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Plasmid#119862Purposeprecusor D-galactose/methyl-galactoside ABC transporter periplasmic binding protein, mglB, L244C, D287CDepositorInsertD-galactose/methyl-galactoside ABC transporter periplasmic binding protein, mglB, L244C, D287C (mglB Escherichia coli str. K-12)
UseTagsnoneExpressionBacterialMutationLeucine 244 to Cysteine, Aspartate 287 to CysteinePromoterT5Available sinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHAGE- TRE-DEST-CBioTAP
Plasmid#53569PurposeLentiviral destination vector for C-Bio-1xproteinA constructs using Gateway recombination System. Design for cDNA clones from the CCSBHuman Orfeome Collection.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
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His-Sin3a-115-212(pL01)
Plasmid#124386PurposeE.Coli produced SIN3A PAH1 purified by FPLC nickel columnDepositorInsertHis-Sin3a-115-212 (SIN3A )
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceMay 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
LZF70: Cre-off hSyn-QuasAr3-dark Citrine (FAS)
Plasmid#104117PurposesynOptopatch constructDepositorInsertQuasAr3-dark Citrine
UseLentiviralTagsExpressionMammalianMutationPromoterhuman synapsin IAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pWPI-TFDP1
Plasmid#114299PurposeConstitutive expression of the TFDP1 proteinDepositorInsertTFDP1 (TFDP1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLITMUS-rpoN-cI4A5C6G7G,P-J23106-geneIII
Plasmid#80864PurposePhagemid (encodes evolved cI4A5C6G7G,P and M13 gene III)DepositorInsertsM13 gene III
cI4A5C6G7G,P
UseTagsExpressionBacterialMutation238G to 238D (please see depositor comment below)PromoterBBa_J23106 and rpoNAvailable sinceDec. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
A3A-Gam
Plasmid#119769PurposeConversion of C to T monocotyledons protoplastsDepositorInsertGam-APOBEC3A-nCas9-NLS-2XUGI-NLS
UseTagsNLS (flanking UGI)ExpressionPlantMutationnCas9 has a mutation D10A in wild type SpCas9Promotermaize Ubiquitin-1Available sinceSept. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
mTFP1-Actin-7
Plasmid#55462PurposeLocalization: Actin, Excitation: 462, Emission: 492DepositorInsertActin (ACTB Human)
UseTagsmTFP1ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-IP3KC
Plasmid#32527DepositorInsertIP3KC (ITPKC Human)
UseTags6xHISExpressionMammalianMutationPromoterCMVAvailable sinceOct. 26, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMEH295 NLS dTomato 1x wild type MS2 loop
Plasmid#168214PurposeMammalian expression of nuclear-localized dTomato with 1 wild type MS2 loopDepositorInsertNLS dTomato
UseLentiviralTagsNLSExpressionMammalianMutationPromoterCMVAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLITMUS-rpoN-cI4A5C6G7G-J23106-geneIII
Plasmid#80909PurposePhagemid (encodes cI4A5C6G7G and M13 gene III)DepositorInsertsM13 gene III
cI4A5C6G7G
UseTagsExpressionBacterialMutation238G to 238D (please see depositor comment below)PromoterBBa_J23106 and rpoNAvailable sinceDec. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSLQ8454 pHR-hU6-LbCpf1 crRNA BB-EF1a-Puro-WPRE
Plasmid#140087PurposeBackbone for cloning U6 driven LbCpf1 (Cas12a) crRNA, with EF1a driven puromycin resistanceDepositorInsertLbCpf1 (LbCas12a) crRNA backbone
UseLentiviralTagsEF1a-PuroExpressionMammalianMutationPromoterU6Available sinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL-p27UTR
Plasmid#20881DepositorInsertp27 3'UTR (Cdkn1b Mouse)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-GSK3B_IDG-K
Plasmid#158165PurposeGateway destination clone of GSK3B (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertminiTurbo-V5-GSK3B (GSK3B Human)
UseLentiviral; Gateway destinationTagsminiTurbo-V5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceOct. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSG-3'Hu-RelA
Plasmid#27276DepositorInsertRelA (RELA Human)
UseTagsGAL4 (aa1-147)ExpressionMammalianMutationContains only the transactivation domain (aa 292-…PromoterAvailable sinceFeb. 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
pIS47 C20orf139 3'UTR
Plasmid#14489DepositorInsertC20orf139 3'UTR (SRXN1 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterAvailable sinceMarch 7, 2007AvailabilityAcademic Institutions and Nonprofits only -
rGSKmyc-pXT7
Plasmid#15492DepositorInsertGSK-3 beta (Gsk3b Rat)
UseXenopus rna expressionTagsmycExpressionMutationPromoterAvailable sinceAug. 1, 2007AvailabilityAcademic Institutions and Nonprofits only -
mKO-Golgi-7
Plasmid#57843PurposeLocalization: Golgi Complex, Excitation: 548, Emission: 561DepositorInsertGolgi (B4GALT1 Human)
UseTagsmKOExpressionMammalianMutationaa 1-82 of NM_001497.3PromoterCMVAvailable sinceOct. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRSV-p65T305A
Plasmid#106454PurposeNF-kB p65, kinase site 305 mutatedDepositorInsertNF-kB p65, kinase site 305 mutated (RELA Human)
UseTagsExpressionMammalianMutationkinase site inactivatedPromoterAvailable sinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only