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Plasmid#80263Purpose3rd generation lentiviral gRNA plasmid, non-targeting control gRNADepositorInsertNon-targeting control gRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceAug. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
His-Tev-UbE1
Plasmid#186804PurposeCodon-optimized bacterial expression plasmid. Expresses ubiquitin activating enzyme His-Tev-UbE1.DepositorInsertHis-Tev-UbE1 (UBA1 Human)
UseTagsHis6-TevExpressionBacterialMutationPromoterAvailable sinceMarch 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL0_9 [pUB110 ORI]
Plasmid#198924PurposeLevel 0 partDepositorInsertpUB110 ORI
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKRaION1-N1
Plasmid#177801PurposeMammalian expression of green fluorescent potassium indicator KRaION1DepositorInsertKRaION1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCTCON2 Aga2P-HRP
Plasmid#73152PurposeFull-length horseradish peroxidase (HRP) fused to the C-terminus of the yeast mating protein Aga2P. Galactose-inducible expression.DepositorInsertAga2P-HRP
UseTagsHA tag and myc tagExpressionYeastMutationPromoterGAL1Available sinceJune 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
TFORF0846
Plasmid#141732PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertYBX1 (YBX1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEZY19
Plasmid#18668PurposepET19b converted Gateway Destination vector for expressing His tagged fusion proteins in bacteria. See note in comments section below regarding the mutated enterokinase site.DepositorTypeEmpty backboneUseGateway destination vectorTagsHisExpressionBacterialMutationPromoterT7Available sinceJuly 26, 2008AvailabilityAcademic Institutions and Nonprofits only -
PLIN2-V5-APEX2
Plasmid#170573PurposeA C-terminal APEX2 fusion to the mouse perilipin-2 protein PLIN2DepositorInsertPLIN2 (Plin2 Mouse)
UseLentiviralTagsV5-APEX2ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2 TDP-43 5F-L YFP
Plasmid#84914PurposeMammalian expresion of TDP-43 5F-L YFPDepositorInsertTDP-43 (TARDBP Human)
UseTagsYFPExpressionMammalianMutationF147L, F149L, F194L, F229L, F231LPromoterCMVAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLV-mScarlet-I
Plasmid#159172PurposeTo express a bright monomeric red FP for labelling and detection of mammalian cellsDepositorInsertmScarlet-I
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationCodons were optimized to Human codon usagePromoterCMVAvailable sinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZ_P-AOX1-eGFP
Plasmid#126718Purposeyeast plasmid overexpressing eGFPDepositorInsertegfp
UseTagsExpressionYeastMutationPromoterAOX1Available sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
TRE-KRAB-dCas9-IRES-BFP
Plasmid#85449PurposeLentiviral vector expressing a KRAB-dCas9 fusion protein and BFPDepositorInsertKRAB-dCas9-IRES-BFP
UseCRISPR and LentiviralTags2x NLS, HA Tag, and KRAB domainExpressionMammalianMutationPromoterTRE3GAvailable sinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAG654 pFAST
Plasmid#172861PurposeExpresses pFAST in mammalian cellsDepositorInsertpFAST
UseTagsMyc tag (C terminal on insert)ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
AbVec_IGKC
Plasmid#183702PurposeEncode human Ig kappa chain constant region. For cloning variable region and mAb expression.DepositorInsertIGKC (IGKC )
UseTagsExpressionMammalianMutationPromoterSP6Available sinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
Flag-GFP11-pDEST-N
Plasmid#118366PurposeBimolecular Fluorescence Complimentation (BiFC) assay. Gateway compatible N-terminal vector with BiFC-tag (GFP11-Flag) to screen interaction partners.DepositorTypeEmpty backboneUseDestinationTagsGFP11-FlagExpressionMammalianMutationPromoterAvailable sinceNov. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3_OCT4
Plasmid#26816DepositorInsert5'UTR-OCT4-3'UTR (POU5F1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 17, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pgRNA-modified
Plasmid#84477Purposeguide RNA expression lentiviral constructDepositorInsertguide RNA
UseLentiviralTagsExpressionMammalianMutationPromotermU6Available sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLVX-IRES-Hygro_GCaMP6s
Plasmid#164591PurposeStable integration and expression of GCaMP6s in mammalian cellsDepositorInsertGCaMP6s
UseLentiviralTags6xHisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
mAzurite-8xbiRhoBAST-24xMS2v6
Plasmid#196352PurposeExpression of mAzurite mRNA with 8xbiRhoBAST-24xMS2v6 in 3'UTRDepositorInsertmAzurite-8xbiRhoBAST-24xMS2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mNaChBac-T2A-tdTomato
Plasmid#60650PurposeExpresses mNaChBac-T2A-tdTomatoDepositorInsertmNaChBac-T2A-tdTomato
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 24, 2014AvailabilityAcademic Institutions and Nonprofits only