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pCMV-Bx

Plasmid

#51552

Purpose

Expresses Bxb1 integrase in mammalian cells.

Depositor

Michele Calos

Article

Zhu et al Nucleic Acids Res. 2013 Dec 4.

Insert

Bxb1 integrase

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 3, 2014

Availability

Academic Institutions and Nonprofits only

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EGFP-Rab5A S34N

Plasmid

#28045

Depositor

Qing Zhong

Article

Sun et al Proc Natl Acad Sci U S A. 2010 Nov 9. 10

Insert

Rab5A (RAB5A Human)

Use

Tags

EGFP on N-term

Expression

Mammalian

Mutation

S34N

Promoter

Available since

Feb. 17, 2011

Availability

Academic Institutions and Nonprofits only

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pBSKrnaSeph

Plasmid

#67506

Purpose

Express the tRNALys scaffold with the sephadex aptamer tag

Depositor

Luc Ponchon

Article

Ponchon et al Nat Protoc. 2009;4(6):947-59. doi: 10.10

Insert

modified tRNALys with sephadex aptamer

Use

Tags

Expression

Bacterial

Mutation

Promoter

lpp

Available since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
pBABE-ddIPpoI

Plasmid

#49052

Purpose

Expresses the restriction enzyme under the control of Shield1

Depositor

Michael Kastan

Article

Goldstein et al Proc Natl Acad Sci U S A. 2013 Sep 30.

Insert

DD-HA-ER-IPpoI

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 25, 2013

Availability

Academic Institutions and Nonprofits only

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pYes2-TaPCS1

Plasmid

#49767

Purpose

Expresses wheat phytochelatin synthase (TaPCS1) in Saccharomyces Cerevisiae

Depositor

Julian Schroeder

Article

Clemens et al EMBO J. 1999 Jun 15;18(12):3325-33.

Insert

TaPCS1 (PCS1 Triticum aestivum)

Use

Tags

Expression

Yeast

Mutation

Promoter

GAL1

Available since

Jan. 16, 2014

Availability

Academic Institutions and Nonprofits only
pEGFP-C1-UBF

Plasmid

#26672

Depositor

Sui Huang

Article

Chen et al J Cell Biol. 2001 Apr 2. 153(1):169-76.

Insert

UBF1 (UBTF Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 10, 2010

Availability

Academic Institutions and Nonprofits only
pMJH65

Plasmid

#67273

Purpose

Conjugally transferable red recombinogenic plasmid

Depositor

Mark Liles

Article

Hossain et al Biotechnol Rep (Amst). 2015 Aug 28;8:24-

Insert

tetA

Use

Red recombinase, temperature sensitive

Tags

Expression

Bacterial

Mutation

Promoter

tetA promoter of plasmid pACYC184 origin

Available since

Sept. 3, 2015

Availability

Academic Institutions and Nonprofits only

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HA-ISceID44A

Plasmid

#59424

Purpose

Expresses a catalytically inactive form of the endonuclease ISceI

Depositor

Tom Misteli

Article

Roukos et al Science. 2013 Aug 9;341(6146):660-4. doi

Insert

endonuclease ISceI mutant (I-SceI )

Use

Tags

HA tag

Expression

Mammalian

Mutation

changed aspartate 44 to alanine (D44A)

Promoter

Available since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pRGD-SmR

Plasmid

#74109

Purpose

Plasmid containing streptomycin resistance gene cassette flanked by multiple restriction sites

Depositor

Hans-Martin Fischer

Article

Ledermann et al Appl Environ Microbiol. 2016 Feb 26. pii

Insert

aadA

Use

Bacterial vector based on pmb1 replicon

Tags

Expression

Mutation

Promoter

Available since

April 15, 2016

Availability

Academic Institutions and Nonprofits only

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pTAKN2_J23119-sgRNA

Plasmid

#108552

Purpose

Bacterial sgRNA expression vector

Depositor

Akihiko Kondo

Article

Banno et al Nat Microbiol. 2018 Feb 5. pii: 10.1038/

Insert

2x Streptococcus pyogenes sgRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

J23119

Available since

May 3, 2018

Availability

Academic Institutions and Nonprofits only

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pJKR-H-acuR

Plasmid

#62567

Purpose

Acrylate biosensor. High copy plasmid that expresses GFP proportional to acrylate concentration.

Depositor

George Church

Article

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

GFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pHCMV-MokolaG

Plasmid

#15811

Depositor

Miguel Sena-Esteves

Article

Sena-Esteves et al J Virol Methods. 2004 Dec 15. 122(2):131

Insert

Mokola virus glycoprotein (G) gene

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 21, 2008

Availability

Academic Institutions and Nonprofits only

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SUMO-KTag

Plasmid

#51723

Purpose

Plasmid for recombinant expression in bacteria of SUMO domain fused to KTag, permitting covalent linkage to SpyTag

Depositor

Mark Howarth

Article

Fierer et al Proc Natl Acad Sci U S A. 2014 Mar 17.

Insert

SUMO-KTag

Use

Tags

His6 Tag

Expression

Bacterial

Mutation

none

Promoter

T7

Available since

May 1, 2014

Availability

Academic Institutions and Nonprofits only
pCRISPRainbow-DONOR1

Plasmid

#75398

Purpose

CRISPRainbow-DONOR1

Depositor

Thoru Pederson

Article

Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

Insert

Cm(R)-CcdB

Use

CRISPR

Tags

no

Expression

Mutation

Promoter

Bacteria

Available since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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GG-dest

Plasmid

#69538

Purpose

Destination plasmid for gRNA concatenation Golden Gate cloning

Depositor

Timo Otonkoski

Article

Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5

Type

Empty backbone

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pJet1.2 specR cassette

Plasmid

#117120

Purpose

Spectinomycin resistance cassette

Depositor

Stephan Gruber

Article

Diebold-Durand et al Methods Mol Biol. 2019;2004:49-61. doi:

Insert

Spectinomycin resistance cassette for Bacillus subtilis selection

Use

Golden gate donor vector for gene targeting in ba…

Tags

Expression

Mutation

Promoter

Available since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pMyrPalm_mEGFP

Plasmid

#21037

Depositor

Daniel Gerlich

Article

Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

Insert

pMyrPalm

Use

Tags

mEPFP

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

May 12, 2009

Availability

Academic Institutions and Nonprofits only
pMXs-Sox2-IP

Plasmid

#15919

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

SRY-box containing gene 2 (Sox2 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

None

Promoter

Available since

Feb. 8, 2008

Availability

Academic Institutions and Nonprofits only

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pBSK

Plasmid

#67504

Purpose

Express the tRNALys scaffold

Depositor

Luc Ponchon

Article

Ponchon et al Nat Protoc. 2009;4(6):947-59. doi: 10.10

Insert

modified tRNALys human

Use

Tags

Expression

Bacterial

Mutation

Promoter

lpp

Available since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
pRK-Myc-Smurf2

Plasmid

#13678

Depositor

Ying Zhang

Article

Zhang et al Proc Natl Acad Sci U S A. 2001 Jan 30. 9

Insert

Smurf2 (SMURF2 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 8, 2007

Availability

Academic Institutions and Nonprofits only