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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 3,312 results for: golden gate

Showing: 1141 - 1160 of 3312 results

  1. pAIP4_3HNX

    Plasmid
    #234199
    Purpose
    Heterologous protein expression of cyanovirin-N homolog (CV-N homolog) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    3HNX
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234199-plasmid-map-sequence-id-464164

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  2. pAIP4_4A02

    Plasmid
    #234194
    Purpose
    Heterologous protein expression of lytic chitin monooxygenase in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    4A02
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234194-plasmid-map-sequence-id-464159

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  3. pAIP4_5MDT

    Plasmid
    #234186
    Purpose
    Heterologous protein expression of rpb7-binding protein seb1 in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    5MDT (seb1 Fission Yeast)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234186-plasmid-map-sequence-id-464151

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  4. pAIP4_2GAS

    Plasmid
    #234145
    Purpose
    Heterologous protein expression of isoflavone reductase (IFR) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    2GAS
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234145-plasmid-map-sequence-id-464111

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  5. L1P137 - pAHP6-PhiC31-NLS-tUBQ1

    Plasmid
    #219702
    Purpose
    Level 1 pAHP6-PhiC31-NLS-tUBQ1, for xylem pole specific expression of PhiC31 integrase
    Depositor
    Jennifer Nemhauser
    Insert
    pAHP6:PhiC31-NLS:tUBQ1
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    June 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    219702-plasmid-map-sequence-id-435732

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  12. pBD027

    Plasmid
    #201310
    Purpose
    avrSr27-3 as Golden Gate compatible MoClo CDS part (no recognition by Sr27)
    Depositor
    Benjamin Schwessinger
    Insert
    avrSr27-3
    Use
    Tags
    Expression
    Plant
    Mutation
    removal of BsaI and BpiI sites
    Promoter
    Available since
    Oct. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    201310-plasmid-map-sequence-id-409519

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  13. U24m

    Plasmid
    #121026
    Purpose
    MoClo golden gate assembly BC part for RiboJ-BCD2 (low expression bi-cistronic RBS, engineered for context-independence, prefixed by self-cleaving RiboJ; see https://doi.org/10.1038/nmeth.2404).
    Depositor
    Richard Murray
    Insert
    RiboJ + BCD2
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121026-plasmid-map-sequence-id-238071

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  14. T14m

    Plasmid
    #121033
    Purpose
    MoClo golden gate assembly DE part for L3S1P13 (178x-strength synthetic terminator; see DOI: 10.1038/nmeth.2515). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    L3S1P13 (synthetic Voigt terminator)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121033-plasmid-map-sequence-id-236356

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  15. C90m

    Plasmid
    #120998
    Purpose
    MoClo golden gate assembly CD part for NahR-sfGFP fusion (Adam Meyer improved NahR (C75m) fused to superfolder GFP (C3m)). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    NahRsfGFP fusion
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120998-plasmid-map-sequence-id-236128

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  16. C92m

    Plasmid
    #121000
    Purpose
    MoClo golden gate assembly CD part for sfCFP-pdt3 (superfolder CFP developed by Ye Chen in Matthew Bennet's lab, with a pdt3 tag for mfLon protease).
    Depositor
    Richard Murray
    Insert
    sfCFPpdt3 (Chen,Bennett)
    Use
    Synthetic Biology
    Tags
    pdt3 mfLon degradation tag
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121000-plasmid-map-sequence-id-238058

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  17. C99m

    Plasmid
    #121003
    Purpose
    MoClo golden gate assembly CD part for codon-optimized mScarlet-I (fast-folding mScarlet variant, codon-optimized for E. coli; see https://www.fpbase.org/protein/mscarlet-i/).
    Depositor
    Richard Murray
    Insert
    mScarlet-I codon optimized
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121003-plasmid-map-sequence-id-236141

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  18. C100m

    Plasmid
    #121004
    Purpose
    MoClo golden gate assembly CD part sfYFP-ptd3 (superfolder YFP with pdt3 degradation tag for mfLon). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    sfYFP with pdt3 tag
    Use
    Synthetic Biology
    Tags
    pdt3 mfLon degradation tag
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121004-plasmid-map-sequence-id-236143

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  19. C101m

    Plasmid
    #121005
    Purpose
    MoClo golden gate assembly CD part sfCFP-LAA (superfolder CFP developed by Ye Chen in Matthew Bennet's lab, with LAA degradation tag for ssrA).
    Depositor
    Richard Murray
    Insert
    sfCFP with LAA tag
    Use
    Synthetic Biology
    Tags
    LAA degradation tag for ssrA
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121005-plasmid-map-sequence-id-236158

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  20. C102m

    Plasmid
    #121006
    Purpose
    MoClo golden gate assembly CD part treRT-LAA (synthetic Lac-family repressor chimera with LAA degradation tag for ssrA; see DOI: 10.1021/sb500262f).
    Depositor
    Richard Murray
    Insert
    treRT with LAA tag
    Use
    Synthetic Biology
    Tags
    LAA degradation tag for ssrA
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121006-plasmid-map-sequence-id-236159

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Showing: 1141 - 1160 of 3312 results