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pDRF1-GW ymVenus

Plasmid

#118454

Purpose

Overexpression of yeast codon-optimized mVenus

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

ymVenus

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-eEF2K

Plasmid

#110160

Purpose

Expression of human eEF2K in mammalian cells

Depositor

Daniele Guardavaccaro

Article

Kruiswijk et al Sci Signal. 2012 Jun 5;5(227):ra40. doi:

Insert

eEF2K (eukaryotic elongation factor 2 kinase) (EEF2K Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 18, 2018

Availability

Academic Institutions and Nonprofits only

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pDIRECT_23A

Plasmid

#91138

Purpose

Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: 2x35S:bar

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Engineering Reagent: 35S:AtCas9 + AtU6:gRNA

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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RF12

Bacterial Strain

#62077

Purpose

BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. trpA trpB genes deleted.

Depositor

Robert Gennis, Toshio Iwasaki

Article

Lin et al Methods Enzymol. 2015;565:45-66. doi: 10

Bacterial Resistance

None

Available since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
pTol2pA2-ubb:secHsa.ANNEXINV-mVenus, cryaa:RFP

Plasmid

#92388

Purpose

Cell death reporter

Depositor

Nicholas Cole

Article

Morsch et al Front Cell Neurosci. 2015 Aug 31;9:321.

Insert

ANNEXINV (ANXA5 Human)

Use

Zebrafish expression

Tags

mVenus

Expression

Mutation

Promoter

ubb

Available since

July 6, 2017

Availability

Academic Institutions and Nonprofits only

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GluA1 (px330:2-guideCas9)

Plasmid

#169417

Purpose

Expression of Cas9 and 2 guides

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

spCas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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pDESTmycIGF2BP3

Plasmid

#19879

Depositor

Thomas Tuschl

Article

Landthaler et al RNA. 2008 Oct 31. ():.

Insert

Insulin-like growth factor 2 mRNA binding protein 3 (IGF2BP3 Human)

Use

Tags

MYC

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 31, 2008

Availability

Academic Institutions and Nonprofits only

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HPV16 E7 TCR

Plasmid

#122728

Purpose

Express HPV16 E7 TCR

Depositor

Christian Hinrichs

Article

Jin et al JCI Insight. 2018 Apr 19;3(8). pii: 9948

Insert

HPV16 E7 TCR

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

April 12, 2019

Availability

Academic Institutions and Nonprofits only

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pMyBADC-kan

Plasmid

#84689

Purpose

Protein expression in mycobacteria

Depositor

Matthias Wilmanns

Article

Beckham et al Protein Sci. 2020 Oct 2. doi: 10.1002/pr

Type

Empty backbone

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

AraC

Available since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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qTAG-C-dTAG-Blast

Plasmid

#207716

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a dTAG-2A-Blast cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pSFVCs-lacZ

Plasmid

#92076

Purpose

This is a high level expression plasmid for production of recombinant genomic SFV RNA encoding a C-beta galactosidase fusion protein.

Depositor

Henrik Garoff

Article

Sjoberg et al Biotechnology (N Y). 1994 Nov;12(11):112

Insert

C-lacZ

Use

C-lacz expression plasmid, for run-off transcript…

Tags

Expression

Mutation

Promoter

Available since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

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pLV-SARS-CoV-2 5'UTR-Luciferase

Plasmid

#191480

Purpose

Luciferase reporter for SARS-CoV-2 5' UTR

Depositor

Hao Wu

Article

Vora et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

Insert

SARS-CoV-2 5' UTR

Use

Lentiviral and Luciferase

Tags

Expression

Mutation

contains SARS-CoV-2 5' UTR

Promoter

Available since

Nov. 15, 2022

Availability

Academic Institutions and Nonprofits only

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SpyTag-sfGFP

Plasmid

#184226

Purpose

Expresses SpyTag-superfolder GFP in the bacterial cytoplasm. SpyTag enables covalent reaction with SpyCatcher.

Depositor

Mark Howarth

Article

Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:

Insert

SpyTag-sfGFP

Use

Tags

His6, SpyTag, and TEV protease cleavage site

Expression

Bacterial

Mutation

Promoter

T5

Available since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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pEMS1982

Plasmid

#49113

Purpose

AAV plasmid with CLDN5 (Ple261) promoter driving expression of iCre.

Depositor

Elizabeth Simpson

Article

Baumann et al Exp Eye Res. 2019 Jul 16:107728. doi: 10

Insert

ssAAV-Ple261-iCre

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CLDN5

Available since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pMiniT:SEP-GluA1 TKIT donor

Plasmid

#169418

Purpose

Donor DNA for TKIT knockin of SEP to GluA1 in mouse

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

Super ecliptic pHluorin (SEP)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pFF753

Plasmid

#61453

Purpose

IPTG-inducible msd(wt)

Depositor

Timothy Lu

Article

Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

Insert

msd(wt)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

pLacO

Available since

Feb. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pTXB1-pA-M.EcoGII

Plasmid

#192873

Purpose

pA-M.EcoGII expression plasmid, originally created for BIND&MODIFY method, a Long-range single-molecule mapping of chromatin modification in eukaryotes. doi: https://doi.org/10.1101/2021.07.08.451578

Depositor

Chong Tang

Article

Weng et al Genome Biol. 2023 Mar 29;24(1):61. doi:

Insert

pA-M.EcoGII

Use

Tags

3X Flag Tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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mito-EcSTH-LbNOX

Plasmid

#211922

Purpose

Mitochondrial localization of EcSTH-LbNOX

Depositor

Binghui Li

Article

Yang et al Nat Commun. 2022 Nov 17;13(1):7031. doi:

Insert

mito-EcSTH-LbNOX

Use

Lentiviral

Tags

3xFLAG

Expression

Mutation

Promoter

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pVITRO-HPV56 L1L2

Plasmid

#52585

Purpose

Expresses HPV56 L1 and L2 in mammalian cells

Depositor

Richard Roden

Article

Kwak et al PLoS One. 2014 May 9;9(5):e97232. doi: 1

Inserts

HPV56 L1
HPV56 L2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 21, 2014

Availability

Academic Institutions and Nonprofits only

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pJAT56

Plasmid

#204300

Purpose

FExpress phiC31 integrase under heat shock control. Insert marked with 3XP3-EGFP, which expresses in eyes.

Depositor

David Stern

Article

Stern et al bioRxiv 2023.06.17.545412

Insert

3XP3-EGFP

Use

CRISPR

Tags

Expression

Mutation

Promoter

3XP3

Available since

Aug. 29, 2023

Availability

Academic Institutions and Nonprofits only

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