Addgene

We narrowed to 28,098 results for: eng

Showing: 11581 - 11600 of 28098 results

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pOVC1_X_MxCarH-CBD

Plasmid

#131189

Purpose

Expresses MxCarH-CBD (Myxococcus xanthus) preceded by restriction sites

Depositor

Harald Janovjak

Article

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Insert

MxCarH-CBD

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pOVC1_X_TtCarH-CBD

Plasmid

#131190

Purpose

Expresses TtCarH-CBD (Thermus termophilus) preceded by restriction sites

Depositor

Harald Janovjak

Article

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Insert

TtCarH-CBD

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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gRNA_tdtomato_reporter_1_ST1_2x_binding

Plasmid

#79368

Purpose

Assays activity of transcriptional activators fused to ST1 Cas9

Depositor

George Church

Article

Kiani et al Nat Methods. 2015 Sep 7. doi: 10.1038/nm

Insert

tdtomato

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

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BSP188

Plasmid

#110917

Purpose

For cloning by HR in yeast, Universal MosSCI compatible, has unc54 terminator cloned

Depositor

Alexander Mendenhall

Article

Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

Insert

unc54 Terminator

Use

Synthetic Biology; Yeast homologous recombination…

Tags

Expression

Worm

Mutation

Promoter

Available since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pCEP4-H031gp160

Plasmid

#123272

Purpose

Mammalian expression plasmid for Env from the H031 HIV-1 isolate

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (H031) Env

Use

Tags

CD5 leader peptide

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Available since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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VV228: sdChR(C138S E154A)-TS in fck

Plasmid

#58523

Purpose

Expresses sdChR(C138S E154A) in mammalian cells

Depositor

Adam Cohen

Article

Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

Insert

sdChR(C138S, E154A)-TS

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

changed Cysteine 138 to Serine; changed Glutamate…

Promoter

a-CamKII

Available since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pBDKRB2_TCS(Q’G)_NLS-HA-dCas9(C)_T2A_DHFR-PCP-VP64

Plasmid

#101110

Purpose

Encodes dCas9(C) with DHFR-PP7-VP64 fused to BDKRB2

Depositor

Tudor Fulga

Article

Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

Insert

BDKRB2-dCas9(C)_DHFR-PCP-VP64

Use

CRISPR and Synthetic Biology

Tags

Flag and HA

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pET20b-CeNLuc-6xHis

Plasmid

#135933

Purpose

Expresses the CeNLuc LumiFluor in bacterial cells.

Depositor

Antonio Amelio

Article

Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

Insert

CeNLuc LumiFluor

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 TCF3

Plasmid

#67110

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

TCF3 (TCF3 Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

Truncated isoform

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pMOD_A3601

Plasmid

#91051

Purpose

Module A, Promoter: 35S, Gene: dAtCas9-VP64, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

dAtCas9-VP64

Use

CRISPR

Tags

Expression

Mutation

Promoter

35S

Available since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pMT Draper-ITAM only-GFP

Plasmid

#126656

Purpose

Inducible expression of Draper-ITAM only-GFP mutant in S2 cells

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Draper with Cytoplasmic ITAM residues Tyr all others mutated to Phe

Use

Tags

Expression

Insect

Mutation

Cytoplasmic ITAM residues Tyr all others mutated …

Promoter

Available since

June 18, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-ZNF668

Plasmid

#42627

Depositor

Shiaw-Yih Lin

Article

Hu et al Cancer Res. 2011 Oct 15;71(20):6524-34.

Insert

ZNF668 (ZNF668 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

April 23, 2013

Availability

Academic Institutions and Nonprofits only
pAP1680

Plasmid

#137088

Purpose

Plasmid for PCR-generated donor DNA for rapid tagging of proteins with tagRFP

Depositor

Geraldine Seydoux

Article

Paix et al Curr Protoc Mol Biol. 2019 Dec;129(1):e1

Insert

tagRFP

Use

Pcr template for donor dna

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 13, 2020

Availability

Academic Institutions and Nonprofits only

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pBW214J115-Amp32C

Plasmid

#78649

Purpose

pSB3K3 carrying J115-30hrpR-32hrpS-t-hrpL-30gfp-t

Depositor

Baojun Wang

Article

Wang et al Nucleic Acids Res. 2014 Aug;42(14):9484-

Insert

J115-30hrpR-32hrpS-t-hrpL-30gfp-t

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

see insert

Available since

July 27, 2016

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 YWHAB

Plasmid

#67063

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

YWHAB (YWHAB Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
Park2_L (TAL 3012)

Plasmid

#36004

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

TAL array targeting Park2 (prkn Zebrafish)

Use

T7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 8, 2012

Availability

Academic Institutions and Nonprofits only

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Park2_R (TAL 3013)

Plasmid

#36005

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

TAL array targeting Park2 (prkn Zebrafish)

Use

T7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

Available since

April 27, 2012

Availability

Academic Institutions and Nonprofits only

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pconst #2-RFP

Plasmid

#81076

Purpose

constitutive promoter expressing RFP, biobrick construction

Depositor

Paul Adams, Jay Keasling

Article

Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

Insert

RFP

Use

P15a origin, constitutive promoter, kanr

Tags

Expression

Mutation

Promoter

Constitutive Promoter- BBa_J23104

Available since

Aug. 18, 2016

Availability

Academic Institutions and Nonprofits only

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ACS_19

Plasmid

#111651

Purpose

Funclib design ACS_19

Depositor

Sarel Fleishman

Article

Khersonsky et al Mol Cell. 2018 Oct 4;72(1):178-186.e5. d

Insert

ACS_19

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

pTac

Available since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-dSa-VPR mini.-1X snRP1

Plasmid

#99687

Purpose

Expresses dSa Cas9 fused to optimized combination of truncated activation domains with 17 nt snRP1 poly adenylation signal

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Use

AAV

Tags

VPR mini

Expression

Mutation

dead Cas9

Promoter

Available since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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