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Addgene
Showing: 11461 - 11480 of 20580 results
  1. dCas9-CBP HAT

    Plasmid
    #179547
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human CBP HAT domain (aa 1323-1701) driven by EF-1alpha promoter
    Depositor
    Insert
    S. pyogenes dCas9 with c-terminal fusion of human CBP HAT domain (aa 1323-1701) (CREBBP Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Availability
    Academic Institutions and Nonprofits only
  2. dCas9-CBP PHD-HAT

    Plasmid
    #179548
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human CBP PHD-HAT domain (aa 1279-1701) driven by EF-1alpha promoter
    Depositor
    Insert
    S. pyogenes dCas9 with c-terminal fusion of human CBP PHD-HAT domain (aa 1279-1701) (CREBBP Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Availability
    Academic Institutions and Nonprofits only
  3. dCas9-CBP RING-PHD-HAT

    Plasmid
    #179549
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human CBP RING-PHD-HAT domain (aa 1191-1701) driven by EF-1alpha promoter
    Depositor
    Insert
    S. pyogenes dCas9 with c-terminal fusion of human CBP RING-PHD-HAT domain (aa 1191-1701) (CREBBP Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Availability
    Academic Institutions and Nonprofits only
  4. dCas9-GCN5 core

    Plasmid
    #179551
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human GCN5 core (aa 473-676) driven by EF-1alpha promoter
    Depositor
    Insert
    S. pyogenes dCas9 with c-terminal fusion of human GCN5 core (aa 473-676) (KAT2A Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Availability
    Academic Institutions and Nonprofits only
  5. pCBh_3x Flag-NLS-enOsCas12f1-NLS_pA_phU6_gRNA scaffold-spacer_pCMV_mCherry_pA

    Plasmid
    #197026
    Purpose
    Vector encoding a human codon-optimized enOsCas12f1 driven by CBh promoter, guide RNAs compatible with enOsCas12f1 driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Insert
    humanized enOsCas12f1
    Use
    Tags
    Expression
    Mammalian
    Mutation
    D52R / T132R
    Promoter
    CBh, CMV, hU6
    Availability
    Academic Institutions and Nonprofits only
  6. pCBh_3x Flag-NLS-enRhCas12f1-NLS_pA_phU6_gRNA scaffold-spacer_pCMV_mCherry_pA

    Plasmid
    #197027
    Purpose
    Vector encoding a human codon-optimized enRhCas12f1 driven by CBh promoter, guide RNAs compatible with enRhCas12f1 driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Insert
    humanized enRhCas12f1
    Use
    Tags
    Expression
    Mammalian
    Mutation
    L270R
    Promoter
    CBh, CMV, hU6
    Availability
    Academic Institutions and Nonprofits only
  7. px552-U6-gRNA1-CMV-eGFP

    Plasmid
    #211758
    Purpose
    sgRNA1 targeting Exon 1 of VEGFA gene conserved across mouse, rhesus macaque, and human.
    Depositor
    Insert
    gRNA1 targeting VEGF-A (VEGFA Human, M. mulatta (rhesus macaque), Mouse)
    Use
    AAV and CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Availability
    Academic Institutions and Nonprofits only
  8. px552-U6-gRNA2-CMV-eGFP

    Plasmid
    #211759
    Purpose
    sgRNA2 targeting Exon 1 of VEGFA gene conserved across mouse, rhesus macaque, and human.
    Depositor
    Insert
    gRNA2 targeting VEGF-A (VEGFA Human, M. mulatta (rhesus macaque), Mouse)
    Use
    AAV and CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Availability
    Academic Institutions and Nonprofits only
  9. px552-U6-gRNA1-U6-gRNA2-CMV-eGFP

    Plasmid
    #211760
    Purpose
    Paired gRNAs (sgRNA1 and 2) targeting Exon 1 of VEGFA gene conserved across mouse, rhesus macaque, and human.
    Depositor
    Insert
    gRNA1 and gRNA2 targeting VEGF-A (VEGFA Human, M. mulatta (rhesus macaque), Mouse)
    Use
    AAV and CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Availability
    Academic Institutions and Nonprofits only
Showing: 11461 - 11480 of 20580 results