-
Plasmid#96848PurposeFor expression of B2AR-APEX in mammalian cellsDepositorInsertB2AR-APEX (ADRB2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
ABE8.17-d
Plasmid#136299Purposeexpresses ABE8.17-d in mammalian cellsDepositorInsertABE8.20-d
UseTagsC-terminal BPNLSExpressionMammalianMutationD10A in S. pyogenes Cas9, TadA mutations describe…PromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC6A2
Plasmid#131891PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC6A2 (SLC6A2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 9, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDF0430 huDisCas7-11-S1006-GGGS-D1221-U6-pro-Gluc
Plasmid#186987PurposeAAV transgene plasmid for Cas7-11-S1006-GGGS-D1221, with U6 promoter-driven expression of Gluc crRNA guideDepositorInsertcas7-11-s1006-gggs-d122, Gluc crRNA guide
UseAAVTagsExpressionMutations1006-gggs-d1221 deletionPromoterAvailable sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFastBac HTB DPP9S S730A
Plasmid#169946PurposePurification of catalytically-dead DPP9S from insect cellsDepositorInsertDPP9 (DPP9 Human)
UseTagsHIS-TEVExpressionInsectMutationS730APromoterpolyhedrinAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1 p37
Plasmid#169015PurposeExpresses GST-p37 (human, codon optimized for expression in bacteria)DepositorInsertp37 (UBXN2B Human)
UseTagsGSTExpressionBacterialMutationsequence was codon optimized for expression in E.…PromotertacAvailable sinceJune 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-Nsp5-HF
Plasmid#157687Purposemammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp5 under control of a tetracycline-inducible promoterDepositorInsertNsp5-HF (ORF1ab SARS-CoV-2)
UseTagsHis8-FlagExpressionMammalianMutationcodon optimizedPromoterAvailable sinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA5-FRT-TO-FH-Nsp2
Plasmid#157683Purposemammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp2 under control of a tetracycline-inducible promoterDepositorInsertFH-Nsp2 (ORF1ab SARS-CoV-2)
UseTagsFlag-His8ExpressionMammalianMutationcodon optimizedPromoterAvailable sinceAug. 4, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA-Flag1-3HABeta2-mEos2
Plasmid#114187Purposestudying clustering of Beta2 receptors by superresolution microscopy (PALM)DepositorInsertAdrenergic receptor beta 2 (ADRB2 Human)
UseTags3HA, FLAG, and mEOS2ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCS2-FLAG-hEcto/Tif1g-CAmut
Plasmid#20903DepositorInsertEctodermin (TRIM33 Human)
UseTagsFLAGExpressionMammalianMutationPoint mutagenesis of C125 and C128 to Alanine (RI…PromoterAvailable sinceMay 15, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-Nsp16-HF
Plasmid#157725Purposemammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp16 under control of a tetracycline-inducible promoterDepositorInsertNsp16-HF (ORF1ab SARS-CoV-2)
UseTagsHis8-FlagExpressionMammalianMutationcodon optimizedPromoterAvailable sinceAug. 6, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
ABE8.13-m
Plasmid#136296Purposeexpresses ABE8.13-m in mammalian cellsDepositorInsertABE8.13-m
UseTagsC-terminal BPNLSExpressionMammalianMutationD10A in S. pyogenes Cas9, TadA mutations describe…PromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBabe puro-HA-PIK3CA(H1047R;G320E)
Plasmid#33254DepositorInsertPIK3CA (PIK3CA Human)
UseRetroviralTagsHemagglutinin (HA)ExpressionMammalianMutationchanged histidine 1047 to arginine and glycine 32…PromoterSV40Available sinceJan. 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
Venus-G protein Beta2 in pcDNA3.1
Plasmid#42182DepositorInsertG protein Beta 2 (GNB2 Human)
UseTagsVenus(156-239)ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCDNA4TO-3XFLAG Halo K164A/H165A nsp1
Plasmid#175421PurposeExpresses K164A/H165A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.DepositorInsertCoV2 nsp1
UseTags3XFLAG HaloExpressionMammalianMutationPromoterCMVAvailable sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFastBac HTB DPP9S D808N
Plasmid#169947PurposePurification of catalytically-dead DPP9S from insect cellsDepositorInsertDPP9 (DPP9 Human)
UseTagsHIS-TEVExpressionInsectMutationD808NPromoterpolyhedrinAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mGnptg
Plasmid#78106PurposeMammalian expression of mouse Gnptg with C-terminal His tagDepositorInsertGnptg (Gnptg Mouse)
UseTagsHisExpressionMammalianMutationPromoterCMVAvailable sinceMay 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1-pDUXChd:hDUX4ctd-ca
Plasmid#198237PurposeLentivector with tet-inducible porcine DUXC/human DUX4 chimera (codon altered)DepositorInsertDUX4L pDUXChd:hDUX4ctd (codon altered) (DUX4 S. scrofa (porcine), Human)
UseLentiviralTagsExpressionMammalianMutationcodon alteredPromoterTREAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-FH-Nsp13
Plasmid#157713Purposemammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp13 under control of a tetracycline-inducible promoterDepositorInsertFH-Nsp13 (ORF1ab SARS-CoV-2)
UseTagsFlag-His8ExpressionMammalianMutationcodon optimizedPromoterAvailable sinceAug. 6, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA 3.2/V5-DEST SNAP29-V5
Plasmid#69821PurposeExpresses SNAP29-V5 in mammalian cellsDepositorInsertSynaptosomal-associated protein 29 (SNAP29 Human)
UseRetroviralTagsV5ExpressionMammalianMutationPromotercmvAvailable sinceApril 26, 2016AvailabilityAcademic Institutions and Nonprofits only