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pET-3c-rhVTN_FL

Plasmid

#30223

Depositor

James Thomson

Article

Chen et al Nat Methods. 2011 Apr 10. ():.

Insert

rhVitronectin without deletions (VTN Human)

Use

Tags

Expression

Bacterial

Mutation

No deletions

Promoter

Available since

May 24, 2011

Availability

Academic Institutions and Nonprofits only

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pLVX-CMV-Galpha-i3-IRESWT-Gbetagamma ONE-GO

Plasmid

#204341

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Insert

Galpha-i3/Gbetagamma ONE-GO

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-Tspan4

Plasmid

#210323

Purpose

Expresses Tetraspanin-4 (TSPAN4) as migrasome marker

Depositor

Li Yu

Article

Ma et al Cell Res. 2015 Jan;25(1):24-38. doi: 10.

Insert

Tetraspanin 4 (Tspan4 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pNIC28-DENV3-NS5FL

Plasmid

#86013

Purpose

Expression of dengue virus 3 NS5 protein

Depositor

Dahai Luo

Article

Zhao et al PLoS Pathog. 2015 Mar 16;11(3):e1004682.

Insert

DENV3-NS5FL

Use

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

amino acid residues 6 to 895 of DENV3 NS5

Promoter

T7

Available since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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LEPG

Plasmid

#111160

Purpose

miR-E (miR-30 variant)-based RNAi with GFP reporter

Depositor

Johannes Zuber

Article

Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

Insert

miR-E (miR-30 variant)

Use

Retroviral

Tags

Expression

Mutation

WT

Promoter

Available since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

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pTO_CHMP3_LAP

Plasmid

#101849

Purpose

Tet-inducible expression of human CHMP3 with a C-terminal GFP and long linker (LAP tag), compatible with Flp-In recombination for stable integration

Depositor

Daniel Gerlich

Article

Mierzwa et al Nat Cell Biol. 2017 Jul;19(7):787-798. d

Insert

Charged Multivesicular Body Protein 3 (CHMP3 Human)

Use

Gateway cloning, flp-frt recombination, tet-induc…

Tags

Localization and Affinity Purification (LAP) tag

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Available since

Dec. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pSecTag-ATX

Plasmid

#17839

Depositor

Steven Rosen

Article

Kanda et al Nat Immunol. 2008 Apr . 9(4):415-23.

Insert

Autotaxin (ENPP2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 29, 2008

Availability

Academic Institutions and Nonprofits only
mEmerald-KLC3-N-18

Plasmid

#62760

Purpose

Localization: Kinesin Light Chain , N1 Cloning Vector, Excitation:487, Emission:509

Depositor

Michael Davidson

Insert

KLC3 (Klc3 Mouse)

Use

Tags

mEmerald

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

March 10, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pNCS mOrange

Plasmid

#91764

Purpose

Bacterial expression of a fluorescent protein, monomeric Orange (mOrange)

Depositor

Roger Tsien, Erik Rodriguez

Article

Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

Insert

monomeric Orange

Use

Tags

Hexa-Histidine tag, Xpress epitope for detection …

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 9, 2017

Availability

Academic Institutions and Nonprofits only

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FUW-tetO-hMYC

Plasmid

#20723

Depositor

Rudolf Jaenisch

Article

Hockemeyer et al Cell Stem Cell. 2008 Sep 11. 3(3):346-53

Insert

c-MYC (MYC Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2009

Availability

Academic Institutions and Nonprofits only

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pJEC819

Plasmid

#190815

Purpose

CRISPRa backbone plasmid harboring BbsI sites for easy cloning of sgRNA targeting regions via Golden gate.

Depositor

James Chappell

Article

Ameruoso et al Nucleic Acids Res. 2022 Jul 22;50(13):77

Insert

dCas9-XTEN-rpoA

Use

CRISPR and Synthetic Biology

Tags

XTEN (linker), followed by the N-terminal domain …

Expression

Mutation

Promoter

SP30

Available since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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GST-TEV-FUS

Plasmid

#29629

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS (FUS Human)

Use

Tags

GST and TEV

Expression

Bacterial

Mutation

Promoter

Available since

July 18, 2011

Availability

Academic Institutions and Nonprofits only

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pHR EGFR-CD3ε-FusionRed

Plasmid

#188626

Purpose

Human EGFR labeled with CD3ε ITAMs for monitoring with CD3ε/ZtSH2 pYtag biosensor

Depositor

Jared Toettcher

Article

Farahani et al Elife. 2023 May 22;12:e82863. doi: 10.75

Insert

EGFR (EGFR Synthetic, Human)

Use

Lentiviral

Tags

CD3ε-FusionRed

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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FKBP-beta1(617-949)-GFP

Plasmid

#100732

Purpose

To express a clathrin "hook" in mammalian cells to hot-wire endocytosis

Depositor

Stephen Royle

Article

Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

Insert

AP1B1 (AP1B1 Human)

Use

Tags

FKBP and GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pHAGE-EFS-PCP-GFPnls

Plasmid

#121938

Purpose

CRISPR-Sirius plasmid

Depositor

Thoru Pederson

Article

Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

Insert

PCP-GFPnls

Use

Lentiviral

Tags

sfGFP

Expression

Mammalian

Mutation

Promoter

EFS

Available since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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CRISPR-SB

Plasmid

#177936

Purpose

Expresses SpCas9 and sgRNA. Can be integrated into the genome upon co-delivery of Sleeping Beauty transposase.

Depositor

Roland Rad

Article

Kaltenbacher et al Nat Protoc. 2022 Apr;17(4):1142-1188. do

Type

Empty backbone

Use

CRISPR; Transposon

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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PA-GFPmt

Plasmid

#19989

Depositor

Orian Shirihai

Article

Twig et al Am J Physiol Cell Physiol. 2006 Jul . 29

Insert

GFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 17, 2009

Availability

Academic Institutions and Nonprofits only
pHSS6hsILMi20

Plasmid

#102536

Purpose

Helper plasmid expressing Minos transposase mRNA (Drosophila hsp70 promoter)

Depositor

Michalis Averof, Charalambos Savakis

Article

Klinakis et al Insect Mol Biol. 2000 Jun;9(3):269-75.

Insert

Minos transposase

Use

Tags

Expression

Mutation

Promoter

Available since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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pCL20C-ddIPpoI

Plasmid

#49053

Purpose

Expresses the restriction enzyme IPpoI under the control of Shield1

Depositor

Michael Kastan

Article

Goldstein et al Proc Natl Acad Sci U S A. 2013 Sep 30.

Insert

DD-HA-ER-IPpoI

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Dec. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1-14-3-3 gamma GST

Plasmid

#13280

Depositor

Michael Yaffe

Article

Rittinger et al Mol Cell. 1999 Aug . 4(2):153-66.

Insert

14-3-3 gamma (Ywhag Rat)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 7, 2006

Availability

Academic Institutions and Nonprofits only