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We narrowed to 5,665 results for: golden gate

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Showing: 1141 - 1160 of 5665 results

  3. C114m

    Plasmid
    #121013
    Purpose
    MoClo golden gate assembly CD part for Cas9 (S. pyogenes gRNA-targeted endonuclease Cas9, with bbsI cut sites removed synonymously). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    dCas9 with no bbsI or BsaI sites
    Use
    Synthetic Biology
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121013-plasmid-map-sequence-id-245424

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  5. pR2B5

    Plasmid
    #245486
    Purpose
    pL1-R2-pAtUBI-RUBY-tRBCS (Golden Gate Level 1 module with a transcription unit expressing RUBY from the AtUBI promoter with the tRBC terminator for R2 position in Level 2 vector)
    Depositor
    Beth Rowan
    Insert
    pAtUBI-RUBY-tRBCS
    Expression
    Plant
    Promoter
    Polyubiquitin 10 gene from Arabidopsis thaliana
    Available since
    Nov. 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    245486-plasmid-map-sequence-id-492313

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  6. PB_gRNA_S.pyogenes_scaffold_BB

    Plasmid
    #226429
    Purpose
    PiggyBac transposon vector backbone for cloning of sgRNA compatible with S.pyogenes dCas9 enzyme. BbsI Golden Gate site upstream of the scaffold for protospacer insertion. Puromycin selection marker.
    Depositor
    Alon Goren
    Type
    Empty backbone
    Use
    CRISPR; Piggybac transposon
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Sept. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    226429-plasmid-map-sequence-id-458099

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  7. pUAP1

    Plasmid
    #63674
    Purpose
    Accepts DNA sequences via BpiI cloning sites, resulting in Level 0 standard parts for Golden Gate cloning. Parts can be released with a user-defined 4-base-pair, 5 prime overhangs using BsaI.
    Depositor
    Nicola Patron
    Publication
    Patron et al New Phytol. 2015 Jul 14. doi: 10.1111/np
    Insert
    RFP cloning selection cassette
    Use
    Synthetic Biology
    Available since
    April 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    63674-plasmid-map-sequence-id-106088

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  10. pYL001

    Plasmid
    #249611
    Purpose
    Cloning vector for PaqCI Golden Gate assembly of ISYmu1 gRNAs into TRV2 with mutation SlFT element
    Depositor
    Steven Jacobsen
    Publication
    Liu et al bioRxiv 2025.11.08.687402
    Type
    Empty backbone
    Expression
    Plant
    Available since
    Jan. 12, 2026
    Availability
    Academic Institutions and Nonprofits only
    249611-plasmid-map-sequence-id-496977

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  12. MoChlo: Modular Cloning Chloroplast Toolbox

    Plasmid kit
    #1000000156
    Purpose
    Library of standardized chloroplast-specific genetic modules for Golden-Gate cloning of synthetic operons into chloroplast transformation vectors.
    Depositor
    Scott Lenaghan
    Application
    Cloning and Synthetic Biology
    Vector type
    Plant Expression
    Cloning type
    Golden Gate (MoClo)
    Available since
    Aug. 15, 2019
    Availability
    Academic Institutions and Nonprofits only

  13. pR2B6

    Plasmid
    #245487
    Purpose
    pL1-R2-pLsUBI-RUBY-tLsUBI (Golden Gate Level 1 module with a transcription unit expressing RUBY from the LsUBI promoter with the tLsUBI terminator for R2 position in Level 2 vector)
    Depositor
    Beth Rowan
    Insert
    pLsUBI-RUBY-tLSUBI
    Expression
    Plant
    Promoter
    Polyubiquitin 4 gene (LOC111919935) from lettuce
    Available since
    Nov. 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    245487-plasmid-map-sequence-id-492314

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  16. IBMc233

    Plasmid
    #161939
    Purpose
    Carries Golden Gate substitution insert for IBM with M86 intein. PPhlF as C-lobe promoter
    Depositor
    Baojun Wang
    Publication
    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:
    Insert
    pSB1A3-BbsI-M86N-T-cat-PPhlF-RiboJ-B32-M86C-SapI
    Expression
    Bacterial
    Promoter
    PPhlF
    Available since
    March 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    161939-plasmid-map-sequence-id-327549

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Showing: 1141 - 1160 of 5665 results