We narrowed to 5,195 results for: golden gate
-
Plasmid Kit#1000000234PurposeGolden Gate genetic toolkit developed to help researchers identify functional plasmids and establish genetic tractability in non-model bacteria.DepositorApplicationCloning and Synthetic BiologyVector TypeBacterial ExpressionCloning TypeGolden Gate (Loop)Available SinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only
-
PB-hEF1A-MG-U6-sgRNA
Plasmid#175505PurposeAll-in-one piggyBac transposon destination vector for hEF1a expression of Mega Gate cloned elements and hU6 expression of Golden Gate cloned guide RNAsDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionMammalianPromoterhEF1a, U6Available SinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYL001
Plasmid#249611PurposeCloning vector for PaqCI Golden Gate assembly of ISYmu1 gRNAs into TRV2 with mutation SlFT elementDepositorTypeEmpty backboneExpressionPlantAvailable SinceJan. 12, 2026AvailabilityAcademic Institutions and Nonprofits only -
pBP_sgRNA
Plasmid#190128PurposeLevel 0 MoClo plasmid for Golden Gate cloning of sgRNAsDepositorTypeEmpty backboneUseCRISPRAvailable SinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pICSL4723-P1
Plasmid#191786PurposeLevel P Golden gate accepterDepositorTypeEmpty backboneExpressionPlantAvailable SinceJan. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCR8/GW:U6
Plasmid#85981PurposeGolden Gate Level 0 cassette encoding the U6 promoterDepositorInsertT. pseudonana U6 promoter
UseCRISPR; Diatom (t. pseudonana)PromoternoneAvailable SinceJan. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAGM4723:TpCC_Urease
Plasmid#85982PurposeGolden Gate Level 2 construct encoding Cas9-YFP and 2 urease-targeting gRNAsDepositorInsertsCas9
Urease-targeting gRNA 1
Urease-targeting gRNA 2
UseCRISPR; Diatom (t. pseudonana)TagsNLS and YFPPromoterFCP and U6Available SinceJan. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
EC10161
Plasmid#154060PurposeLevel 0.5 Golden Gate backbone, containing loxP sites.DepositorTypeEmpty backboneUseCre/Lox and Synthetic BiologyExpressionBacterialPromoterN/AAvailable SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pICSL90016
Plasmid#117514PurposeCas9_2 Golden Gate Level 0DepositorInsertBsaI:AATG:Cas9_2:GCTT:BsaI
UseCRISPRTagsNLS_SV40ExpressionPlantAvailable SinceNov. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYPQ135A
Plasmid#69277PurposeGolden Gate entry vector; 5th gRNA under AtU6 promoterDepositorTypeEmpty backboneUseCRISPRAvailable SinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMOD_C'0000a
Plasmid#127226PurposeEmpty Backbone for golden gate cloning compatible with Cermak., et al Toolkit.DepositorTypeEmpty backboneExpressionPlantAvailable SinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
EKv413(Gold Cas-guide insert4 2 gene version)
Plasmid#248342PurposegRNA insert vector for Golden Gate Assembly, 2 gene or 4 gRNAs versionDepositorTypeEmpty backboneUseCRISPRAvailable SinceJan. 6, 2026AvailabilityAcademic Institutions and Nonprofits only -
EKv414(Gold Cas-guide insert6 3 gene version)
Plasmid#248343PurposegRNA insert vector for Golden Gate Assembly, 3 gene or 6 gRNAs versionDepositorTypeEmpty backboneUseCRISPRAvailable SinceJan. 6, 2026AvailabilityAcademic Institutions and Nonprofits only -
EKv376(gRNAscaffold-tRNA for cloning)
Plasmid#248333PurposePlasmid contains gRNA scaffold-tRNA and is used to amplify fragments for Golden Gate assemblyDepositorInsertgRNAscaffold-tRNA
UseCRISPRAvailable SinceJan. 5, 2026AvailabilityAcademic Institutions and Nonprofits only -
pYPQ136A
Plasmid#69278PurposeGolden Gate entry vector; 6th gRNA under AtU6 promoterDepositorTypeEmpty backboneUseCRISPRAvailable SinceSept. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
IBMc460
Plasmid#161940PurposeCarries Golden Gate substitution insert for IBM with gp41-1 intein. PPhlF as C-lobe promoterDepositorInsertpSB1A3-BbsI-GY-gp-41-1N-cat-PPhlF-RiboJ-B32-gp41-1C-SS-SapI
ExpressionBacterialPromoterPPhlFAvailable SinceMarch 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2369 - intron GG1 - 250bp
Plasmid#159877PurposeGolden Gate compatible intron with PATCs for reduced germline silencingDepositorInsertintron GG1 - 250bp
ExpressionWormMutationNot applicableAvailable SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMOD_D0000a
Plasmid#127230PurposeEmpty Backbone for golden gate cloning compatible with Cermak., et al Toolkit.DepositorTypeEmpty backboneExpressionPlantAvailable SinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pICH47742:FCP:Cas9YFP
Plasmid#85986PurposeGolden Gate Level 1 cassette encoding a Cas9-YFP fusion under the FCP promoterDepositorInsertCas9
UseCRISPR; Golden gate assemblyTagsSV40 NLS, YFPPromoterFCPAvailable SinceJan. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
sgRNA_expression_vector
Plasmid#210212PurposeAn empty gRNA expression vector to clone U6 promoter driven sgRNAs with gRNA scaffold and with co-expression of DsRed. sgRNA can be cloned by Golden Gate Assembly or restriction digestion using BbsI.DepositorTypeEmpty backboneExpressionBacterial and MammalianPromoterU6Available SinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only