Addgene

We narrowed to 3,036 results for: aaas

Showing: 1161 - 1180 of 3036 results

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mCherry-FKBP-SAC1-HLx10

Plasmid

#108137

Purpose

Recruitable SAC1 with extended linker

Depositor

Gerry Hammond

Article

Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

Insert

mCherry:FKBP1A(3-108):[GGSA]4GG:SACM1L(1-520):[EAAAR]10:SACM1L(521-587) (SACM1L Human)

Use

Tags

Expression

Mammalian

Mutation

SACM1L(1-520):[EAAAR]10:SACM1L(521-587)

Promoter

CMV

Available since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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-962 human cyclin D1 promoter TCF (0)-(4) sites mutant pGL3Basic

Plasmid

#32737

Depositor

Frank McCormick

Article

Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

Insert

CCND1 promoter (CCND1 Human)

Use

Luciferase

Tags

Expression

Mutation

-539 TCF(0) ATGAAAG deletion; -75 TCF(1) and -68 …

Promoter

-962 CCND1

Available since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only
pENTR/U6 HDAC4 shRNA

Plasmid

#32220

Depositor

Reuben Shaw

Article

Mihaylova et al Cell. 2011 May 13;145(4):607-21.

Insert

shRNA against murine HDAC4 (Hdac4 Mouse)

Use

RNAi; Gateway u6 entry vector

Tags

Expression

Mutation

Promoter

human U6

Available since

Sept. 16, 2011

Availability

Academic Institutions and Nonprofits only
PX458_ARID5B_1

Plasmid

#86350

Purpose

Encodes gRNA for 3' target of human ARID5B

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against ARID5B (ARID5B Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pW301-lenti-spsgRNA-hsITGB1-pEF1s-NLS-mNeonGreen-P2A-BlastR

Plasmid

#170817

Purpose

Lentiviral vector to co-express a human ITGB1 spsgRNA with NLS-mScarlet-I

Depositor

Kenneth Yamada

Article

Wang et al Cell. 2021 Jul 8;184(14):3702-3716.e30.

Insert

NLS-mNeonGreen-P2A-BlastR

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

NA

Promoter

Available since

June 8, 2021

Availability

Academic Institutions and Nonprofits only

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pSECB sgMTHFD2L_2

Plasmid

#106307

Purpose

Express Cas9 and sgRNA targeting MTHFD2L

Depositor

Richard Possemato

Article

Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

Insert

sgRNA targeting MTHFD2L (MTHFD2L Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 27, 2018

Availability

Academic Institutions and Nonprofits only

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PX458_CEBPG_1

Plasmid

#86344

Purpose

Encodes gRNA for 3' target of human CEBPG

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against CEBPG (CEBPG Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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GFP-PRMT9 L182A/D183A/I184A/G185A

Plasmid

#79676

Purpose

mammalian expression of GFP-PRMT9 catalytically inactive LDIG(182-185)AAAA mutant

Depositor

Steven Clarke

Article

Hadjikyriacou et al J Biol Chem. 2015 May 15. pii: jbc.M115.

Insert

PRMT9 (PRMT9 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

L182A/D183A/I184A/G185A catalytic mutant

Promoter

CMV

Available since

June 27, 2016

Availability

Academic Institutions and Nonprofits only
mCherry-FKBP-SAC1C389S-HLx8

Plasmid

#108138

Purpose

Recruitable inactive SAC1 with extended linker

Depositor

Gerry Hammond

Article

Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

Insert

mCherry:FKBP1A(3-108):[GGSA]4GG:SACM1LC389S(1-520):[EAAAR]8:SACM1L(521-587) (SACM1L Human)

Use

Tags

Expression

Mammalian

Mutation

SACM1LC389S(1-520):[EAAAR]8:SACM1L(521-587)

Promoter

CMV

Available since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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pCRII-Topo U6-SpAi9R-U6-SpDMDR

Plasmid

#78609

Purpose

Expresses gRNAs (for SpCas9) targeting 3’ of Ai9 stop cassette and Dmd intron 23

Depositor

Amy Wagers

Article

Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

Insert

U6-SpAi9R-U6-SpDMDR

Use

Unspecified

Tags

Expression

Mutation

Promoter

U6

Available since

May 19, 2021

Availability

Academic Institutions and Nonprofits only
PX458_CEBPG_2

Plasmid

#86345

Purpose

Encodes gRNA for 3' target of human CEBPG

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against CEBPG (CEBPG Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pAAVsc.U6-sgGTTG.CMV/CB-Gluc

Plasmid

#121512

Purpose

Expresses sgRNA targeting the intron in the MADM alleles (GT and TG).

Depositor

Guangping Gao

Article

Wang et al Nat Biotechnol. 2018 Oct;36(9):839-842.

Insert

sgGTTG

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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PSMD14 E5.4 gRNA

Plasmid

#90858

Purpose

3rd generation lentiviral gRNA plasmid targeting human PSMD14

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

PSMD14 (Guide Designation E5.4)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

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CBX1 A3.5 gRNA

Plasmid

#90566

Purpose

3rd generation lentiviral gRNA plasmid targeting human CBX1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

CBX1 (Guide Designation A3.5)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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MSCV-PM BRD7 sh2

Plasmid

#41928

Depositor

Stephen Elledge

Article

Burrows et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

Insert

BRD7 (BRD7 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 10, 2013

Availability

Academic Institutions and Nonprofits only
AA244

Plasmid

#215945

Purpose

Fragmid fragment: (guide cassette) guide expression; positive control cell surface

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

U6_v1; sgCD59_v1 [Sp]; trRNA_v4 [Sp]

Use

CRISPR; Fragment

Tags

Expression

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pRDB_131

Plasmid

#216095

Purpose

Cas12a [EnAs] CRISPRa targeting CD97, CD4, CD26, CD274, positive control

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

CD97, CD4, CD26, CD274 guides

Use

CRISPR and Lentiviral; Positive controls

Tags

Expression

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-v2-ALDH1A3gRNA1

Plasmid

#189746

Purpose

The construct was used for expression of gRNA and Cas9 for knocking out of the ALDH1A3 gene.

Depositor

Robert Sobol

Article

Li et al Commun Biol. 2021 Dec 21;4(1):1420. doi:

Insert

Cas9, ALDH1A3 gRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1-Tet-Puro-shMMERVK10c_1

Plasmid

#185020

Purpose

shRNA mediated knockdown

Depositor

Denes Hnisz

Article

Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

Insert

ERV_MMERVK10c

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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px335-sgRNA-EF(Cas9n)-mHdac1-R2

Plasmid

#122308

Purpose

Expresses sgRNA targeting mouse Hdac1 and Cas9 nickase in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Hdac1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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