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Plasmid#113230Purposesafe haven knock-in plasmid for the act5 locus, Expression of H2B-mCherryDepositorInsertH2B
UseSafe haven knock-in plasmid for the act5 locus, h…TagsmCherryExpressionMutationPromoteract5Available sinceAug. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJZC595
Plasmid#62322PurposeExpresses MCP-VP64 and dCas9 in Yeast cellsDepositorInsertsMCP-VP64
dCas9
UseTagsVP64ExpressionYeastMutationHas nuclease-inactivating mutations in the RuvC1 …PromoterTdh3 and pAdhAvailable sinceMarch 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 PDGFRB
Plasmid#67130PurposeStandard for cell-free expression benchmarking.DepositorInsertPDGFRB (PDGFRB Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCA0.002
Plasmid#119560PurposeLevel 0 acceptor vector for promoters with lacZ compatible with blue/white screening, low copy number, pSC101 ori.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCfB6682
Plasmid#106152PurposeEasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6627, integration into Yarrowia lipolytica chromosomal location IntC_2, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
(366) pcDNA3.1(+)-HA-nUbc-(EAAAK)4-OGT(4, K852A)
Plasmid#168189PurposeHA-tagged Ubc6E nanobody fused to an inactive truncated OGT of 4 TPRs with K852A mutationDepositorInsertHA-nUbc6E-(EAAAK)4-OGT(4,K852A)
UseTagsHA-TagExpressionMammalianMutationPromoterT7/CMVAvailable sinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCIneoRL-BCL2 3'UTR
Plasmid#84597PurposeRenilla luciferase reporter containing BCL2 3' UTRDepositorInsertBCL2 3'UTR (UTS2R Human)
UseLuciferaseTagsRenilla LuciferaseExpressionMutationPromoterCMVAvailable sinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-25711gp160
Plasmid#111841PurposeMammalian expression plasmid for Env from the 25711 HIV-1 strainDepositorInsertHIV-1 (25711.2) Env
UseTagsCD5 leader sequenceExpressionMammalianMutationCodon-optimized synthetic genePromoterCMVAvailable sinceMarch 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBDKRB2_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1
Plasmid#101107PurposeEncodes dCas9(C)-VP64 with SAM components fused to BDKRB2DepositorInsertBDKRB2-dCas9(N)
UseCRISPR and Synthetic BiologyTagsFlag and HAExpressionMammalianMutationPromoterAvailable sinceNov. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-sGFP
Plasmid#79508PurposeMultiSite Gateway entry clones, second fragment (attL5, attL2) Super GFP CDSDepositorInsertsuperGFP
UseMultisite gateway entry cloneTags6xHisExpressionMutationPromoterno PromoterAvailable sinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCLXSN(GFP)-myc-BioID-C
Plasmid#174737PurposeOverexpresionDepositorTypeEmpty backboneUseRetroviralTagsExpressionMutationPromoterAvailable sinceSept. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 PRKCA
Plasmid#67115PurposeStandard for cell-free expression benchmarking.DepositorInsertPRKCA Protein kinase C alpha type (PRKCA Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUASTattb-cAMPr Y205A, R626A
Plasmid#84932PurposecAMPr under 5x UAS regulation. pUASTTB for phiC site mediated insertion. Y205A mutation inhibits PKA-C kinase activity. R626A should decrease affinity for cAMP without affecting specifity.DepositorInsertcAMPr Y205A R626A
UseTagsExpressionInsectMutationPKA-C Y205A, PKA-R R626APromoter5X UASAvailable sinceMarch 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A3501
Plasmid#91050PurposeModule A, Promoter: 35S, Gene: dAtCas9-VPR, Terminator: HSPDepositorInsertdAtCas9-VPR
UseCRISPRTagsExpressionMutationPromoter35SAvailable sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPI419
Plasmid#113211Purposesafe haven knock-in plasmid for the act5 locus, N-terminal mScarlet taggingDepositorTypeEmpty backboneUseSafe haven knock-in plasmid for the act5 locus, n…TagsmScarletExpressionMutationPromoterAvailable sinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
Flag Snail 8SA
Plasmid#16222DepositorInsertSnail (SNAI1 Human)
UseTagsFlagExpressionMammalianMutation8SA: S97A, S101A, S105A, S108A, S112A, S116A, S12…PromoterAvailable sinceNov. 30, 2007AvailabilityAcademic Institutions and Nonprofits only -
pUASTattb-cAMPr Y205A, R739A
Plasmid#84933PurposecAMPr under 5x UAS regulation. pUASTTB for phiC site mediated insertion. Y205A mutation inhibits PKA-C kinase activity. R739A should increase affinity for cAMP without affecting specificity.DepositorInsertcAMPr Y205A R739A
UseTagsExpressionInsectMutationPKA-C Y205A, PKA-R R739APromoter5X UASAvailable sinceMarch 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
MSP2266
Plasmid#70705PurposeBacterial expression plasmid for SaCas9 & sgRNA targeted to site 2: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #2DepositorInsertsmammalian codon-optimized Staphylococcus aureus Cas9
SaCas9 sgRNA (+84)
UseCRISPRTagsNLS-3xFLAGExpressionBacterialMutationPromoterT7Available sinceDec. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1(+)-hH2A-(GGS)X6-HaloTag C58
Plasmid#171431PurposeExpression of hH2A-(GGS)X6-HaloTag C58 in mammalian cellsDepositorInserthH2A-(GGS)X6-HaloTag C58
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAVscCBPTuDmiR122 Gpluc
Plasmid#35654DepositorInsertTuD miR-122
UseAAVTagsExpressionMutationPromoterU6Available sinceMay 7, 2012AvailabilityAcademic Institutions and Nonprofits only