-
Plasmid#168186Purposebacteria reporterDepositorInsertPBAD::tag-rfp-T PtetA::sfgfp
UseTagsExpressionBacterialMutationnonePromoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_FSCN1-Halo
Plasmid#175343PurposeProduction of HaloTag fusion proteinDepositorInsertFSCN1 (FSCN1 Human)
UseCell free expressionTagsHaloTag-6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDH013
Plasmid#157638PurposesMHT-113 fused to GFP and RFPDepositorInsertssMHT(1-113)-SYNZIP17-GFP
RFP-SYNZIP18-sMHT(114-23)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
SnFR-gamma2
Plasmid#165495PurposeGreen fluorescent glutamate reporter fused to TARP gamma-2 for mammalian expression.DepositorInsertiGluSnFR extracellular domain fused to Stargazin (gamma-2) via NETO2 TM domain (Cacng2 Synthetic, Rat)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTNT-pT7-tetR-mCherry
Plasmid#140870PurposeT7 promoter driven synthesis of TetR-mCherry fusion proteinDepositorInsertTetR-mCherry expressed with T7 promoter
UseSynthetic BiologyTagsmCherryExpressionBacterialMutationPromoterT7Available sinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-clbH-A1
Plasmid#114149PurposeExpresses ClbH-A1DepositorInsertclbH-A1
UseTags6xHis-thrombin-T7 tagExpressionBacterialMutationContains only the A1 domain of ClbHPromoterAvailable sinceAug. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
HL 1128
Bacterial Strain#60372PurposeLim Lab StrainDepositorBacterial ResistanceNoneAvailable sinceAug. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 NFAT3
Plasmid#10961DepositorInsertNFAT3 (NFATC4 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3_SARS2_JN.1
Plasmid#218678Purposeexpressing SARS-CoV-2 JN.1 spike protein for pseudovirus productionDepositorInsertSpike (JN.1)
UseTagsExpressionMammalianMutationL455S on the basis of BA.2.86PromoterCMVAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-Camk2-NES-Twitch-GR
Plasmid#173044PurposeTnC-based calcium sensor with green-red FRET pair ratiometric readoutDepositorInsertNES-Twitch-GR
UseTagsExpressionMammalianMutationPromoterCamK2Available sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDRF1-GW ytdTomato
Plasmid#118456PurposeOverexpression of yeast codon-optimized tdTomatoDepositorInsertytdTomato
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDRM18 LTN (Luciferase tdTomato Neo)
Plasmid#174721PurposeExpresses the firefly luciferase gene, E2A, tdTomato, P2A, and the neomycin resistance gene.DepositorInsertsFirefly Luciferase
tdTomato
aph
UseLentiviral and LuciferaseTagsE2A linker and P2A linkerExpressionMammalianMutationPromoterMND, MND (off Luc-E2A), and MND (off Luc-E2A-tdTo…Available sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMRBAD-Z-CGFP
Plasmid#52735PurposeL-(+)-arabinose-inducible expression of Z-CGFP positive control for in vivo split GFP assembly assayDepositorInsertZ+CGFP
UseTagsExpressionBacterialMutationPromoterPBADAvailable sinceMay 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCC_05 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dCas9-NLS-VPR-2A-Puro-WPRE
Plasmid#139090PurposeExpresses human codon-optimized inactive SpCas9 fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.DepositorInsertdSpCas9-VPR
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A, H840APromoterAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDB5320
Plasmid#204830PurposeAn integrating plasmid containing both the inducible enotetSW2 promoter and the coding sequence of the TetR repressor under the control of the constitutive CMV promoter.DepositorInsertstetR
mECitrine
UseTagsExpressionYeastMutationPromoterCMV promoter and enotetSW2 promoterAvailable sinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDB5319
Plasmid#204829PurposeAn integrating plasmid containing both the inducible enotetSW1 promoter and the coding sequence of the TetR repressor under the control of the constitutive CMV promoter.DepositorInsertstetR
mECitrine
UseTagsExpressionYeastMutationPromoterCMV promoter and enotetSW1 promoterAvailable sinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-SCP1-dSa VPR mini.-2X snRP-1 Hbb
Plasmid#99692PurposeExpresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Hbb, vector allows for strong activation of mouse Hbb, can be packaged and delivered as AAVDepositorArticleInsertdCas9 and gRNA targeting Hbb (Hbb Synthetic)
UseAAVTagsVPR miniExpressionMutationdead Cas9PromoterAvailable sinceJan. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEN395 - pCAGGS-Tir1-V5-BpA-Frt-PGK-EM7-PuroR-bpA-Frt TIGRE targeting
Plasmid#92141PurposeTargeting vector to introduce an osTir1 expressing cassette at the mouse TIGRE acceptor locus using Puromycin selection. Auxin-inducible degron system.DepositorInsertosTir1
UseMouse TargetingTagsV5 tagExpressionMammalianMutationPromoterAvailable sinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET21a-cys-His6-OtUBD
Plasmid#190091PurposeExpresses OtUBD with a non-cleavable N-terminal 6xHis tag as well as an engineered N-terminal cysteine in E. coliDepositorInsertOtUBD
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
TagBFP2-PIP4K2B
Plasmid#202728Purposeexpresses fluorescent protein-tagged enzymeDepositorInsertmTagBFP2:PIP4K2B (PIP4K2B Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only