-
Plasmid#105986PurposeTo make an auxin inducible degron mutantDepositorInsertT2A-Bsr
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEB2_mScarletI_mVenusNB-MrkH
Plasmid#182291Purposeexpresses c-di-GMP biosensor in E. coliDepositorInsertmVenusNB-MrkH
UseTagsmVenusNB internal fusion to MrkHExpressionBacterialMutationPromoterproCAvailable sinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLHDR02_EGFP_tCD8
Plasmid#124061PurposeConstruction of 1st donor for scarless genome editingDepositorInsertsEGFP
tCD8
UseMouse Targeting; Human targetingTagsExpressionMutationPromoterHuman UbC promoter modifiedAvailable sinceApril 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1-p19-T7-Vif
Plasmid#46313DepositorInsertsp19
Vif DNA-1
linker
Vif DNA-2
UsePro-sirnaTags6XHis and GSTExpressionBacterialMutationPromoterAvailable sinceJan. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
KG#121
Plasmid#110879PurposeExpresses the C. elegans unc-31 cDNA pan-neuronallyDepositorInsertsrab-3 promoter
unc-31 cDNA
unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
UseTagsExpressionBacterialMutationMissing first 408bpPromoterAvailable sinceJune 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-UBE2S-UBD
Plasmid#66713PurposeExpresses the UBE2S-UBD fusion protein in E. coliDepositorUseTagsGST-3CExpressionBacterialMutationPromoterT7Available sinceJuly 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTK194
Plasmid#59563PurposeTC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP XbaI attB1 Kozak mKate opal attB2 AscI (truncated E1) 3'UTR Poly A I-SceIDepositorInsertSGP XbaI attB1 Kozak mKate opal attB2 AscI
UseTagsExpressionMutationPromoterAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSJ2039 (pRS316-NOP1pr-GFP1-10-SCS2TM)
Plasmid#86418PurposeONM/ER split GFP1-10DepositorInsertNOP1pr-GFP1-10-Scs2TM
UseTagsGFP1-10ExpressionYeastMutationPromoterNOP1Available sinceFeb. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAEF5
Plasmid#136305PurposePlasmid encoding the Cas9 gene and a gRNA expression cassette allowing to clone a single or multiple gRNAs for DSBs induction in yeast. Constructed by Aubin FleissDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSilencer2.1-U6-T1-U6-AP3
Plasmid#190828PurposeFor mammalian expression of T1 and AP3, the RNA aptamers of ncOGT and GFP, respectively. Expression of each aptamer is driven by a U6 promoter.DepositorInsertsT1 (an RNA aptamer of OGT)
AP3 (an RNA aptamer of GFP)
UseAffinity Reagent/ Antibody and Synthetic BiologyTagsExpressionMammalianMutationPromoterU6Available sinceJan. 9, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
14UAS PSD95:GFP 5UAS DSRedExpress
Plasmid#74315PurposeEncodes a PSD95:GFP fusion protein and DsRedExpress from separate UAS cassettes; for long-term two-photon time-lapse imaging of dendrite growth and synaptogenesis in zebrafishDepositorInsertszebrafish PSD95
DsRedExpress
UseTagsEGFPExpressionMutationY599H and I793S (please see depositor comment bel…PromoterAvailable sinceMay 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
1137=Hsp70Bb-Cas9[dsRed]
Plasmid#149424PurposeThe piggyBac plasmid harboring Hsp70Bb-Cas9-T2A-eGFP-p10 and Opie2-dsRed-SV10 (marker gene)DepositorInsertCas9
UseCRISPRTagseGFPExpressionInsectMutationPromoterDmel Hsp70BbAvailable sinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
CAG:: mCherry-2a-hM4Dnrxn
Plasmid#52523PurposeCo-expresses hM4Dnrxn and a red fluorescent labelDepositorInsertmCherry-2a-hM4Dnrxn
UseTags2xHAExpressionMammalianMutationPromoterCAgAvailable sinceApril 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a_T5-ARG1
Plasmid#106476PurposeExpresses acoustic reporter genes or gas vesicles (ARG1) in Escherichia coli Nissle 1917DepositorInsertARG1
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT5Available sinceMarch 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_T7-ARG2
Plasmid#106474PurposeExpresses acoustic reporter genes or gas vesicles (ARG2) in Escherichia coliDepositorInsertARG2
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7Available sinceMarch 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
SBE-NLS-mCherry PGK-rtTA3
Plasmid#195332PurposeLentiviral construct of TGFβ reporter (nuclear mCherry) and hPGK driven rtTA3DepositorInsertsNLS-mCherry
rtTA3
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterhPGK and miniCMVAvailable sinceFeb. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_LifeAct-iBlueberry
Plasmid#73176PurposeExpressing iBlueberry and mCherry in mammalian cellsDepositorInsertLifeAct-iBlueberry
UseTagsExpressionMammalianMutationmutation E177Q in iBlueberryPromoterAvailable sinceApril 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLV-dfROX
Plasmid#137170PurposeLentiviral transfer vector for expression of roGFP2 and HyPer RedDepositorInsertroGFP2-2A-HyPer Red
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceApril 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCFJ1201
Plasmid#44483DepositorInsertMinimal Mos1, unc-119, peel-1, MCS
UseMultiple cloning site vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCK760
Plasmid#192643PurposeExpresses BBa_J23110-sfGFP (capacity monitor) and J3-Bba_J23117-mRFP (CRISPRa reporter) on pSC101-AmpRDepositorInsertBBa_J23110-sfGFP
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterBBa_J23110Available sinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only