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Addgene
Showing: 11781 - 11800 of 20581 results

  4. pCASCADE-gltA2-udhA

    Plasmid
    #65819
    Purpose
    gltA-udhA targeting guide RNA array E. coli , Low Phosphate Induction
    Depositor
    Michael Lynch
    Insert
    gltA2-udhA guide array
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    E . coli ugpB gene promoter, low phosphate induc…
    Availability
    Academic Institutions and Nonprofits only
    65819-plasmid-map-sequence-id-111979

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  5. pTJK637

    Plasmid
    #138010
    Purpose
    GATA1 knockout
    Depositor
    Tami Kingsbury
    Insert
    GATA1 sgRNA (GATA1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    None
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    138010-plasmid-map-sequence-id-275012

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  8. pCas9CR4-VQR

    Plasmid
    #120224
    Purpose
    pCas9CR4 with VQR mutations for changing PAM site specificity to NGAN or NGNG from Kleinstiver et al. 2015
    Depositor
    Christopher Reisch
    Insert
    SpCas9-VQR
    Use
    Synthetic Biology
    Tags
    ssrA
    Expression
    Mutation
    Mutations D1135V/R1335V/T1337R
    Promoter
    pTet
    Availability
    Academic Institutions and Nonprofits only
    120224-plasmid-map-sequence-id-233296

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  9. pCas9CR4-NG

    Plasmid
    #120225
    Purpose
    pCas9CR4 with mutations to change PAM site specificity to NG from Nishimasu et al.
    Depositor
    Christopher Reisch
    Insert
    SpCas9-NG
    Use
    Synthetic Biology
    Tags
    ssrA
    Expression
    Mutation
    Mutations R1335V/L1111R/D1135V/G1218R/E1219F/A132…
    Promoter
    pTet
    Availability
    Academic Institutions and Nonprofits only
    120225-plasmid-map-sequence-id-233264

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  10. pCas9CR5-NG

    Plasmid
    #120228
    Purpose
    pCas9CR5 with mutations to change PAM site specificity to NG from Nishimasu et al.
    Depositor
    Christopher Reisch
    Insert
    SpCas9-NG
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Mutations R1335V/L1111R/D1135V/G1218R/E1219F/A132…
    Promoter
    pTet
    Availability
    Academic Institutions and Nonprofits only
    120228-plasmid-map-sequence-id-233263

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  11. pNSU299_TDH3

    Plasmid
    #166084
    Purpose
    Plasmid for constituive spCas9 expression and tet-inducible expression of sgRNA binding to the promoter of TDH3 for double stranded break formation in yeast.
    Depositor
    Rebecca Burgess
    Insert
    Promoter of TDH3 (TDH3 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Availability
    Academic Institutions and Nonprofits only
    166084-plasmid-map-sequence-id-329123

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  12. pNSU299_CUP1-1

    Plasmid
    #166086
    Purpose
    Plasmid for constituive spCas9 and tet-inducible CUP1-1 targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Rebecca Burgess
    Insert
    CUP1-1 (CUP1-1 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Availability
    Academic Institutions and Nonprofits only
    166086-plasmid-map-sequence-id-330352

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  13. ipUSEPR-sg-Hs-CDK1

    Plasmid
    #188684
    Purpose
    control sgRNA
    Depositor
    Lewis Cantley
    Insert
    sgCDK1 (CDK1 Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    WT
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    188684-plasmid-map-sequence-id-375070

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  14. ipUSEPR-sg-Hs-KIF11

    Plasmid
    #188685
    Purpose
    control sgRNA
    Depositor
    Lewis Cantley
    Insert
    sgKIF11 (KIF11 Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    WT
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    188685-plasmid-map-sequence-id-375124

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  16. pEGB3_alpha1 gRNAs 2.1 P35S -104 -162 -229 (GB2759)

    Plasmid
    #160631
    Purpose
    Multiplex construct with 2.1 aptamer in scf to target -104 -162 -229 positions of 35s promoter.
    Depositor
    Diego Orzaez
    Insert
    gRNA
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    U626
    Availability
    Academic Institutions and Nonprofits only
    160631-plasmid-map-sequence-id-321967

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  17. 1167A

    Plasmid
    #218233
    Purpose
    HDR plasmid for inserting Ceratitis capitata transformer female intron into the white pupae gene
    Depositor
    Omar Akbari
    Insert
    putative metabolite transport protein
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    218233-plasmid-map-sequence-id-433264

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  18. miniTurbo-dCasRx

    Plasmid
    #219821
    Purpose
    EF1α-driven expression of miniTurbo fused to dCasRx with GFP (Adapted from plasmid #153209)
    Depositor
    Philipp Maass
    Insert
    dCasRx
    Use
    CRISPR
    Tags
    miniTurbo
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a and hU6
    Availability
    Academic Institutions and Nonprofits only
    219821-plasmid-map-sequence-id-440859

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  19. dCas9-miniTurbo

    Plasmid
    #219822
    Purpose
    EF1α-driven expression of dCas9 fused to miniTurbo with GFP (Adapted from plasmid #153209)
    Depositor
    Philipp Maass
    Insert
    dCas9
    Use
    CRISPR
    Tags
    miniTurbo
    Expression
    Mammalian
    Mutation
    D10A/H841A
    Promoter
    EF1a
    Availability
    Academic Institutions and Nonprofits only
    219822-plasmid-map-sequence-id-440858

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  20. pCFD3-dUG-DH1

    Plasmid
    #138963
    Purpose
    Used to edit the endogenous Drosophila kkv gene to encode a fluorescent Chitin Synthase.
    Depositor
    Paul Adler
    Article
    Adler et al bioRxiv 2020
    Insert
    DH1 oligo
    Use
    CRISPR
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    138963-plasmid-map-sequence-id-274780

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Showing: 11781 - 11800 of 20581 results