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Plasmid#166712Purposeexpresses AMOT with inactivating mutation in second PPxY motif in mammalian cellsDepositorInsertAngiomotin p130 P262A P263A (AMOT Human)
UseTags2xHAExpressionMammalianMutationchanged proline 262 to alanine, proline 263 to al…PromoterCMV promoterAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3HA-AMOT p130∆PPxY3
Plasmid#166713Purposeexpresses AMOT with inactivating mutation in third PPxY motif in mammalian cellsDepositorInsertAngiomotin p130 P3072A P308A (AMOT Human)
UseTags2xHAExpressionMammalianMutationchanged proline 307 to alanine, proline 308 to al…PromoterCMV promoterAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3HA-AMOT p130∆PPxY1(E104K/E105K)
Plasmid#171130Purposeexpresses AMOT(E104K/E105K) in mammalian cellsDepositorInsertAngiomotin p130 E104K/E105K (AMOT Human)
UseTags2xHAExpressionMammalianMutationchanged glutamic acid residues 104 and 105 to lys…PromoterAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET15b p97-Strep-His E314Amb
Plasmid#169021PurposeExpresses p97-Strep-His E314Amb (incorporates noncanonical amino acid at position 314 via amber suppression) in bacteriaDepositorInsertp97
UseTagsHis and SBPExpressionBacterialMutationE314TAGPromoterT7Available sinceJuly 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
Epac-S-H90
Plasmid#170340PurposeCFP(UST)_EPACdDEPCD_cp173Venus(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.DepositorInsertCFP(UST)_EPACdDEPCD_cp173Venus(ST) (RAPGEF3 Human)
UseTagsExpressionMammalianMutationDeletion of DEP domain, Catalytically dead T781A …PromoterCMVAvailable sinceJune 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDH_CMV_HMGB_ctail_mKate2_CLEAVAGE
Plasmid#167338PurposeExpresses mKate2 fused to TFAM HMGB+C-tail mutant in mammalian cellsDepositorInsertTFAM (TFAM Human)
UseLentiviralTagsmKate2ExpressionMammalianMutationTruncation mutant containing MTS (1-42) as well a…PromoterCMVAvailable sinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
deltaVP1 + VP2C-MIOX
Plasmid#166680PurposeYeast integrative plasmid for expressing deltaVP1 (GAL1 promoter) and mouse myo-inositol oxygenase tagged with VP2C (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertsVP2C-MIOX
Murine polyomavirus deltaVP1 (NLS deletion mutant)
UseSynthetic BiologyTagsExpressionYeastMutationPromoterGAL1 and GAL10Available sinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
wtVP1 + VP2C-GFP
Plasmid#166674PurposeYeast integrative plasmid for expressing wild-type Murine polyomavirus VP1 (GAL1 promoter) and GFP tagged with VP2C (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertsVP2C-GFP
Wild-type Murine polyomavirus VP1
UseSynthetic BiologyTagsExpressionYeastMutationPromoterGAL1 and GAL10Available sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT 3xFLAG-MAVS-Gln93
Plasmid#158629PurposeGateway entry vector for an inducible 3xFLAG-tagged MAVS mutantDepositorInsertMAVS (MAVS Human)
UseGateway entry vectorTags3XFLAGExpressionMutationE93QPromoterAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT 3xFLAG-MAVS-Ala148
Plasmid#158630PurposeGateway entry vector for an inducible 3xFLAG-tagged MAVS mutantDepositorInsertMAVS (MAVS Human)
UseGateway entry vectorTags3XFLAGExpressionMutationQ148APromoterAvailable sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-FRT-TO-Nprot_GC3opt
Plasmid#157730Purposemammalian expression of untagged SARS-CoV-2 N protein under control of a tetracycline-inducible promoterDepositorInsertNprot_GC3opt (N SARS-CoV-2)
UseTagsExpressionMammalianMutationcodon optimized; gene expression was optimized by…PromoterAvailable sinceAug. 6, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA5-FRT-TO-Nsp8_GC3opt
Plasmid#157697Purposemammalian expression of untagged SARS-CoV-2 Nsp8 under control of a tetracycline-inducible promoterDepositorInsertNsp8_GC3opt (ORF1ab SARS-CoV-2)
UseTagsExpressionMammalianMutationcodon optimized; gene expression was optimized by…PromoterAvailable sinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA-FRT-TO-Nsp8-Nsp7_GC3opt
Plasmid#157700Purposemammalian expression of untagged SARS-CoV-2 Nsp8-Nsp7 fusion protein under control of a tetracycline-inducible promoterDepositorInsertNsp8-Nsp7_GC3opt (ORF1ab SARS-CoV-2)
UseTagsExpressionMammalianMutationcodon optimized; gene expression was optimized by…PromoterAvailable sinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA5-FRT-TO-FH-Nsp8_GC3opt
Plasmid#157698Purposemammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp8 under control of a tetracycline-inducible promoterDepositorInsertFH-Nsp8_GC3opt (ORF1ab SARS-CoV-2)
UseTagsFlag-His8ExpressionMammalianMutationcodon optimized; gene expression was optimized by…PromoterAvailable sinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGEMHE:mTREK-1_cryst
Plasmid#133271PurposeX. laevis expression vector. It will generate the TREK-1 channelDepositorInsertPotassium channel subfamily K member 2 (KCNK2, TREK-1) (Kcnk2 )
UseOocyte expressionTagsExpressionMutationaa 21-322, K84R, Q85E, T86K, I88L, A89R, Q90A, A9…PromoterAvailable sinceApril 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEBTetDBl-CLIP-Cep76
Plasmid#136863PurposeMammalian expression of the centrosomal protein Cep76 N-terminally fused to CLIP-tagDepositorInsertCLIP-Cep76 (CEP76 Synthetic, Human)
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceMarch 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEBTetDBl-CLIP-Cep63
Plasmid#136861PurposeMammalian expression of the centrosomal protein Cep63 N-terminally fused to CLIP-tagDepositorInsertCLIP-Cep63 (CEP63 Synthetic, Human)
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceMarch 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEBTetDBl-CLIP-Cep27
Plasmid#136858PurposeMammalian expression of the centrosomal protein Cep27 N-terminally fused to CLIP-tagDepositorInsertCLIP-Cep27 (HAUS2 Synthetic, Human)
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceMarch 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEBTetBl-CLIP-Cep76
Plasmid#136862PurposeMammalian expression of the centrosomal protein Cep76 N-terminally fused to CLIP-tagDepositorInsertCLIP-Cep76 (CEP76 Synthetic, Human)
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEBTetBl-CLIP-Cep63
Plasmid#136860PurposeMammalian expression of the centrosomal protein Cep63 N-terminally fused to CLIP-tagDepositorInsertCLIP-Cep63 (CEP63 Synthetic, Human)
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only