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Plasmid#218678Purposeexpressing SARS-CoV-2 JN.1 spike protein for pseudovirus productionDepositorInsertSpike (JN.1)
UseTagsExpressionMammalianMutationL455S on the basis of BA.2.86PromoterCMVAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR-mCherry
Plasmid#75161PurposeDerived from LentiCRISPR v1 but expresses mCherry instead of puromycin resistance. 3rd generation.DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsmCherryExpressionMammalianMutationPromoterAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
ER-mGreenLantern
Plasmid#164463PurposeLocalization and retention of mGreenLantern fluorescent protein in the ERDepositorInsertmGreenLantern
UseTagsCalreticulin and KDELExpressionMammalianMutationClover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…PromoterCMVAvailable sinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTol2_LSEC:Cas9green; erbb2_gRNA171
Plasmid#199338PurposepDEL135; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous erbb2 sgRNADepositorInsertsLSEC
Cas9
erbb2 sgRNA
UseTol2 destination vectorTagsExpressionMutationPromoterAvailable sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-hChR2(H134R)-mCherry
Plasmid#26976PurposeAAV expression of humanized ChR2 with H134R mutation fused to mCherry, for optogenetic excitationDepositorHas ServiceAAV5, AAV8, and AAV9InserthChR2(H134R)
UseAAVTagsmCherryExpressionMammalianMutationH134RPromoterhSynAvailable sinceFeb. 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
pTrcHisB-TRF2
Plasmid#50488PurposeE. Coli N-term HexHis Tagged Protein Expression VectorDepositorInsertTRF2 (TERF2 Human)
UseTags6x His, T7 tag, and TEV protease siteExpressionBacterialMutationContains amino acids 3-500PromoterpTrcAvailable sinceFeb. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
openCIDAR MoClo Toolkit
Plasmid Kit#1000000227PurposeThis collection includes broad host range, pBBR1-based vectors compatible with the CIDAR MoClo cloning standard. These vectors replicate in many bacteria across Pseudomonadota.DepositorAvailable sinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
psfGFP-LAMP1-mCherry (pHLARE)
Plasmid#164477PurposeLysosomal pH biosensor consisting of sfGFP-rat Lamp1-mCherry.DepositorInsertLAMP1 (Lamp1 Rat)
UseTagsmCherry and superfolder GFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pC0079 RESCUE-S
Plasmid#130662PurposepCMV-dCas13b6-mapkNES-GS-dADAR2(E488Q/V351G/S486A/T375A/S370C/P462A/N597I/L332I/I398V/K350I/M383L/D619G/S582T/V440I/S495N/K418E/S661T) V16SDepositorInsertdRanCas13b-ADAR2dd
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pAAV.Syn.NES.jRCaMP1a.WPRE.SV40
Plasmid#100848PurposeAAV expression of jRCaMP1a, a red fluorescent calcium sensor protein, from the Synapsin promoterDepositorHas ServiceAAV1 and AAV9InsertjRCaMP1a
UseAAVTagsExpressionMammalianMutationPromoterSynapsinAvailable sinceNov. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLR5-CAG-hMCP-mScarlet-I-NLS
Plasmid#174878PurposePiggyBac transposon vector for expression of MCP-mScarlet-I-NLSDepositorInsertMCP-mScarlet-I-NLS
UsePiggybac vectorTagsExpressionMammalianMutationPromoterAvailable sinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
HA-Elongin B-pcDNA3.1(+)-Zeo
Plasmid#20000DepositorInserttranscription elongation factor B (ELOB Human)
UseTagsHA-tagExpressionMammalianMutationwild typePromoterAvailable sinceApril 6, 2009AvailabilityAcademic Institutions and Nonprofits only -
Lenti-117G-hyA3A-BE4max
Plasmid#157945PurposeLentiviral expression of hyA3A-BE4maxDepositorInsertLenti-117G- hyA3A-BE4max
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
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TFORF2165
Plasmid#141953PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertGRHL3 (GRHL3 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBSFI-myr-akt1
Plasmid#49186PurposecDNA of Akt1 with myristyolation signal in cloning vectorDepositorInsertAkt1 (Akt1 Mouse)
UseAdaptor plasmidTagsHA tag and myristolation signalExpressionMutationPromoterT7Available sinceDec. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-ROBO2-Fc(DAPA)-AviTag-6xHis
Plasmid#156991PurposeMammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertROBO2 (ROBO2 Human)
UseTagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
px459-TDP-43 sgRNA1
Plasmid#133762PurposeExpresses Cas9 and human TDP-43 sgRNADepositorInsertTDP-43 (TARDBP Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceDec. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
TFORF2528
Plasmid#144127PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertERCC6 (ERCC6 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 13, 2023AvailabilityAcademic Institutions and Nonprofits only