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Plasmid#46384DepositorInsertEWSR1 (EWSR1 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceJuly 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CMV-mCherry-hPMCA2w/b
Plasmid#111570PurposeExpresses a plasma membrane calcium pump hPMCA2w/bDepositorInsertPlasma membrane calcium pump 2, variant w/b (ATP2B2 Human)
UseTagsmCherryExpressionBacterialMutationPromoterAvailable sinceSept. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
ML14
Bacterial Strain#61911PurposeC43(DE3)-based E. coli amino acid auxotrophic host strains used for selective isotope labeling. tyrA gene deleted.DepositorBacterial ResistanceNoneAvailable sinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
MF84_PB-14xUAS-6x42bs-6x(BCRbs_BCRbs)-miniCMV-NLS-FKBP12F36V-BCRZFR39A-VP16-NLS-DHFR-IRES-mCherry-PEST-BGHpA
Plasmid#176628PurposeTF A self-activation cassette in MultiFate-2.3DepositorInsert14xUAS-6x42bs-6x(BCRbs_BCRbs)-miniCMV-NLS-FKBP12F36V-BCRZFR39A-VP16-NLS-DHFR-IRES-mCherry-PEST-BGHpA
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST_6xUAS:mNeonGreen-Rab5a
Plasmid#192355PurposezfRabaa tagged with mNeonGreen, expressed under the UAS promoterDepositorInsertRab5a (rab5aa Zebrafish)
UseTagsmNeonGreenExpressionMammalianMutationPromoterAvailable sinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET23b-2E2-HA-mEGFP-6xHis
Plasmid#129594PurposeThe encoded protein is the anti-HA frankenbody variant fused with the mEGFP and His-tag. This plasmid is for bacteria protein expression under T7 promoter and protein purification with HisTrap.DepositorInsertAnti-HA frankenbody variant-mEGFP (2E2-HA scFv-mEGFP)
UseTags6xHis-tagExpressionBacterialMutationPromoterT7Available sinceOct. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTRY-GFP
Plasmid#15301DepositorInserthrGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
hAAVS1 1L TALEN
Plasmid#35431PurposeTALEN designed to target the human AAVS1 locusDepositorInserthAAVS1 1L TALEN
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
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NLSx3-CRY2PHR-p65
Plasmid#64124PurposePhotoactivatable transcription system. Expression of activator probe, which includes the photolyase homology region of CRY2 (CRY2PHR) and the transcriptional activator domain.DepositorInsertCRY2PHR-p65
UseTagsHis, NLSx3, and V5ExpressionMammalianMutationPromoterCMVAvailable sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGL3-TGFb1
Plasmid#101762PurposeTGFb1 promoter reporterDepositorInserttransforming growth factor beta 1 promoter (TGFB1 Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc-hIFITM3
Plasmid#58461PurposeExpresses human IFITM3 with an N-terminal myc tag in mammalian cells.DepositorInsertIFITM3 (IFITM3 Human)
UseTagsmycExpressionMammalianMutationPromoterCMV IEAvailable sinceSept. 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
MP3
Plasmid#69650PurposeUntargetted Bacterial MutagenesisDepositorInsertdnaQ926 dam
UseTagsExpressionBacterialMutationPromoterE. coli PBADAvailable sinceSept. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-cBEST
Plasmid#125689PurposeC to T base editor for actinomycetesDepositorInsertstreptomyces codon optimized spCas9n (D10A), modified sgRNA casette, streptomyces codon optimized rAPOBEC1
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterermE*/tipAAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
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MP5
Plasmid#69677PurposeUntargetted Bacterial MutagenesisDepositorInsertdnaQ926 dam seqA ugi CDA1
UseTagsExpressionBacterialMutationPromoterE. coli PBADAvailable sinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pST_119_LVL2 cam
Plasmid#179333PurposeNT-CRISPR plasmid for integration of multiple gRNAs.DepositorInsertPtac tfoX, Ptet cas9, PJ23106 acrIIA4, sfGFP dropout to be replaced with gRNAs
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
TFORF1635
Plasmid#141855PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertHOXC11 (HOXC11 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
PP7_mCherry
Plasmid#61763PurposeExpresses PP7 coat protein fused to mCherryDepositorInsertPP7 coat protein fused to mCherry
UseLentiviralTagsmCherryExpressionMammalianMutationPromoterUbc promoterAvailable sinceFeb. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV/myc/ER-αT9ib
Plasmid#110768PurposeExpresses ER intrabody against mouse and human TLR9DepositorInsertintrabody against mouse and human TLR9
UseTagsER retention signal (SEKDEL), ER signal peptide, …ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only