1. p416 103Q GAL

   Plasmid

   #1186

   Depositor

   Susan Lindquist

   Article

   Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

   Insert

   htt 103Q (HTT Human)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

These results may also be relevant.

1. BS-TRP1-PGAL-GFP-Cps1

   Plasmid

   #207030

   Purpose

   Expression of GFP-Cps1 under GAL promoter.

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   Cps1
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   June 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pJET-TtrpC

   Plasmid

   #213467

   Purpose

   Vector containing the TrpC terminator for Golden Gate cloning in the Fg vector

   Depositor

   Kim Hammond-Kosack

   Article

   Darino et al Fungal Biol Biotechnol. 2024 Feb 26;11(1

   Insert

   Terminator of tryptophan synthase of Aspergillus nidulans
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pJET-PgpdA

   Plasmid

   #213466

   Purpose

   Vector containing the gpdA promoter for Golden Gate cloning in the Fg vector

   Depositor

   Kim Hammond-Kosack

   Article

   Darino et al Fungal Biol Biotechnol. 2024 Feb 26;11(1

   Insert

   Promoter of the glyceraldehyde-3-phosphate dehydrogenase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pJET-PtrpC

   Plasmid

   #213465

   Purpose

   Vector containing the TrpC promoter for Golden Gate cloning in the Fg vector

   Depositor

   Kim Hammond-Kosack

   Article

   Darino et al Fungal Biol Biotechnol. 2024 Feb 26;11(1

   Insert

   Promoter of tryptophan synthase of Aspergillus nidulans
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3.1-Hyg-nCas9n

   Plasmid

   #217487

   Purpose

   Expression of nuclear nCas9n

   Depositor

   Bertrand Collet

   Article

   Chaumont et al BMC Genomics. 2024 Jun 29;25(1):650. doi

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. P1K-HFL1-8flag-LEU

   Plasmid

   #207035

   Purpose

   Expression of Hfl1-8Flag. Uses auxotrophic marker LEU2(Saccharomyces cerevisiae).

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   HFL1
   

   Use

   Tags

   8xFLAG

   Expression

   Yeast

   Mutation

   Promoter

   pHFL1

   Available since

   March 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. PCUP1-4V5-Pho8-Met

   Plasmid

   #207029

   Purpose

   Expression of 4V5-Pho8 under CPU1 promoter.

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   Pho8
   

   Use

   Tags

   4xV5

   Expression

   Yeast

   Mutation

   Promoter

   pCUP1

   Available since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. BS-Trp-PCUP1-4myc-Pho8

   Plasmid

   #207028

   Purpose

   Expression of 4Myc-Pho8 under CPU1 promoter.

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   Pho8
   

   Use

   Tags

   4xMyc

   Expression

   Yeast

   Mutation

   Promoter

   pCUP1

   Available since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. PCUP1-Vac8-GFP-Ura

   Plasmid

   #207027

   Purpose

   Expression of Vac8-GFP under CPU1 promoter.

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   Vac8
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   pCUP1

   Available since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. PCUP1-GFP-Vma13-Ura

    Plasmid

    #207026

    Purpose

    Expression of GFP-Vma13 under CPU1 promoter.

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    Vma13 ORF
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    pCUP1

    Available since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pKERep

    Plasmid

    #211826

    Purpose

    Medium copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein.

    Depositor

    Anita Emmerstorfer-Augustin

    Article

    Arhar et al Microb Cell Fact. 2024 Jan 3;23(1):9. do

    Insert

    eGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    Feb. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. P1K-3HA-Atg8∆GR-MET15

    Plasmid

    #207033

    Purpose

    Expression of 3HA-Atg8∆GR. This variant cannot be conjugated to PE.

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    ATG8
    

    Use

    Tags

    3xHA

    Expression

    Yeast

    Mutation

    ∆GR

    Promoter

    pATG8

    Available since

    Feb. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. PCUP1-mRuby3-Pho8-406

    Plasmid

    #207043

    Purpose

    Expression of mRuby3-Pho8 under CPU1 promoter.

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    PHO8
    

    Use

    Tags

    mRuby3

    Expression

    Yeast

    Mutation

    Promoter

    pCUP1

    Available since

    Feb. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pKESa

    Plasmid

    #211828

    Purpose

    Low copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein.

    Depositor

    Anita Emmerstorfer-Augustin

    Article

    Arhar et al Microb Cell Fact. 2024 Jan 3;23(1):9. do

    Insert

    eGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    Feb. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJR152

    Plasmid

    #196280

    Purpose

    Cassette used in dual sgRNA library generation

    Depositor

    Rebecca Voorhees

    Article

    Guna et al BMC Genomics. 2023 Oct 30;24(1):651. doi

    Insert

    hU6-sgRNA-CR3
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDGE1176

    Plasmid

    #202774

    Purpose

    CRISPR-KO in Arabidopsis thaliana

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant Methods. 2023 Mar 28;19(1):30. doi

    Inserts

    FAST marker
    pRPS5a:zCas9_tNbEU
    ccdB cassette (BsaI-excisable)
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDGE1177

    Plasmid

    #202775

    Purpose

    CRISPR-KO with TRE2 exonuclease co-expression in Arabidopsis thaliana

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant Methods. 2023 Mar 28;19(1):30. doi

    Inserts

    FAST marker
    pRPS5a:TREX2-zCas9_tNbEU
    ccdB cassette (BsaI-excisable)
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRA1EGFP

    Plasmid

    #193791

    Purpose

    Expresses EGFP under the control of PR promoter, pUC origin of replication, Ampicillin selection

    Depositor

    Sangram Bagh

    Article

    Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PR promoter

    Available since

    April 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. UG75-PAH1-3HA

    Plasmid

    #184761

    Purpose

    PAH1 overexpression. Promotes the formation of diacylglycerol from phosphatidate. Uses antibiotic resistance marker hphMX6.

    Depositor

    Zhiping Xie

    Article

    Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

    Insert

    PAH1 (PAH1 Budding Yeast)
    

    Use

    Tags

    3HA

    Expression

    Yeast

    Mutation

    Promoter

    PAH1

    Available since

    Feb. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. UG75-CDS1-3HA

    Plasmid

    #184760

    Purpose

    CDS1 overexpression. Diverts glycerolipid synthesis pathway towards phospholipids. Uses antibiotic resistance marker hphMX6.

    Depositor

    Zhiping Xie

    Article

    Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

    Insert

    CDS1 (CDS1 Budding Yeast)
    

    Use

    Tags

    3HA

    Expression

    Yeast

    Mutation

    Promoter

    CDS1

    Available since

    Feb. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge