Addgene

We narrowed to 24,661 results for: cat

Showing: 11841 - 11860 of 24661 results

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NC3 pCDNA3.1 FLAG WTX (1-804)

Plasmid

#36958

Depositor

Randall Moon

Article

Major et al Science. 2007 May 18;316(5827):1043-6.

Insert

WTX (AMER1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 24, 2012

Availability

Academic Institutions and Nonprofits only
pET28a-his-Gluc-iLID

Plasmid

#172096

Purpose

Expresses the Gluc-iLID fusion protein in bacteria

Depositor

Avi Schroeder

Article

Adir et al Nat Commun. 2022 Apr 28;13(1):2328. doi:

Insert

Gaussia Luciferase - iLID

Use

Tags

Expression

Bacterial

Mutation

Gluc Mutations - M43L, M110L

Promoter

T7

Available since

April 29, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3 c-SRC (250-536) K298M HA

Plasmid

#42210

Depositor

Robert Lefkowitz

Article

Miller et al J Biol Chem. 2000 Apr 14;275(15):11312-9

Insert

c-SRC (250-536) HA (SRC Human)

Use

Tags

HA

Expression

Mammalian

Mutation

contains amino acids 250-536; K298M

Promoter

CMV

Available since

Jan. 28, 2013

Availability

Academic Institutions and Nonprofits only
NSP6-Flag

Plasmid

#210345

Purpose

Expresses SARS-CoV-2 NSP6 fused with Flag in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

SARS-CoV-2 NSP6 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1, Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Many synonymous changes due to codon optimization

Promoter

Available since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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NSP4-WT-Flag

Plasmid

#210339

Purpose

Expresses SARS-CoV-2 NSP4 fused with Flag in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

SARS-CoV-2 NSP4 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1, Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Many synonymous changes due to codon optimization

Promoter

Available since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pJS699

Plasmid

#168779

Purpose

Encodes the SARS-CoV-2 Spike Receptor Binding Domain (RBD) and can be combined with pJS697 through homologous recombination

Depositor

Timothy Whitehead

Article

Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:

Insert

Spike RBD (S betacoronavirus)

Use

Tags

Cmyc

Expression

Mutation

N343Q

Promoter

GAL1

Available since

Aug. 31, 2021

Availability

Academic Institutions and Nonprofits only

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pET-KIX (586-672)

Plasmid

#99336

Purpose

Bacterial expression of mouse CBP KIX domain

Depositor

Peter Wright

Article

Radhakrishnan et al J Mol Biol. 1999 Apr 16;287(5):859-65.

Insert

CBP KIX (586-672) (Crebbp Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 6, 2017

Availability

Academic Institutions and Nonprofits only

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MAC-TMPRSS2

Plasmid

#158385

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

TMPRSS2 (TMPRSS2 Human)

Use

Tags

MAC-tag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 26, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pBabe puro C-Raf 22W

Plasmid

#12593

Depositor

Channing Der

Article

McFall et al Mol Cell Biol. 2001 Aug . 21(16):5488-99

Insert

N-term truncated mut of Raf-1 (RAF1 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

22W, N-terminally truncated

Promoter

Available since

Sept. 21, 2006

Availability

Academic Institutions and Nonprofits only
pFRLuc-CoV2-delStem1

Plasmid

#177616

Purpose

Dual luciferase reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting. 3-nucleotide deletion in Stem 1 (negative control).

Depositor

Junjie Guo

Article

Sun et al Proc Natl Acad Sci U S A. 2021 Jun 29;11

Insert

SARS-CoV-2 FSE

Use

Luciferase

Tags

Expression

Mammalian

Mutation

3-nucleotide (UAC) deletion in Stem 1

Promoter

Available since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-Nsp10-HF

Plasmid

#157708

Purpose

mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp10 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp10-HF (ORF1ab SARS-CoV-2)

Use

Tags

His8-Flag

Expression

Mammalian

Mutation

codon optimized

Promoter

Available since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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p7126 pHAGE-P-CMVt-N-HA-GAW-FTH1

Plasmid

#100152

Purpose

Expresses FTH1

Depositor

Peter Howley

Article

Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

Insert

FTH1 (FTH1 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 23, 2018

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-V5-Lem2

Plasmid

#120245

Purpose

Expresses V5-tagged Lem2 in lentiviral vector

Depositor

Larry Gerace

Article

Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

Insert

Lem2 (Lemd2 Mouse)

Use

Lentiviral

Tags

V5

Expression

Mutation

Promoter

EF1a

Available since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pGIPz-PD-L1 4NQ

Plasmid

#121487

Purpose

Expression of human PD-L1 4NQ with PD-L1 shRNA

Depositor

Mien-Chie Hung

Article

Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

Inserts

PD-L1 4NQ
hPD-L1 shRNA

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

N35Q, N192Q, N200Q and N219Q

Promoter

CMV

Available since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3 c-SRC (250-536)

Plasmid

#42208

Depositor

Robert Lefkowitz

Article

Miller et al J Biol Chem. 2000 Apr 14;275(15):11312-9

Insert

c-SRC (250-536) (SRC Human)

Use

Tags

Expression

Mammalian

Mutation

contains amino acids 250-536

Promoter

CMV

Available since

Feb. 5, 2013

Availability

Academic Institutions and Nonprofits only
p7052 pHAGE-P-CMVt-N-HA-GAW-AURKB

Plasmid

#100142

Purpose

Expreses AURKB

Depositor

Peter Howley

Article

Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

Insert

AURKB (AURKB Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-FH-Nsp12

Plasmid

#157710

Purpose

mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp12 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

FH-Nsp12 (ORF1ab SARS-CoV-2)

Use

Tags

Flag-His8

Expression

Mammalian

Mutation

codon optimized

Promoter

Available since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pORANGE Shank2-GFP KI

Plasmid

#131496

Purpose

Endogenous tagging of Shank2: C-terminal (amino acid position: STOP codon)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Shank2, Shank2 Rat, Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6 and Cbh

Available since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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F-MED26

Plasmid

#15367

Depositor

Ronald Conaway, Joan Conaway

Article

Sato et al Mol Cell. 2004 Jun 4. 14(5):685-91.

Insert

MED26 (MED26 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

N/A

Promoter

Available since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pPICZ-aC-KHC-BZ

Plasmid

#160427

Purpose

Expression of c-luciferase from sp. Kornikeria hastingsi carriebowae (Belize)

Depositor

Todd Oakley

Article

Hensley et al Mol Ecol. 2020 Oct 8. doi: 10.1111/mec.1

Insert

luciferase

Use

Luciferase

Tags

6x Histidine tag, Yeast alpha secretion factor, c…

Expression

Yeast

Mutation

Promoter

AOX

Available since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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