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Plasmid#149393PurposeP. aeruginosa PA14 CRISPR2 locus, with 10bp addition downstream,and 7bp addition upstream of IHFprox siteDepositorInsertPseudomonas aeruginosa PA14 CRISPR2 locus
UseTagsExpressionBacterialMutation7bp inserted upstream, and 10bp inserted downstre…PromoterpBADAvailable sinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR2_+10D+10U
Plasmid#149394PurposeP. aeruginosa PA14 CRISPR2 locus, with 10bp addition downstream,and 10bp addition upstream of IHFprox siteDepositorInsertPseudomonas aeruginosa PA14 CRISPR2 locus
UseTagsExpressionBacterialMutation10bp inserted upstream and downstream of the IHFp…PromoterpBADAvailable sinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR2_IHF_Mut
Plasmid#149396PurposeP. aeruginosa PA14 CRISPR2 locus, with a mutated IHFprox site, that should not be recognized by IHFDepositorInsertPseudomonas aeruginosa PA14 CRISPR2 locus
UseTagsExpressionBacterialMutationIHFprox site is mutated at key positions needed f…PromoterpBADAvailable sinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR2_motif_scram
Plasmid#149397PurposeP. aeruginosa PA14 CRISPR2 locus, with a scrambled Upstream recognition motifDepositorInsertPseudomonas aeruginosa PA14 CRISPR2 locus
UseTagsExpressionBacterialMutationUpstream motif recognized by Cas1-2/3 during spac…PromoterpBADAvailable sinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB221
Plasmid#185071PurposeSST::ACT2 "SA236"DepositorInsertACT2
UseTagsExpressionYeastMutationACT2-SA236PromoterAvailable sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB215
Plasmid#185068PurposeSST::WAK2 EcoRI/ScaI fragment (secretion confirmed)DepositorInsertWAK2
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-eDHFR(69K6)-iRFP713
Plasmid#178855PurposeMammalian expression of eDHFR(69K6)-iRFP713DepositorInserteDHFR(69K6)-iRFP713
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJuly 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CMV:Flag-CeNL_SV40:PuroR
Plasmid#183039PurposeExpresses the FLAG-tagged CeNL (Blue Nanolantern) in mammalian cells.DepositorInsertCeNL Nanolantern
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CMV:Flag-YeNL_SV40:PuroR
Plasmid#183041PurposeExpresses the FLAG-tagged YeNL (Yellow Nanolantern) in mammalian cells.DepositorInsertYeNL Nanolantern
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CMV:Flag-OeNL_SV40:PuroR
Plasmid#183042PurposeExpresses the FLAG-tagged OeNL (Orange Nanolantern) in mammalian cells.DepositorInsertOeNL Nanolantern
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pWZL hygro-hTCAB1
Plasmid#182593Purposeexpresses hTCAB1 in mammalian cellsDepositorInserthTCAB1 (WRAP53 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmPAN3-dsRNAres_Y
Plasmid#148080PurposeInsect Expression of DmPAN3-dsRNAresDepositorInsertDmPAN3-dsRNAres (CG11486 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMSCV_Hyg_FLAG-BirA-eGFP-NLS2
Plasmid#170917PurposeExpresses FLAG-BirA-eGFP-NLS2 to be used as control in BioID or FLAG pull downDepositorInsertFLAG-BirA-eGFP-NLS2
UseTagsBirA-FLAGExpressionMammalianMutationPromoterAvailable sinceMarch 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC19_HR-BirA-FLAG_Ago2
Plasmid#170918PurposeEncodes BirA-FLAG with left and right homology arms for endogenous tagging of Ago2DepositorInsertBirA-FLAG
UseTagsExpressionMammalianMutationPromoterlacAvailable sinceMarch 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC19_HR-BirA-FLAG_Ago1
Plasmid#170919PurposeEncodes BirA-FLAG with left and right homology arms for endogenous tagging of Ago1DepositorInsertBirA-FLAG
UseTagsExpressionMammalianMutationPromoterlacAvailable sinceMarch 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCS2+WBP2NL-HA
Plasmid#174791PurposeXenopus laevis WBP2NL with 3'HA tagDepositorInsertWBP2 N-terminal like (wbp2nl.L Frog)
UseTagsHAExpressionBacterialMutationaa#165, Y to H; aa# 287, D to HPromoterSP6Available sinceDec. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pW214-lenti-sasgRNA-Esp3I-2kb-filler-pEF1s-NLS-mScarlet-I-P2A-BlastR
Plasmid#170812PurposeLentiviral vector to co-express an sasgRNA with NLS-mScarlet-IDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationNAPromoterAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
BII-gR-PnGW
Plasmid#133355PurposePiggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications), co-expressed with GFP-NLSDepositorInsertGFP-NLS
UseTagsHA-tagged GFPExpressionMammalianMutationN/APromoterCMV enhancer and hEf1a (CpG free)Available sinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTAMACHis6_PiuD
Plasmid#128953Purposeexpresses PiuD in E.coliDepositorInsertPutative outer membrane receptor for iron transport (PiuD)
UseTags6xHis and TamAExpressionBacterialMutationSignal peptide sequence has been removed (1-105)PromoterT7Available sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMH-HT_CtCHAD-H253A-R256A-R260A
Plasmid#127788PurposeExpresses a CtCHAD mutant protein in E. coli cellsDepositorInsertCtCHAD (CT0884 Chlorobium tepidum)
UseTags6xHis tag + TEV cleavage siteExpressionBacterialMutationResidues 208-522 (CHAD domain), mutations in His2…PromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only