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Plasmid#52670PurposeOverexpression of GFP-tagged α-actinin-3DepositorInsertACTN3 (ACTN3 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
MSP2133
Plasmid#72249PurposeHuman expression plasmid for SpCas9-HF4 variant: CMV-T7-humanSpCas9-HF4(Y450A, N497A, R661A, Q695A, Q926A)-NLS-3xFLAGDepositorInsertmammalian codon-optimized Streptococcus pyogenes Cas9 HF4(Y450A/N497A/R661A/Q695A/Q926A)-NLS-3xFlag
UseCRISPRTags3x FLAG and NLSExpressionMammalianMutationY450A, N497A, R661A, Q695A, and Q926APromoterCMVAvailable sinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCT-CMV-UBE3B-copGFP-Puro
Plasmid#107342PurposeExpresses UBE3B fused to copGFP in mammalian cells.DepositorInsertUbiquitin-protein ligase E3B (UBE3B Human)
UseLentiviralTagscopGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 19, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
FUW-TetO-Ctcf
Plasmid#40801DepositorInsertCtcf (Ctcf Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterMinimal CMV + TetOAvailable sinceOct. 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTol2-10xUAS-Voltron-ST
Plasmid#119040PurposeUAS-driven expresion of soma-localized Voltron in zebrafishDepositorInsertVoltron-ST
UseZebrafish expressionTagsExpressionMutationPromoterE1bAvailable sinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMOD_C2200
Plasmid#91082PurposeModule C, Promoter: none – to be combined with gRNA array in module B, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers (only works with pMOD_B2203), Terminator: 35SDepositorInsertSapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers
UseCRISPRTagsExpressionMutationPromoternone (will be fused to gRNA array in MODULE B)Available sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAX APP AENATA
Plasmid#30144DepositorInsertAmyloid Precursor Protein (APP Human)
UseTagsExpressionMammalianMutationYENPTY-->AENATAPromoterAvailable sinceJuly 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCT-CMV-UBE3B(C1036A)-copGFP-Puro
Plasmid#107344PurposeExpresses UBE3B mutant C1036A fused to copGFP in mammalian cells.DepositorInsertUBE3B(C1036A)copGFP (UBE3B Human)
UseLentiviralTagscopGFPExpressionMammalianMutationmutation C1036APromoterCMVAvailable sinceApril 19, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTol2-10xUAS-Voltron
Plasmid#119039PurposeUAS-driven expresion of Voltron in zebrafishDepositorInsertVoltron
UseZebrafish expressionTagsExpressionMutationPromoterE1bAvailable sinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
hMLKL NB(1-140)-2xFv-Venus
Plasmid#106076Purposeexpression in mammalian cellsDepositorInserthuman MLKL NB(1-140)-2xFv-Venus (MLKL Human)
UseRetroviral; Tet-on retrovirusTagsVenusExpressionMammalianMutationamino acids 1-140PromoterDox-inducibleAvailable sinceMarch 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV_Efs_hSpCas9_NLS_FLAG-SV40
Plasmid#97307PurposeAAV vector for encoding a human codon-optimized SpCas9 driven by EFs promoterDepositorInserthumanized S. pyogenes Cas9
UseAAV and Mouse TargetingTagsFlagExpressionMammalianMutationPromoterEFSAvailable sinceAug. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO-H2B-GFP-2A-oG-WPRE-hGH
Plasmid#74289PurposepAAV vector to express H2B-GFP and oG (optimized Glycoprotein) in a Cre-dependent mannerDepositorInsertH2B-GFP and oG (optimized Glycoprotein)
UseAAVTagsExpressionMammalianMutationchimeric glycoproteinPromoterEf1aAvailable sinceApril 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAX APP delta CT
Plasmid#30143DepositorInsertAmyloid Precursor Protein (APP Human)
UseTagsExpressionMammalianMutationdeletion of C-terminal intracellular domain (afte…PromoterAvailable sinceJuly 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
MSP2135
Plasmid#72248PurposeHuman expression plasmid for SpCas9-HF2 variant: CMV-T7-humanSpCas9-HF2(N497A, R661A, Q695A, Q926A, D1135E)-NLS-3xFLAGDepositorInsertmammalian codon-optimized Streptococcus pyogenes Cas9 HF2(N497A/R661A/Q695A/Q926A/D1135E)-NLS-3xFlag
UseCRISPRTags3x FLAG and NLSExpressionMammalianMutationN497A, R661A, Q695A, Q926A, and D1135EPromoterCMVAvailable sinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAX APPs-695-alpha
Plasmid#30147DepositorInsertAmyloid Precursor Protein - soluble (APP Human)
UseTagsExpressionMammalianMutationamino acids 1–612PromoterAvailable sinceAug. 15, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMOD_C2518
Plasmid#91087PurposeModule C, Promoter: TaU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol IIIDepositorInsertEsp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)
UseCRISPRTagsExpressionMutationPromoterTaU6Available sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCCLc-MND-B2705-KK10-SABR
Plasmid#119053Purpose3rd gen transfer vector. HLA-B2705 linked to b2-microglobulin and CD28-CD3z signaling domain. Contains the HIV(gag) KK10 epitope "KRWIILGLNK" cloned via BsmBIDepositorInsertB2705-KK10-SABR
UseLentiviralTagsExpressionMammalianMutationPromoterMNDAvailable sinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMOD_C2515
Plasmid#91083PurposeModule C, Promoter: AtU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol IIIDepositorInsertEsp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)
UseCRISPRTagsExpressionMutationPromoterAtU6Available sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
XE248 pCDNA3.1+zeo-VSV-KLHL12
Plasmid#16761DepositorInsertpCDNA3.1+zeo-VSV-KLHL12 (KLHL12 Human)
UseTagsVSVExpressionMammalianMutationPromoterAvailable sinceMarch 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
hMLKL NB(1-182)-2xFv-flag
Plasmid#106075Purposeexpression in mammalian cellsDepositorInserthuman MLKL NB(1-182)-2xFv-flag (MLKL Human)
UseRetroviral; Tet-on retrovirusTagsflagExpressionMammalianMutationamino acids 1-182PromoterDox-inducibleAvailable sinceMarch 1, 2018AvailabilityAcademic Institutions and Nonprofits only