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pcDNA3.1_V5-TWIST1_NEUROD1ins

Plasmid

#215036

Purpose

Mammalian expression of human TWIST1 (with insertion from NEUROD1)

Depositor

Joanna Wysocka

Article

Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

Insert

twist family bHLH transcription factor 1 (TWIST1 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

TWIST1 bHLH loop with insertion from NEUROD1 bHLH…

Promoter

CMV

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_V5-TWIST1_NEUROG2ins

Plasmid

#215037

Purpose

Mammalian expression of human TWIST1 (with insertion from NEUROG2)

Depositor

Joanna Wysocka

Article

Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

Insert

twist family bHLH transcription factor 1 (TWIST1 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

TWIST1 bHLH loop with insertion from NEUROG2 bHLH…

Promoter

CMV

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_V5-TWIST1_HAND2ins

Plasmid

#215038

Purpose

Mammalian expression of human TWIST1 (with insertion from HAND2)

Depositor

Joanna Wysocka

Article

Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

Insert

twist family bHLH transcription factor 1 (TWIST1 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

TWIST1 bHLH loop with insertion from HAND2 bHLH l…

Promoter

CMV

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_V5-TWIST1_TAL1ins

Plasmid

#215039

Purpose

Mammalian expression of human TWIST1 (with insertion from TAL1)

Depositor

Joanna Wysocka

Article

Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

Insert

twist family bHLH transcription factor 1 (TWIST1 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

TWIST1 bHLH loop with insertion from TAL1 bHLH lo…

Promoter

CMV

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_V5-TWIST1_P139S

Plasmid

#215040

Purpose

Mammalian expression of human TWIST1 (with P139S mutation)

Depositor

Joanna Wysocka

Article

Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

Insert

twist family bHLH transcription factor 1 (TWIST1 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

P139S

Promoter

CMV

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1_V5-TWIST1_L138Y

Plasmid

#215041

Purpose

Mammalian expression of human TWIST1 (with L138Y mutation)

Depositor

Joanna Wysocka

Article

Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

Insert

twist family bHLH transcription factor 1 (TWIST1 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

L138Y

Promoter

CMV

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_V5-TWIST1_L138F

Plasmid

#215042

Purpose

Mammalian expression of human TWIST1 (with L138F mutation)

Depositor

Joanna Wysocka

Article

Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

Insert

twist family bHLH transcription factor 1 (TWIST1 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

L138F

Promoter

CMV

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_FKBP-V5-TWIST1

Plasmid

#215022

Purpose

AAV vector for knocking in an N-terminal tag (FKBP12F36V and V5) for human TWIST1

Depositor

Joanna Wysocka

Article

Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

Insert

twist family bHLH transcription factor 1 (TWIST1 Human)

Use

AAV

Tags

FKBP-V5

Expression

Mutation

Promoter

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_MSX1-FKBP-mNeonGreen-V5

Plasmid

#215025

Purpose

AAV vector for knocking in a C-terminal tag (FKBP12F36V, mNeonGreen, and V5) for human MSX1

Depositor

Joanna Wysocka

Article

Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

Insert

msh homeobox 1 (MSX1 Human)

Use

AAV

Tags

FKBP-mNeonGreen-V5

Expression

Mutation

Promoter

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG_ALX4-Flag-HA

Plasmid

#215028

Purpose

Mammalian expression of human ALX4

Depositor

Joanna Wysocka

Article

Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

Insert

ALX homeobox 4 (ALX4 Human)

Use

Tags

Flag-HA

Expression

Mammalian

Mutation

Promoter

CAG

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pGL4.23_Promoter_5

Plasmid

#210632

Purpose

Luciferase experiment for TAL1 promoter 5

Depositor

Maayan Salton

Article

Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

Insert

promoter 5 (TAL1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Minimal Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pGL4.23_exon 4

Plasmid

#210631

Purpose

Luciferase experiment for TAL1 exon 4

Depositor

Maayan Salton

Article

Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

Insert

TAL1 exon 4 (TAL1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Minimal Promoter

Available since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti_mpx_AsCas12a_h7SK(PacI)_U6(I-SceI)_PGK-puro

Plasmid

#189633

Purpose

Lentiviral expression of dual AsCas12a gRNAs for generating combinatorial AsCas12a 3Cs libraries

Depositor

Manuel Kaulich

Article

Cetin et al Sci Rep. 2023 May 6;13(1):7405. doi: 10.

Insert

h7SK Cas12a gRNA cassette, hU6 Cas12a gRNA cassette, PGK puro cassette

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pSPgRNA_Ex3_1

Plasmid

#210625

Purpose

gRNA target sequences for TAL1 Exon 3

Depositor

Maayan Salton

Article

Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

Insert

TAL1 Exon 3 gRNA (TAL1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pSPgRNA_-60_Enh_2

Plasmid

#210622

Purpose

gRNA target sequences for TAL1 -60 Enhancer

Depositor

Maayan Salton

Article

Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

Insert

TAL1 -60 Enhancer gRNA (TAL1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pSPgRNA_-60_Enh_1

Plasmid

#210621

Purpose

gRNA target sequences for TAL1 -60 Enhancer

Depositor

Maayan Salton

Article

Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

Insert

TAL1 -60 Enhancer gRNA (TAL1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pSPgRNA_-60_Enh_4

Plasmid

#210624

Purpose

gRNA target sequences for TAL1 -60 Enhancer

Depositor

Maayan Salton

Article

Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

Insert

TAL1 -60 Enhancer gRNA (TAL1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pSPgRNA_-60_Enh_3

Plasmid

#210623

Purpose

gRNA target sequences for TAL1 -60 Enhancer

Depositor

Maayan Salton

Article

Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

Insert

TAL1 -60 Enhancer gRNA (TAL1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pSPgRNA_Ex3_2

Plasmid

#210626

Purpose

gRNA target sequences for TAL1 Exon 3

Depositor

Maayan Salton

Article

Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

Insert

TAL1 Exon 3 gRNA (TAL1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pSPgRNA_Ex3_3

Plasmid

#210627

Purpose

gRNA target sequences for TAL1 Exon 3

Depositor

Maayan Salton

Article

Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

Insert

TAL1 Exon 3 gRNA (TAL1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge

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