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Plasmid#27274DepositorInsertSeptin 5 (SEPTIN5 Human)
UseTagsMyc (MASMQKLISEEDL)ExpressionMammalianMutationS327APromoterAvailable sinceJan. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 PPARG
Plasmid#67099PurposeStandard for cell-free expression benchmarking.DepositorInsertPPARG (PPARG Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCfB6681
Plasmid#106157PurposeEasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6637, integration into Yarrowia lipolytica chromosomal location IntE_3, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 PSMC1
Plasmid#67087PurposeStandard for cell-free expression benchmarking.DepositorInsertPSMC1 (PSMC1 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationE275G, I287V and R294GPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2214 - Intron GG1 - 900 bp
Plasmid#159880PurposeGolden Gate compatible intron with PATCs for reduced germline silencingDepositorInsertIntron GG1 - 900 bp
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
CAG-mCherry-CrxshRNA
Plasmid#73987PurposeCrx shRNA (mir30 backbone) was inserted into the 3'UTR of mCherry.DepositorInsertshRNA that targets the mouse Crx gene
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJH3558
Plasmid#179611PurposePunc-4::Chrimson::Cherry unc-54 3' UTR C.elegans A-MNs and others expression of Chrimson::CherryDepositorInsertChrimson
UseTagsCherryExpressionWormMutationPromoterPunc-4Available sinceMarch 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBW412hrpL-cIgfp
Plasmid#61438PurposepSB4A3 harbouring hrpL-rbs32-cI-lva-ter-Plam-rbs30-gfp-terDepositorInsertHrpL-rbs32-CI-LVA-t-plam-30gfp-t
UseTagsLVAExpressionBacterialMutationPromoterHrpLAvailable sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFUSE-B_P2rP4
Plasmid#97185PurposeFor fusion gene cloning. Recombine Gene B into this Gateway Entry VectorDepositorTypeEmpty backboneUseGatewayTagsExpressionMutationPromoterAvailable sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCA0.001
Plasmid#119557PurposeLevel 0 acceptor vector for promoters truncated to transcriptional start site with lacZ compatible with blue/white screeningDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
Ad-shMKP-3
Plasmid#131530PurposeSilence the MKP-3 expressionDepositorInsertShort hairpin mitogen-activated protein kinase phosphatase 3 (Dusp6 Mouse)
UseAdenoviralTagsNoExpressionMutationPromoterAvailable sinceNov. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCLXEBR-pTF-mBmi1
Plasmid#114317PurposeTET-inducible expression of the mBmi-1 proteinDepositorInsertmBmi1 (Bmi1 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRNA-U6-Zip let-7
Plasmid#35670DepositorInsertlet-7Zip
UseTagsExpressionMutationPromoterU6Available sinceJuly 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRNA-U6-ZipmiR122
Plasmid#35671DepositorInsertmiR-122Zip
UseTagsExpressionMutationPromoterU6Available sinceMay 7, 2012AvailabilityAcademic Institutions and Nonprofits only -
pQCi
Plasmid#154345PurposeSelf-Cutting and Integrating CRISPR backbone (px458 derivative with Cas9-intron, sgRNA, and bait integration sites)DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJuly 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pES002 (pTet-qacR wt)
Plasmid#69031Purposewild-type qacR downstream of a Tet promoterDepositorInsertqacR (qacR Synthetic)
UseTagsHis6 tagExpressionBacterialMutationPromoterpTetAvailable sinceOct. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Flag-YAP-S61A
Plasmid#33087DepositorInsertYAP (YAP1 )
UseTagsFlagExpressionMammalianMutationS61APromoterCMVAvailable sinceMarch 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
CAG-EGFP-Otx2shRNA1
Plasmid#73981PurposeOtx2 shRNA1 (mir155 backbone) was inserted into the 3'UTR of EGFP.DepositorInsertshRNA that targets the mouse Otx2 gene
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
CRISPRmTmG2
Plasmid#69992PurposeThe CRISPR construct targets near the LoxP sites in Rosa-pCA-loxP-mTdtomato-loxP-mEGFP mice.DepositorInsertgRNA that targets near LoxP sites
UseTagsExpressionMutationPromoterAvailable sinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pC0.017
Plasmid#119621PurposeLevel 0 Part. CDSDepositorInsertdCas9
UseSynthetic BiologyTagsExpressionMutationbp 331 C to A , 1237 A to C, 1284 C to T, 2121 C …PromoterAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only