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FUGW-UBC-EGFP-2A-BoNT-B (LC)

Plasmid

#185722

Purpose

Lentiviral expression of GFP and botulinum neurotoxin type B light chain from UbC promoter

Depositor

Jun Ding

Article

Albarran et al Nat Neurosci. 2023 Jun;26(6):997-1007. d

Insert

botulinum neurotoxin type B light chain

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hUbC

Available since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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pMADM-beta

Plasmid

#36891

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Inserts

Beta-Geo
thymidine kinase

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG (chicken beta actin promoter and CMV enhancer…

Available since

Aug. 10, 2012

Availability

Academic Institutions and Nonprofits only

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pVITRO-HPV6 L1L2

Plasmid

#52589

Purpose

Expresses HPV6 L1 and L2 in mammalian cells

Depositor

Richard Roden

Article

Kwak et al PLoS One. 2014 May 9;9(5):e97232. doi: 1

Inserts

HPV6 L1
HPV6 L2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 21, 2014

Availability

Academic Institutions and Nonprofits only

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pFS478

Plasmid

#89066

Purpose

KanMX Z3EV promoter cassette

Depositor

Nick Rhind

Article

Ohira et al Yeast. 2017 Aug;34(8):323-334. doi: 10.1

Insert

Z3EV promoter cassette

Use

Yeast integration vector

Tags

Expression

Mutation

yeast Gal1 promoter modified with multiple Zif268…

Promoter

Z3EV promoter

Available since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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PAX6 donor plasmid

Plasmid

#105239

Purpose

Knock in H2B-GFP into the human PAX6 locus

Depositor

Lorenz Studer

Article

Tchieu et al Cell Stem Cell. 2017 Sep 7;21(3):399-410

Insert

PAX6 Homologous recombination ARMS with a P2A-H2B-GFP and Puromycin selection cassette

Use

TALEN

Tags

Expression

Mutation

Promoter

Available since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

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pJMK077: CaMKIIa QuasAr2-TS-GCaMP6f

Plasmid

#72304

Purpose

Combines a gCaMP-based calcium indicator and an Arch-based voltage indicator

Depositor

Adam Cohen

Article

Dempsey et al J Pharmacol Toxicol Methods. 2016 May 13

Insert

CaViar

Use

Lentiviral

Tags

TS

Expression

Mammalian

Mutation

Promoter

CaMKIIa

Available since

May 23, 2016

Availability

Academic Institutions and Nonprofits only

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pVITRO-HPV68 L1L2

Plasmid

#52587

Purpose

Expresses HPV68 L1 and L2 in mammalian cells

Depositor

Richard Roden

Article

Kwak et al PLoS One. 2014 May 9;9(5):e97232. doi: 1

Inserts

HPV68 L1
HPV68 L2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 20, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV-UGI-nCas9(H840A)-GSVG

Plasmid

#187411

Purpose

DddA GSVG variant

Depositor

Jin-Soo Kim

Article

Mok et al Nat Commun. 2022 Jul 12;13(1):4038. doi:

Insert

DddA GSVG variant

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pRCC-N

Plasmid

#81192

Purpose

Expression of Cas9 and gRNA cassette in S. cerevisiae; Nourseothricin Resistance

Depositor

Eckhard Boles

Article

Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

Inserts

Cas9
empty gRNA cassette

Use

CRISPR

Tags

NTS

Expression

Yeast

Mutation

Please see depositor comments below. and WT - cod…

Promoter

ROX3 and SNP52p

Available since

Sept. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pT2ADW-4146NES(ROCK)

Plasmid

#162485

Purpose

For generating transgenic mice expressing ROCK biosensor based on FRET.

Depositor

Michiyuki Matsuda

Article

Imanishi et al Am J Pathol. 2020 Oct 15. pii: S0002-944

Insert

ROCK biosensor based on FRET.

Use

Tags

YPet and ECFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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JD690

Plasmid

#160397

Purpose

Binary vector pGWB14 including citrus GRF4-GIF1 under 35S promoter/ Plant transformation

Depositor

Jorge Dubcovsky

Article

Debernardi et al Nat Biotechnol. 2020 Oct 12. pii: 10.103

Insert

35S::citrus GRF4-GIF1 chimera

Use

Tags

Expression

Mutation

Promoter

35S

Available since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pPRIME-dsRed-shscramble

Plasmid

#71384

Purpose

Expresses dsRed and scramble shRNA under the control of the CMV promoter in eukaryotic cells

Depositor

William Wisden

Article

Yu et al Neuron. 2015 Jun 17. pii: S0896-6273(15)

Insert

CMV promoter-dsRed-mir30-shscramble

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

CMV enhancer/promoter

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pR26 GFP Dest

Plasmid

#74283

Purpose

Gene targeting vector for the mouse Rosa26 locus, including a loxP flanked stop cassette and GFP, for gateway cloning

Depositor

Ralf Kuehn

Article

Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

Insert

Rosa26 5-homology region

Use

Tags

Expression

Mutation

Promoter

Available since

April 27, 2016

Availability

Academic Institutions and Nonprofits only

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psiCHECK2-2A-3xFLAG-SBP-2A

Plasmid

#159584

Purpose

Luciferase reporter for ribosome pausing control

Depositor

Shintaro Iwasaki

Article

Han et al Cell Rep. 2020 May 5;31(5):107610. doi:

Inserts

2A-3xFLAG-SBP-2A
EIF2S3 5UTR

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pUDP293

Plasmid

#204228

Purpose

Episomal yeast plasmid expressing the Ercas12a and ErCas12a gRNA

Depositor

Jean-Marc Daran

Article

Bennis et al FEMS Yeast Res. 2023 Jan 4;23:foad043. d

Inserts

Eubacterium rectale cas12a (Ercas12a)
BsaI_GFP drop-out_BsaI

Use

Synthetic Biology

Tags

NLS

Expression

Mutation

codon optimized for S. cerevisiae

Promoter

Saccharomyces cerevisiae PGK1 and Saccharomyces c…

Available since

Sept. 22, 2023

Availability

Academic Institutions and Nonprofits only

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rAAV-syn::FLEX-rev::PSAML141F,Y115F:GlyR-IRES-GFP

Plasmid

#32481

Depositor

Scott Sternson

Article

Magnus et al Science. 2011 Sep 2;333(6047):1292-6.

Insert

PSAML141F,Y115F-GlyR

Use

AAV and Cre/Lox; Adeno-associated virus, flex swi…

Tags

IRES-EGFP

Expression

Mutation

Promoter

Synapsin

Available since

Jan. 13, 2012

Availability

Academic Institutions and Nonprofits only

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pDae069 (eMutaT7[PmCDA1] with optimized RBS)

Plasmid

#187621

Purpose

Expresses eMutaT7[PmCDA1] and UGI for mutagenizing genes downstream from a T7 promoter in e.coli

Depositor

Seokhee Kim

Article

Seo et al Nucleic Acids Res. 2023 Apr 18:gkad266.

Insert

PmCDA1-T7RNAP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pDD268

Plasmid

#132523

Purpose

mNG^SEC^3xFlag vector with ccdB markers for cloning homology arms

Depositor

Daniel Dickinson, Bob Goldstein

Article

Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

Insert

mNG-C1^SEC^3xFlag

Use

CRISPR and Cre/Lox

Tags

3xFlag and C. elegans codon-optimized mNG

Expression

Worm

Mutation

Promoter

Available since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-smURFP-T2A-mCardinal

Plasmid

#80348

Purpose

Lentiviral transfer vector under the CMV promoter that expresses smURFP and mCardinal.

Depositor

Erik Rodriguez, Roger Tsien

Article

Rodriguez et al Nat Methods. 2016 Aug 1. doi: 10.1038/nm

Inserts

smURFP
mCardinal

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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Plasmid L5 attB::Pleft* mScarlet

Plasmid

#169410

Purpose

Chromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for mScarlet

Depositor

Clifton Barry III

Article

Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

Inserts

pLeft
mScarlet proper

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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