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Showing: 11661 - 11680 of 24661 results

  4. Cas9-NAT

    Plasmid
    #64329
    Purpose
    Cas9 expression cassette carried by a yeast single-copy episome plasmid
    Depositor
    Yong-Su Jin
    Article
    Zhang et al Appl Environ Microbiol. 2014 Dec;80(24):
    Insert
    Human-optimized S. pyogenes Cas9 with Clonnat marker gene expression cassette
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    64329-plasmid-map-sequence-id-227458

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  7. pMax_ScaI_BFP

    Plasmid
    #177824
    Purpose
    Site-specific mutagenesis of tagBFP for the purposes of creating a clean double strand break for the purposes of measuring NHEJ activity via Host Cell Reactivation (HCR).
    Depositor
    Leona Samson, Zachary Nagel
    Article
    Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111
    Insert
    tagBFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    5' inserted ScaI RE cut site
    Promoter
    CMV
    Available since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    177824-plasmid-map-sequence-id-348950

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  12. pEVOL-ABK

    Plasmid
    #126035
    Purpose
    Efficient expression of tRNA synthetase/tRNA for the incorporation of a photo-cross-linker, 3’-azibutyl-N-carbamoyl-lysine (AbK) into recombinant protein by amber codon (TAG) suppression in E. coli.
    Depositor
    Andrea Musacchio
    Article
    Pan et al Nat Commun. 2019 Sep 6;10(1):4046. doi:
    Inserts
    tRNA synthetase 1
    tRNA synthetase 2
    tRNA for TAG codon
    Use
    Tags
    Expression
    Bacterial
    Mutation
    L274M, C313A, Y349F
    Promoter
    araBAD promoter, glnS promoter, and proK promoter
    Available since
    Sept. 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    126035-plasmid-map-sequence-id-244699

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  14. pCR2.1_HBB_N

    Plasmid
    #87847
    Purpose
    pCR2.1-based plasmid holding a 309-bp fragment containing the exon-1-exon-2 splice border of normal human β-globin mRNA
    Depositor
    Marina Kleanthous
    Article
    Patsali et al Int J Mol Sci. 2020 Sep 11;21(18). pii:
    Insert
    Homo sapiens hemoglobin subunit beta (HBB), Normal cDNA fragment (Exon1/2) (HBB Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    87847-plasmid-map-sequence-id-313714

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  17. pMax_mPlum_C207G/T208C

    Plasmid
    #177831
    Purpose
    Site-specific mutagenesis of mPlum for the purposes of creating a site-specific O6-MeG lesion for the purposes of measuring MGMT activity via Host Cell Reactivation (HCR).
    Depositor
    Leona Samson, Zachary Nagel
    Article
    Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111
    Insert
    mPlum_C207G/T208C
    Use
    Tags
    Expression
    Mammalian
    Mutation
    C207G + T208C
    Promoter
    CMV
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177831-plasmid-map-sequence-id-348956

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Showing: 11661 - 11680 of 24661 results