-
Plasmid#102975PurposeSuppression of SOD1DepositorInsertshSOD1 (SOD1 Human)
UseLentiviral and RNAiTagsExpressionMutationPromoterAvailable sinceNov. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.0_nucNanobody IRES EGFP
Plasmid#136619PurposePlasmid encoding nuclear localization of the Enhancer nanobody for GFP as a SNAP-Tag-fusion and EGFPDepositorInsertSNAP-Tag-EnhancerNb-NLS (IRES EGFP)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 GDI2
Plasmid#67093PurposeStandard for cell-free expression benchmarking.DepositorInsertGDI2 (GDI2 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2TK-14-3-3 beta GST
Plasmid#13276DepositorInsert14-3-3 beta (YWHAB Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceOct. 7, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 CALR
Plasmid#67086PurposeStandard for cell-free expression benchmarking.DepositorInsertCALR (CALR Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_HSPA9-Halo
Plasmid#175332PurposeProduction of HaloTag fusion proteinDepositorInsertHSPA9 (HSPA9 Human)
UseCell free expressionTagsHaloTag-6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDS24
Plasmid#58952PurposeEncodes synthetic mitochondrial gene ARG8m in place of COX3 coding sequence. Flanked by mtDNA sequences allowing integration into rho+ mtDNA after transformation.DepositorInsertMitochondrially coded Arg8 protein
UseTagsExpressionMutationCoding sequence synthetic in S. cerevisiae mitoch…Promotern/aAvailable sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pPS22 (pET23a-Cas10/Csm/csm2H6N)
Plasmid#70038PurposeBacterial expression of the S. epidermidis Cas10·Csm complexDepositorInsertCas10/Csm complex
UseTags6xHIS on N terminus of Csm2ExpressionBacterialMutationPromoterT7Available sinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pX097-U6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry
Plasmid#41863PurposeThis plasmid is used to reconstitute the complete Type II CRISPR system from S. pyogenes and contains the host factor RNaseIII and tracrRNA.DepositorArticleInsertU6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_EGFP-LAMA-R59 (for ALFA-tag)
Plasmid#136627PurposePlasmid encoding the bacterial expression of LAMA-R59 for ALFA-tag as an EGFP-fusionDepositorInsertEGFP-LAMA-R59
UseTagsHisx10 and StrepExpressionBacterialMutationPromoterT7Available sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCoofy44
Plasmid#55185PurposeInsect expression vector for parallel SLIC cloning containing N-terminal 10x His and SumoStar tags. C-terminal tags can be added during SLIC.DepositorTypeEmpty backboneUseTags10x His, 10x His-Stop-OneStrep-Stop-S-tag-Stop-HP…ExpressionBacterialMutationPromoterpPH (Polyhedrin promoter)Available sinceJuly 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pActin-miRFP670nano3
Plasmid#184665PurposeHuman beta-actin labelled with near-infrared fluorescent protein miRFP670nano3DepositorInsertmiRFP670nano3-human beta-actin
UseTagsmiRFP670nano3ExpressionMammalianMutationPromoterCMVAvailable sinceJune 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pASKt15C+SrtA7
Plasmid#64986PurposeExpresses the P94R/E105K/E108A/D160N/D165A/K190E/K196T mutant of Staphylococcus aureus sortase A in E. coliDepositorInsertsortase A
UseTagsHis6 tag and S tagExpressionBacterialMutationdeleted amino acids 1-59, P94R, E105K, E108A, D16…Promotertetracycline promoter/operatorAvailable sinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNIR-FbGFPY58/C-JIP
Plasmid#184684PurposeNIR-Fb-based JNK inhibitorDepositorInsertNIR-FbGFPY58/C-JIP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.0_nucLAMA-F98 IRES EGFP
Plasmid#136618PurposePlasmid encoding nuclear localization of LAMA-F98 for GFP as a SNAP-Tag-fusion and EGFPDepositorInsertSNAP-Tag-LAMA-F98-NLS (IRES EGFP)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4136464
Plasmid#149534PurposeBacterial expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTags6xHIS;Linker_GGSG;GST;Linker_GGSG;Cleavable TEVExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pNIR-FbGFPY58/C-PKI
Plasmid#184685PurposeNIR-Fb-based PKA inhibitorDepositorInsertNIR-FbGFPY58/C-PKI
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSHS306 - Bacterial expression plasmid for SpCas9, HNH FRET variant
Plasmid#87350PurposeS867C/S355C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, HNH-1 FRET constructDepositorInsertCas9
UseTagsHis-MBPExpressionBacterialMutationC80S/S355C/C574S/S867CPromoterT7Available sinceMarch 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4136461
Plasmid#149538PurposeBacterial expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTags6xHIS;Linker_GGSG;MBP;Linker_GGSG;Cleavable TEVExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046758
Plasmid#145670PurposeBacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;HAExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits