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Plasmid#108748PurposeExpresses aa28 to C-terminus of Flp recombinase fused to GAI CIDDepositorInsertFlp recombinase fragment aa28 to C-terminus
UseCre/Lox and Synthetic BiologyTagsGAI and NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc-hIFITM3-Y20A
Plasmid#58462PurposeExpresses human IFITM3-Y20A with an N-terminal myc tag in mammalian cells.DepositorInsertIFITM3 (IFITM3 Human)
UseTagsmycExpressionMammalianMutationTyrosine 20 to alaninePromoterCMV IEAvailable sinceSept. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBABE puro EGFP
Plasmid#128041PurposeRetroviral vector with N terminal EGFPDepositorTypeEmpty backboneUseRetroviralTagsEGFPExpressionMammalianMutationPromoterAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
WT PBRM1- TRIPZ-neo
Plasmid#107406PurposeMammalian expression, inducible PBRM1DepositorInsertPBRM1 (PBRM1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
p416-TEF-membrane localization signal-tagBFP-wrmScarlet11-TEF terminator
Plasmid#158585PurposeExpresses membrane localized tagBFP with wrmScarlet11 C-terminal fusion in S. cerevisiaeDepositorInsertmembrane localization signal-tagBFP-wrmScarlet11
UseTagstagBFPExpressionYeastMutationGGGS linkerPromoterTEFAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC19_dTAG-KANSL3-targeting
Plasmid#170755PurposeDonor plasmid for BSD-P2A-2xHA-dTAG knock-in into the N-terminus of the mouse Kansl3 locusDepositorInsertBSD-P2A-2xHA-dTAG
UseMouse TargetingTagsBSD-P2A-2xHA-dTAGExpressionMutationPhenylalanine 36 to valine in FKBP12PromoterAvailable sinceJune 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-CMV-Puro-2A-NLS-FLAG-cGAS
Plasmid#127656PurposeOver-express NLS-FLAG-cGASDepositorInsertcGAS (CGAS Human)
UseLentiviralTagsFlagExpressionMutationPromoterAvailable sinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
Proinsulin-NanoLuc in pLX304
Plasmid#62057PurposeLuminescent reporter of insulin secretionDepositorInsertProinsulin-NanoLuc (Ins2 Mouse, Synthetic)
UseLentiviral and LuciferaseTagsExpressionMammalianMutationInserted NanoLuc into C-peptide of mouse Ins2 cod…PromoterCMVAvailable sinceJan. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
Human CRISPRi sgRNA library Dolcetto in backbone XPR_050, Set A
Pooled Library#92385PurposeCrispriDepositorHas ServiceLentiviral PrepUseLentiviralAvailable sinceJuly 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-EGFP-RAB7A-IRES-Puromycin
Plasmid#133027PurposeExpresses EGFP-tagged late endosome markerDepositorInsertRAB7A (RAB7A Human)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterHuman elongation factor 1 alphaAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC19_HDRT_TRAC_CD19-CAR
Plasmid#183473PurposeEncodes for a homology-directed DNA repair template for the insertion of a second generation CD19-specific CAR into the TRAC geneDepositorUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
MSCV-EGFP
Plasmid#91975PurposeEGFP reporter cell line control (no miR-19 sites)DepositorInsertEGFP
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
plenti_DCLK1
Plasmid#163625PurposeExpresses DCLK1DepositorInsertDCLK1 (DCLK1 Human)
UseLentiviralTagsV5ExpressionMutationPromotersv40Available sinceMarch 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
ABE8.20-m
Plasmid#136300Purposeexpresses ABE8.20-m in mammalian cellsDepositorInsertABE8.20-m
UseTagsC-terminal BPNLSExpressionMammalianMutationD10A in S. pyogenes Cas9, TadA mutations describe…PromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNQL004-SOX2-FKBPV-HA2-P2A-mCherry targeting construct
Plasmid#175552PurposeTargeting vector to introduce an FKBPV-HA2-P2A-mCherry cassette at the mouse SOX2 locus. Degradation Tag (dTAG) system.DepositorInsertSOX2 (Sox2 Mouse)
UseMouse TargetingTagsFKBPV-HA2-P2A-mCherryExpressionMammalianMutationnonePromoterAvailable sinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
DT480
Plasmid#80413PurposeExpresses 480 interrupted CUG repeats in the context of human DMPK genomic segment containing exons 11-15 with repeats inserted at site in exon 15 that contains the repeatsDepositorInserthuman DMPK exons 11-15 with 480 interrupted CTG repeats (DMPK Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSNAP-CLC-EYFP
Plasmid#38011DepositorInsertClathrin, light polypeptide (LCa) (Clta Mouse)
UseTagsEYFP and SNAP26mExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceSept. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
TFORF0372
Plasmid#141593PurposeLentiviral vector for overexpressing the NFYB transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertNFYB (NFYB Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
L444P GBA pcDNA3.1
Plasmid#188582PurposeEctopic expression L444P GBADepositorInsertGBA (GBA1 Human)
UseTagsExpressionMammalianMutationL444PPromoterCMVAvailable sinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
iF53 PB-Zim3-dCas9-mScarlet-Hygro
Plasmid#204726PurposeConstitutive CRISPRi by Zim3-dCas9InsertZim3-dCas9
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only