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Plasmid#134590PurposeLow Phosphate inducible mdlC benzylformate decarboxylaseDepositorInsertmdlC (benzylformate decarboxylase) C -terminal his tag
UseTags6x-HisExpressionBacterialMutationPromoteryibDpAvailable sinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pACYC-Skp-hLYZ
Plasmid#83924PurposeCo-expresses cytoplasmic copies of the Skp protein folding chaperone and wild type human lysozyme..DepositorInsertsUseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSB1A2QS
Plasmid#138268PurposeMINPA Gene circuit expression in E. coli. GFP serves as a reporter for Plux/tet promoter activity. mCherry serves as reporter for Para/lac promoter activityDepositorInsertsAraC-TetR operon
LuxR-LacI operon
mCherry
GFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPara/lac and Plux/tetAvailable sinceApril 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAW-EGFP-4xlet-7 (pAW-AS27)
Plasmid#40834PurposemiRNA sensor used in Drosophila cell linesDepositorInsertFour target sites perfectly complementary to let-7
UseMirna reporterTagsEGFPExpressionInsectMutationPromoterActin5CAvailable sinceSept. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
GFP Snail 4SA
Plasmid#16227DepositorInsertSnail (SNAI1 Human)
UseTagsEGFPExpressionMammalianMutation4SA: S108A, S112A, S116A, S120APromoterAvailable sinceNov. 30, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAP1837
Plasmid#137086PurposePlasmid for PCR-generated donor DNA for rapid tagging of proteins with mCherryDepositorInsertmCherry
UsePcr template for donor dnaTagsExpressionBacterialMutationPromoterAvailable sinceMarch 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAP3041
Plasmid#137083PurposePlasmid for PCR-generated donor DNA for rapid tagging of proteins with GFP11-3xFlag-tagRFPDepositorInsertGFP11::3xFlag::tagRFP
UsePcr template for donor dnaTagsExpressionBacterialMutationPromoterAvailable sinceMarch 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZF-BCB-GCN
Plasmid#127476Purposecoding sequence for synthetic zinc finger ZF-BCB-GCNDepositorInsertZF-BCB synthetic transcription factor fused with GCN4 leucine zipper
UseCell-free transcription–translationTagsGCN4 leucine zipperExpressionMutationPromoterT7Available sinceJuly 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBRC_double_attB_GFP_donor
Plasmid#169692Purposecargo vector for the phiC31 system with Psur5:GFP, cb_unc-119, and Gateway R1R2 site (for gene of interest insertion)DepositorTypeEmpty backboneUseTagsExpressionWormMutationPromoterAvailable sinceOct. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
mEos2-Talin-IBS2-7
Plasmid#57424PurposeLocalization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581DepositorInsertTalin IBS2 (Tln1 Mouse)
UseTagsmEos2ExpressionMammalianMutationTalin Integrin Binding SitePromoterCMVAvailable sinceFeb. 5, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLITMUS-rpoN-cI4A5C6G7G-J23106-geneIII
Plasmid#80909PurposePhagemid (encodes cI4A5C6G7G and M13 gene III)DepositorInsertsM13 gene III
cI4A5C6G7G
UseTagsExpressionBacterialMutation238G to 238D (please see depositor comment below)PromoterBBa_J23106 and rpoNAvailable sinceDec. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCVL SFFV Sbf-Sal linker
Plasmid#50434PurposeCloning vector for coexpression of gene cloned in between Sbf and Sal sites with BFP in mammalian cells. Made for cloning of meganuclease for direct comparison with megaTALs (pCVL TAL Xba-Sal).DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterSFFVAvailable sinceMay 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pMOD_A0301
Plasmid#91005PurposeModule A, Promoter: 35S, Gene: AtCas9_H840A nickase, Terminator: HSPDepositorInsertAtCas9_H840A nickase
UseCRISPRTagsExpressionMutationH840APromoter35SAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
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SlutrCas9
Plasmid#163795PurposeExpresses SlutrCas9, and cloning backbone for sgRNADepositorTypeEmpty backboneUseAAV and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
ShaCas9
Plasmid#163794PurposeExpresses ShaCas9, and cloning backbone for sgRNADepositorTypeEmpty backboneUseAAV and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOVC1_ScPH1-S_X
Plasmid#131184PurposeExpresses ScPH1-S (Synechosystis PCC6803) followed by restriction sitesDepositorInsertScPH1-S
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
CMV-2xFLAG-Atoh1ER
Plasmid#101736PurposeExpresses mouse Atoh1 fused to ERDepositorInsertAtoh1 (Atoh1 Mouse)
UseTags2x FLAG and ERExpressionMammalianMutationPromoterAvailable sinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR2KN0040
Plasmid#117607PurposeLevel2 MoClo construct, containing level1 plant expression cassettes for SaCas9(pEPOR1CB0015) and sgRNA_NbPDS(pEPOR1CB0058)DepositorInsert[35S:SaCas9(pEPOR1CB0015) ] +[AtU6-26:sgRNA]
UseTagsExpressionPlantMutationPromoterAvailable sinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only