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CRISPR-Cas12a genome editing in E. coli. Contains a constitutively CRISPR array targeting the lacZ gene in E. coli genome and donor DNA to insert an constitutively expressed rfp reporter gene.
Entry clone expressing gRNA. Target site can be introduced between tRNA and gRNA backbone. For use in plants and compatible with the MultiSite Gateway system
Kanamycin and Chloramphenicol resistant; destination vector engineered to integrate into the mycobacteriophage att-L5 site and designed to be used with the multisite Gateway cloning system