsien

Article

Campbell et al Proc Natl Acad Sci U S A. 2002 Jun 11;99

Type

Empty backbone

Use

Tags

mRFP1

Expression

Mammalian

Mutation

Promoter

Available since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

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pQCXIN-TetR-mCherry

Plasmid

#59417

Purpose

retroviral construct expressing the Tet repressor (TetR) tagged with mCherry

Depositor

Tom Misteli

Article

Roukos et al Science. 2013 Aug 9;341(6146):660-4. doi

Insert

Tet repressor protein (TetR)

Use

Retroviral

Tags

mCherry

Expression

Mutation

Promoter

Available since

Nov. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pFastBac1 hBRM

Plasmid

#1956

Depositor

Robert Kingston

Article

Phelan et al Mol Cell 1999 Feb;3(2):247-53.

Insert

BRM (SMARCA2 Human)

Use

Tags

flag

Expression

Insect

Mutation

Promoter

Available since

May 24, 2006

Availability

Academic Institutions and Nonprofits only
pZ8-T_dCas9

Plasmid

#74062

Purpose

pZ8-1 plasmid carrying dcas9, driven by the IPTG-inducible Ptac promoter, KanR

Depositor

Timothy Lu

Article

Cleto et al ACS Synth Biol. 2016 Feb 16.

Insert

dcas9

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

ptac

Available since

April 11, 2016

Availability

Academic Institutions and Nonprofits only

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Flag-hPIASy

Plasmid

#15208

Depositor

Ke Shuai

Article

Liu et al Proc Natl Acad Sci U S A. 2001 Mar 13. 9

Insert

PIASy (PIAS4 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 8, 2008

Availability

Academic Institutions and Nonprofits only

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pCMV5B-Smad4

Plasmid

#11743

Depositor

Jeff Wrana

Article

Macias-Silva et al Cell. 1996 Dec 27. 87(7):1215-24.

Insert

SMAD4 (Smad4 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 7, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3-Flag-Smad4

Plasmid

#80888

Purpose

mammalian expression of Smad4

Depositor

Aristidis Moustakas

Article

Moren et al Oncogene. 2000 Sep 7;19(38):4396-404.

Insert

Smad4 (SMAD4 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pMJH46

Plasmid

#67272

Purpose

Conjugally transferable red recombinogenic plasmid

Depositor

Mark Liles

Article

Hossain et al Biotechnol Rep (Amst). 2015 Aug 28;8:24-

Insert

traJ, cat, oriT

Use

Red recombinase

Tags

Expression

Mutation

Promoter

cat gene promoter from PGNS-BAC

Available since

Sept. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pJet1.2 ermB cassette

Plasmid

#117121

Purpose

Erythromycin resistance cassette

Depositor

Stephan Gruber

Article

Diebold-Durand et al Methods Mol Biol. 2019;2004:49-61. doi:

Insert

Erythromycin resistance cassette for Bacillus subtilis selection

Use

Golden gate donor vector for gene targeting in ba…

Tags

Expression

Mutation

Promoter

Available since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pQLinkHD_ECFP

Plasmid

#118857

Purpose

fluorescent protein expression with an N-terminal His-tag

Depositor

Marc Birtwistle

Article

Holzapfel et al ACS Comb Sci. 2018 Nov 12;20(11):653-659

Insert

ECFP

Use

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pJet1.2 cat cassette

Plasmid

#117119

Purpose

Chloramphenicol resistance cassette

Depositor

Stephan Gruber

Article

Diebold-Durand et al Methods Mol Biol. 2019;2004:49-61. doi:

Insert

Chloramphenicol resistance cassette for Bacillus subtilis selection

Use

Golden gate donor vector for gene targeting in ba…

Tags

Expression

Mutation

Promoter

Available since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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p109.1

Plasmid

#44518

Depositor

Michael Grunstein

Article

Hecht et al Cell. 1995 Feb 24;80(4):583-92.

Insert

Histone 4

Use

Tags

GST

Expression

Bacterial

Mutation

contains full length (aa 1-102)

Promoter

Available since

May 21, 2013

Availability

Academic Institutions and Nonprofits only
pME-GAL4-ERT2-VP16

Plasmid

#82588

Purpose

Middle entry vector containing GAL4 fused to ERT2 and the VP16 transcriptional activation domain for tamoxifen-inducible UAS-promoted transgene expression

Depositor

Kryn Stankunas

Article

Akerberg et al PLoS One. 2014 Mar 18;9(3):e92217. doi:

Insert

GAL4-ERT2-VP16

Use

Tags

Expression

Mutation

Promoter

None

Available since

Dec. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pSO2002

Plasmid

#25842

Depositor

Aziz Sancar

Article

Ozgur et al Biochemistry. 2003 Mar 18. 42(10):2926-3

Insert

human Cryptochrome 2 (CRY2 Human)

Use

Tags

flag, his, and myc

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 9, 2010

Availability

Academic Institutions and Nonprofits only

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pPS2351

Plasmid

#1352

Depositor

Pamela Silver

Article

Hitchcock et al Mol Biol Cell 2001 Oct;12(10):3226-41.

Insert

MGA2 (MGA2 Budding Yeast)

Use

Tags

GFP

Expression

Yeast

Mutation

Promoter

Available since

Aug. 3, 2005

Availability

Academic Institutions and Nonprofits only
pH2B_mCherry_IRES_puro2

Plasmid

#21045

Depositor

Daniel Gerlich

Article

Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

Insert

pH2B (H2BC16P Human)

Use

Tags

mCherry

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

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