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Plasmid#157650PurposeGolden Gate assembly of a pET28-compatible fusion-protein ORF insertDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoternoneAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only
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GGDest3-Amp
Plasmid#157651PurposeGolden Gate assembly of a pTXB1 compatible fusion-protein ORF insertDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoternoneAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPtGG-1
Plasmid#89990PurposepBR322-based entry vector for Gateway cloningDepositorTypeEmpty backboneUseGolden gate entry vectorTagsExpressionMutationPromoterAvailable sinceJune 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJJB1561
Plasmid#218626PurposeExpress geraniol synthase (GES), Idi1, and Erg20(F96W, N127W) all with ePTS1 tags and cytosolic Erg20_WT_synthetic homodimerDepositorInsertGES
UseTagsePTS1ExpressionYeastMutationPromoterAvailable sinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJJB1439
Plasmid#218589PurposeExpress peroxisomal Idi1p, Erg20(F96W,N127W)p, and GESp to convert IPP to geraniolDepositorInsertGES
UseTagsePTS1ExpressionYeastMutationErg20(F96W, N127W)PromoterAvailable sinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJJB170
Plasmid#218591PurposeExpress engineered TFs Adr1c(S230A), Pip2c, and Oaf1cDepositorInsertAdr1
UseTagsExpressionYeastMutationAdr1(S230A), Pip2(1-168 + 2,497-2,988), Oaf1(1-30…PromoterAvailable sinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSKT001
Plasmid#218594PurposeExpress degron assay: Degradation tag-YFP-ePTS1DepositorInsertYFP
UseTagsUbiY degradation tag and ePTS1ExpressionYeastMutationPromoterAvailable sinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
L1T18 - T2PsB/p35S preswitched (mTagBFP2, mCherry, NeonGreen)
Plasmid#219690PurposeLevel 1 preswitched History-dependent target, switch with Bxb1, p35S promoter, and output: mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance).DepositorInsertT2PsB/p35S (mTagBFP2, mCherry, NeonGreen)
UseTagsExpressionPlantMutationPromoterAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
L1T11 - T2BP/p35S (BFP, mCherry, NeonGreen)
Plasmid#219688PurposeLevel 1 History-dependent target, switch Bxb1 then PhiC31, p35S promoter, and output: mtagBFP2 then mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance).DepositorInsertT2BP/p35S (BFP, mCherry, NeonGreen)
UseTagsExpressionPlantMutationPromoterAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
L1T10 - T2PB/pUBQ10 (mTagBFP2, mCherry, NeonGreen)
Plasmid#219687PurposeLevel 1 History-dependent target, switch PhiC31 then Bxb1, pUBQ10 promoter, and output: mtagBFP2 then mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance).DepositorInsertT2PB/pUBQ10 (mTagBFP2, mCherry, NeonGreen)
UseTagsExpressionPlantMutationPromoterAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
L1T9 - T2PB/p35S (mTagBFP2, mCherry, NeonGreen)
Plasmid#219686PurposeLevel 1 History-dependent target, switch PhiC31 then Bxb1, p35S promoter, and output: mtagBFP2 then mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance).DepositorInsertT2PB/p35S (mTagBFP2, mCherry, NeonGreen)
UseTagsExpressionPlantMutationPromoterAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
MUT_S1-H1
Plasmid#218394Purpose3-exon minigene, with SRSF1 motif-rich middle exon, hnRNPA1 motif-rich region in second intron, and AG>GG mutation in final 3' splice siteDepositorInsertModified CHO DHFR minigene
UseTagsExpressionMammalianMutationPromotertetracycline-responsive promoterAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
MUT_S7-H1
Plasmid#218396Purpose3-exon minigene, with SRSF7 motif-rich middle exon, hnRNPA1 motif-rich region in second intron, and AG>GG mutation in final 3' splice siteDepositorInsertModified CHO DHFR minigene
UseTagsExpressionMammalianMutationPromotertetracycline-responsive promoterAvailable sinceMay 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
GGDest1-Amp
Plasmid#157649PurposeGolden Gate assembly of a pcDNA3.1+ or BioBrick RFC23 compatible fusion-protein ORF insertDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoternoneAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAGImC_V5-CSNK1A1
Plasmid#92014PurposeExpresses V5-CSNK1A1, includes IRES-mCherryDepositorInsertCSNK1A1 (CSNK1A1 Human)
UseTagsV5ExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
IBMc233
Plasmid#161939PurposeCarries Golden Gate substitution insert for IBM with M86 intein. PPhlF as C-lobe promoterDepositorInsertpSB1A3-BbsI-M86N-T-cat-PPhlF-RiboJ-B32-M86C-SapI
UseTagsExpressionBacterialMutationPromoterPPhlFAvailable sinceMarch 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
IBMc050
Plasmid#161938PurposeCarries Golden Gate substitution insert for IBM with M86 intein. Plux2 as C-lobe promoterDepositorInsertpSB1A3-BbsI-M86N-cat-Plux2-B32-M86C-SapI
UseTagsExpressionBacterialMutationPromoterPlux2Available sinceMarch 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
IBMc318
Plasmid#161941PurposeCarries Golden Gate substitution insert for ER-LBD domain-insertion mappingDepositorInsertpSB1A3-BbsI-G-ER-LBD-S-SapI
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAGM4723:Ble:Cas9
Plasmid#112208PurposeGolden Gate Level 2 construct encoding Cas9-YFP, Zeocin resistance.DepositorInsertsCas9
SheBle
UseCRISPR; Diatom, phaeodactylumTagsPhaeodactylum fcp promoter and terminator and YFPExpressionMutationPhaeodactylum Fcp promoterPromoterPhaeodactylum FcpAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
MUT_S1-spacer-H1
Plasmid#218399Purpose3-exon minigene, with SRSF1 motif-rich middle exon, hnRNPA1 motif-rich region in second intron following a neutral spacer, and AG>GG mutation in final 3' splice siteDepositorInsertModified CHO DHFR minigene
UseTagsExpressionMammalianMutationPromotertetracycline-responsive promoterAvailable sinceJune 17, 2024AvailabilityAcademic Institutions and Nonprofits only