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Plasmid#204400PurposeA C4-HSL sensor for B. subtilis on a shuttle vector for B. subtilis and E. coli.DepositorInsertsUseSynthetic BiologyTagsExpressionMutationPromoterPftsH and Synthetic promoter PRhl0L0L12Available sinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only
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pAC-GGPPipi
Plasmid#53280PurposeContains crtE and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and thereby produces geranylgeranyl diphosphate in E. coliDepositorInsertcrtE, idi
UseLow copy number bacterial cloning vectorTagsExpressionMutationPromoterendogenous promotersAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCFJ1272
Plasmid#44486DepositorInsertMinimal Mos1, MCS
UseMultiple cloning site vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
p5E-6xgata2aECEbasp
Plasmid#132968Purposegata2a endothelial enhancer (x6) and basal promoter in p5E Gateway vectorDepositorInsertgata2a endothelial enhancer (x6) with a b-actin basal promoter
UseGateway vectorTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUb-HisUb-Ubc9-ChE1 v4.5
Plasmid#185756PurposeBacterial expression of His-Ub, Ubc9 and ChE1 v4.5DepositorInsertsHis-Ub
Ubc9
ChE1 v4.5
UseTagsHis6ExpressionMutationK14RPromoterT7Available sinceAug. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
Cag.Luc.puro
Plasmid#74409PurposeConstitutive Luciferase expression in mammalian cells along with puromycin selectionDepositorInsertLuciferase
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceApril 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEX.d14GFP.sEF1a.Kozak.HA.NLS.Sce.T2A.BFP
Plasmid#45564PurposeExpresses I-Sce I nuclease and BFP tracker in mammalian cells, codes for eGFP donor sequenceDepositorInsertsd14 eGFP donor
I-Sce I Nuclease
BFP
UseTagsHAExpressionMammalianMutationdeleted n' 15 amino acidsPromoterAvailable sinceNov. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pME-H2B-mCerulean3
Plasmid#75156PurposeNuclear mCerulean2 (blue) fluorophore for use with 5’ promoters.DepositorInsertH2B-mCerulean3
UseMiddle entry vector for multisite gateway three f…TagsExpressionMutationPromotern/aAvailable sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
dCas9-CRY2Clust
Plasmid#200969Purposeinduction of phase-separated condensate by CRY2ClustDepositorInsertCRY2Clust
UseLentiviralTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
px330 VRER
Plasmid#101729PurposeExpresses a sgRNA and a Cas9 VRER variant that recognizes "NGCG" PAM motifsDepositorInsertSpCas9 VRER
UseTags3xFLAG-NLS and NLSExpressionMammalianMutationVRER (D1135V, G1218R, R1335E, and T1337R mutation…PromoterAvailable sinceOct. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGilesSHdI_redgreen_2395A_His6_triple red_pLeft1806_His6_double green
Plasmid#170048PurposeTri-Responsive Reporter Gene Construct for redgreen, triple red, and double greenDepositorInsertsmScarlet
COmScarlet
mRuby3
mWasabi dN
mWasabi CO
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNAS1B
Plasmid#61968PurposeDuet vector for co-expression of GFP and mCherry from PBAD and PLtetO promoters, respectively; parent vector for split mCherry assembly vectorsDepositorInsertsmCherry
GFP
UseTagsExpressionBacterialMutationSerine 65 to Glycine (GGT), Serine 72 to Alanine …PromoterPBAD and PLtetOAvailable sinceJan. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
Hygro-iDEST
Plasmid#75339PurposeGATEWAY destination vector for site-specific integration into the human AAVS1/PPP1R12C locus for doxycycline-inducible gene over-expressionDepositorInsertTRE-DEST
UseTagsExpressionMammalianMutationPromoterTRE-tightAvailable sinceMay 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
rtTA and TagBFP cassettes
Plasmid#62452PurposeMXS_chaining vector with 2 cassettesDepositorInsertsCMV::NLS-TagBFPx3-bGHpA
CMV::rtTA3-bGHpA
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS415_pGal-nCas9(H840A)
Plasmid#79616PurposeExpresses nCas9(H840A) in yeast cellsDepositorInsertSpCas9
UseTagsExpressionYeastMutationH840A for nickase Cas9PromoterpGal1Available sinceSept. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDD317 (Pmyo-2::GFP + SEC)
Plasmid#73344PurposeSEC vector carry Pmyo-2::GFP and ccdB sites for cloning homology armsDepositorInsertPmyo-2::GFP + SEC
UseCRISPR and Cre/LoxTagsGFPExpressionWormMutationPromotermyo-2Available sinceMarch 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pTD103-ARG1
Plasmid#106475PurposeExpresses acoustic reporter genes or gas vesicles (ARG1) in Salmonella typhimuriumDepositorInsertARG1
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpLuxAvailable sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFPL-Cg
Plasmid#61652PurposeGateway vector for generating cyan fluorescent protein fusions in fungiDepositorInsertenhanced cyan fluorescent protein
UseTagsExpressionPlantMutationPromoterAvailable sinceMarch 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-myrSNAP
Plasmid#60098PurposeExpresses membrane-targeted SNAP(f)-tag and histone-GFP, non-conditionalDepositorInsertsSNAP-tag
histone-GFP
UseAAV and Mouse TargetingTagsExpressionMammalianMutationPromoterAvailable sinceMarch 26, 2015AvailabilityAcademic Institutions and Nonprofits only