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pMXs-hs-3xHA-7CtoA-LIN-41

Plasmid

#52726

Purpose

retroviral expression of human LIN-41 / TRIM71 containing cysteine to alanine point mutations of all 7 cysteines in the RING domain and 3 N-terminal HA tags

Depositor

Shinya Yamanaka

Article

Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

Insert

LIN-41 (TRIM71 Human)

Use

Retroviral

Tags

3xHA

Expression

Mammalian

Mutation

changed cysteines 12, 15, 61, 66, 69, 91, and 94 …

Promoter

Available since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

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pLKO-tet-puro-sh-ex16b

Plasmid

#35168

Depositor

Benjamin Blencowe

Article

Gabut et al Cell. 2011 Sep 30;147(1):132-46. Epub 20

Insert

Foxp1 (Foxp1 Mouse)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
FRT3-Mb2-HA-mTfp1-2A (IG155)

Plasmid

#99621

Purpose

To clone gene of interest downstream of FRT3-Mb2-HA-mTfp1-2A

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

Mb2-HA-mTfp1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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FRT1-Mb2YFP-2A (IG153)

Plasmid

#99619

Purpose

To clone gene of interest downstream of FRT1-Mb2YFP-2A cassette

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

Mb2EYFP

Use

Tags

T2A

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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FRT2-Mb2Tomato-2A (IG154)

Plasmid

#99620

Purpose

To clone gene of interest downstream of FRT2-Mb2Tomato-2A cassette

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

Mb2Tomato

Use

Tags

T2A

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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FRT3-HA-H2B-Cerulean-2A (IG158))

Plasmid

#99624

Purpose

To clone gene of interest downstream of FRT3-HA-H2B-Cerulean-2A cassette

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

HA-H2B-Cerulean

Use

Tags

T2A

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR-2B-Nkx2-5 delta HD-G8

Plasmid

#98614

Purpose

Gateway entry vector NKX2-5 _HD

Depositor

Richard Harvey

Article

Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

Insert

NKX2-5 _HD (Nkx2-5 Mouse)

Use

Entry vector

Tags

Expression

Mutation

Deletion of the homeodomain. Replaced by 8 glycin…

Promoter

Available since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pCIG AKT3 E17K;K177M

Plasmid

#73049

Purpose

Human AKT3 E17K;K177M

Depositor

Joseph Gleeson

Article

Baek et al Nat Med. 2015 Dec;21(12):1445-54. doi: 1

Insert

AKT3 E17K;K177M (AKT3 Human)

Use

Tags

Expression

Mammalian

Mutation

E17K;K177M

Promoter

Available since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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HNF4A_P2-226

Plasmid

#31064

Depositor

Gerhart Ryffel

Article

Thomas et al Hum Mol Genet. 2001 Sep 15. 10(19):2089-

Insert

HNF4A P2 promoter (HNF4A Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

-226 to -1 of P2 HNF4A promoter

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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tdEos-Keratin-17

Plasmid

#57629

Purpose

Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

Insert

Keratin (KRT18 Human)

Use

Tags

tdEos

Expression

Mammalian

Mutation

V282L

Promoter

CMV

Available since

Jan. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits
FRIG-myc-Sema4D-CD4

Plasmid

#51606

Purpose

expresses myc-tagged Sema4D/CD4 non-cleavable chimera with extracellular Sema4D, intracellular CD4 in lentiviral backbone

Depositor

Suzanne Paradis

Article

Raissi et al Mol Cell Neurosci. 2013 Nov;57:23-32. do

Insert

Sema4D/CD4-FLAG (Sema4d Mouse, Human)

Use

Lentiviral

Tags

FLAG and myc

Expression

Mammalian

Mutation

Sema4D amino acids 660-861 were replaced with CD4…

Promoter

RSV

Available since

April 7, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA5-F-Med26-K74A K75A

Plasmid

#49253

Purpose

Mammalian expression of flag-tagged human Med26-K74A K75A

Depositor

Ronald Conaway, Joan Conaway

Article

Takahashi et al Cell. 2011 Jul 8;146(1):92-104.

Insert

Med 26 (MED26 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

K74A K75A

Promoter

Available since

Dec. 5, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 T21R-F8.2(S54L+T127A)-T2A-mCherry

Plasmid

#225588

Purpose

Mammalian expression vector for TRIM21 RING-F8.2(S54L+T127A) anti-tau degrader with a self-cleaving T2A-mCherry fluorescent expression reporter

Depositor

William McEwan

Article

Benn et al Science. 2024 Aug 30;385(6712):1009-1016

Insert

T21R-F8.2(S54L+T127A)-T2A-mCherry

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA4TO T21R-F8.2 (S54L+T127A)-T2A-mCherry

Plasmid

#225584

Purpose

Mammalian expression vector for Doxycycline inducible expression of TRIM21 RING-F8.2(S54L+T127A) anti-tau degrader with a self-cleaving T2A-mCherry fluorescent expression reporter

Depositor

William McEwan

Article

Benn et al Science. 2024 Aug 30;385(6712):1009-1016

Insert

T21R-F8.2 (S54L+T127A)-T2A-mCherry

Use

Tags

mCherry

Expression

Mammalian

Mutation

T21R-F8.2 (S54L+T127A)

Promoter

CMV

Available since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pFUSE-CHIg-hG1_AT118-L-Fc LALA + PG

Plasmid

#222852

Purpose

Mammalian expression plasmid for secretion of AT118-L a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R) fused to human IgG1 Fc

Depositor

Andrew Kruse

Article

Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

Insert

AT118-L

Use

Tags

IgG1_Fc

Expression

Mammalian

Mutation

L234A, L235A, P329G

Promoter

Available since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pFUSE-CHIg-hG1_AT118-L F47T, Y98F-Fc LALA + PG

Plasmid

#222854

Purpose

Mammalian expression plasmid for secretion of a non-AT1R binding control AT118-L variant fused to human IgG1 Fc

Depositor

Andrew Kruse

Article

Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

Insert

AT118-L

Use

Tags

IgG1_Fc

Expression

Mammalian

Mutation

L234A, L235A, P329G

Promoter

Available since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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IL2-AHDC2-Cherry in pmCherryN1

Plasmid

#187291

Purpose

Express pmCherry-IL2-AH-deltaC2

Depositor

Alpha Yap

Article

Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

Insert

Scraps interleukin-2 receptor alpha-subunit, anillin (IL2, ANLN Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

AH-deltaC2

Promoter

CMV

Available since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_SBP-EGFP-CD59

Plasmid

#222307

Purpose

Synchronize the trafficking of CD59 from the ER.

Depositor

Franck Perez

Article

Fourriere et al J Cell Biol. 2019 Jul 1;218(7):2215-2231

Insert

Streptavidin-KDEL and CD59 fused to SBP-EGFP (CD59 Human)

Use

Tags

Expression

Mammalian

Mutation

signal peptide removed

Promoter

CMV

Available since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV 3xFLAG-MCRS1

Plasmid

#231241

Purpose

Flag-tagged wild-type MCRS1 over-expression construct for transient expression in mammalian cells

Depositor

Michael Rape

Article

Mark et al Cell. 2023 Aug 3;186(16):3460-3475.e23.

Insert

MCRS1 (MCRS1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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lenti_U6_miR-Oct4e_TRE12_OCT4(co)-mNeonGreen_WPRE

Plasmid

#229544

Purpose

Lentiviral construct to express synthetic miRNA and OCT4-mNeonGreen fusion protein

Depositor

Domitilla Del Vecchio

Article

Ilia et al Sci Adv. 2023 Dec;9(48):eadg8495. doi: 1

Insert

Oct4(co)-mNeonGreen (POU5F1 Human)

Use

Lentiviral

Tags

Oct4-mNeonGreen

Expression

Mammalian

Mutation

codon-optimized OCT4

Promoter

TRE12

Available since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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