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pWAP-HGF

Plasmid

#83503

Purpose

Generation of transgenic mice overexpressing the HGF under the control of the WAP gene promoter

Depositor

Lothar Hennighausen, Marta Gallego

Article

Gallego et al Oncogene. 2003 Nov 20;22(52):8498-508.

Insert

HGF (Hgf Mouse)

Use

Mouse Targeting

Tags

Expression

Mutation

also contains beta-globin sequences from pUC198

Promoter

mouse WAP (whey acidic protein)

Available since

Feb. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX-6p-2-EWSR1

Plasmid

#46384

Depositor

Heinz Gehring

Article

Pahlich et al Proteins. 2005 Oct 1;61(1):164-75.

Insert

EWSR1 (EWSR1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

July 29, 2013

Availability

Academic Institutions and Nonprofits only
pET23b-NTF2

Plasmid

#108918

Purpose

Expresses NTF2

Depositor

Larry Gerace

Article

Cassany et al Methods Mol Biol. 2009;464:181-205. doi:

Insert

NTF2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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OaAEP1_C247A

Plasmid

#200307

Purpose

Bacterial expression plasmids for production of C247A mutant of asparaginyl endopeptidase 1 from O. affinis (OaAEP1_C247A)

Depositor

Artur Krężel

Article

Antonenko et al Bioconjug Chem. 2023 Mar 15;34(4):719-27

Insert

Asparaginyl endopeptidase from Oldenlandia affinis

Use

Tags

Smt3

Expression

Bacterial

Mutation

Cysteine 247 to Alanine

Promoter

T7

Available since

June 1, 2023

Availability

Academic Institutions and Nonprofits only

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hTLR2 flag

Plasmid

#13082

Depositor

Ruslan Medzhitov

Insert

TLR2 (TLR2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Amino acids 1-16 have been replaced with mammalia…

Promoter

Available since

Oct. 3, 2006

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-LAMP-1-mGFP-IRES-Puromycin

Plasmid

#134868

Purpose

Expresses EGFP-tagged lysosomal marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Lysosomal-associated membrane protein 1 (LAMP1 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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pLeAPS-GFP-blast

Plasmid

#182230

Purpose

Lentiviral transfer plasmid for the LeAPS packaging system; encodes GFP-P2A-blasticidin transgene

Depositor

Michael Birnbaum

Article

Dobson et al Nat Methods. 2022 Apr;19(4):449-460. doi

Insert

GFP-P2A-Blasticidin

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pLX317-hcRED

Plasmid

#115440

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

hcRED

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-fDIO-mNeonGreen

Plasmid

#99133

Purpose

An AAV genome with Flp recombinase-dependent expression of mNeonGreen from the CAG promoter

Depositor

Viviana Gradinaru

Article

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Has Service

AAV1

Insert

fDIO-mNeonGreen

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

March 15, 2019

Availability

Academic Institutions and Nonprofits only

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mCherry-Tubulin-C-18

Plasmid

#55148

Purpose

Localization: Microtubules, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Article

Fiolka et al Proc Natl Acad Sci U S A. 2012 Apr 3;109

Insert

Tubulin (TUBA1B Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only
pC0040-LwaCas13a crRNA backbone

Plasmid

#103851

Purpose

Backbone for cloning guides that are compatible with LwaCas13a. Contains a 5' direct repeat. Clone using BbsI (BpiI). F overhang AAAC. R overhang AAAA

Depositor

Feng Zhang

Article

Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

Insert

U6-LwaCas13aDR-BbsI-BbsI-polyT

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-myc-hIFITM3

Plasmid

#58461

Purpose

Expresses human IFITM3 with an N-terminal myc tag in mammalian cells.

Depositor

Jacob Yount

Article

Chesarino et al J Biol Chem. 2014 Apr 25;289(17):11986-9

Insert

IFITM3 (IFITM3 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

CMV IE

Available since

Sept. 9, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-CCR5

Plasmid

#98943

Purpose

Mammalian expression plasmid for untagged human CCR5

Depositor

Erik Procko

Article

Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

Insert

CCR5 (CCR5 Human)

Use

Tags

Expression

Mammalian

Mutation

Full-length, wildtype

Promoter

CMV

Available since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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Lmx1b

Plasmid

#34997

Depositor

Malin Parmar

Article

Pfisterer et al Proc Natl Acad Sci U S A. 2011 Jun 21;10

Insert

LIM homeobox transcription factor 1-beta (Lmx1b Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 6, 2012

Availability

Academic Institutions and Nonprofits only

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pYFP-RPB1aAmr

Plasmid

#75284

Purpose

Expresses alpha-amanitin resistant EYFP tagged POLR2A in mammalian cells

Depositor

Robert Singer, Xavier Darzacq

Article

Darzacq et al Nat Struct Mol Biol. 2007 Sep;14(9):796-

Insert

POLR2A (POLR2A Human)

Use

Tags

EYFP

Expression

Mammalian

Mutation

N792D alpha-amanitin–resistant

Promoter

CMV

Available since

July 25, 2016

Availability

Academic Institutions and Nonprofits only

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APOBEC3G_pLX307

Plasmid

#98345

Purpose

Lentiviral expression of APOBEC3G

Depositor

William Hahn, Sefi Rosenbluh

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

APOBEC3G (APOBEC3G Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

E1Fa

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CMV-mCherry-hPMCA2w/b

Plasmid

#111570

Purpose

Expresses a plasma membrane calcium pump hPMCA2w/b

Depositor

Baljit Khakh

Article

Yu et al Neuron. 2018 Aug 27. pii: S0896-6273(18)

Insert

Plasma membrane calcium pump 2, variant w/b (ATP2B2 Human)

Use

Tags

mCherry

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-FLAG-Ku70

Plasmid

#46957

Purpose

Plasmid for cloning and epression of GFP-FLAG tagged human Ku70 (N-terminal tag). Confers resistance to G418.

Depositor

Steve Jackson

Article

Britton et al J Cell Biol. 2013 Jul 29.

Insert

Ku70 (XRCC6 Human)

Use

Tags

GFP-FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 20, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mhYFP

Plasmid

#186533

Purpose

Monomeric hyperfolder YFP fluorescent protein: cytosolic expression in mammalian cells

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

Monomeric hyperfolder YFP

Use

Tags

Expression

Mammalian

Mutation

hfYFP-S147P/L195M/V206K

Promoter

CMV

Available since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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CSII-PPM1H-mApple

Plasmid

#198473

Purpose

Mammalian expression of PPM1H-mApple

Depositor

Suzanne Pfeffer

Article

Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

Insert

PPM1H-mApple (PPM1H Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 18, 2023

Availability

Academic Institutions and Nonprofits only

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