Addgene

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Showing: 11381 - 11400 of 55013 results

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attB-mCh-Dnmt3b6-Poly(A)-NeoR

Plasmid

#65554

Purpose

Plasmid for Bxb1-mediated recombination of the mCh-Dnmt3b6 cDNA into a MIN-tagged locus using Neomycin selection

Depositor

Sebastian Bultmann

Article

Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

Insert

Dnmt3b (Dnmt3b Mouse)

Use

Mouse Targeting; Bxb1

Tags

mCherry

Expression

Mammalian

Mutation

isoform 6

Promoter

Available since

Aug. 31, 2015

Availability

Academic Institutions and Nonprofits only

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LoxP3-HA-H2B-Cerulean-2A (SO104)

Plasmid

#99618

Purpose

To clone gene of interest downstream of LoxP3-HA-H2B-Cerulean-2A

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

HA-H2B-Cerulean

Use

Tags

T2A

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC26A9

Plasmid

#132140

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC26A9 (SLC26A9 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 21, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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ISG15 C-term. Domain intein/chitin binding domain (79-156)

Plasmid

#110760

Purpose

Bacterial expression of ISG15 C-term.

Depositor

David Komander

Article

Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

Insert

ISG15 (ISG15 Human)

Use

Tags

intein/chitin binding domain

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA3/FKBP12-pBirA(284-C)-HA

Plasmid

#154452

Purpose

expresses FKBP12-pBirA(284-C)-HA/biochemical assay

Depositor

Hyun-Woo Rhee

Article

Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

Insert

FKBP12-pBirA(284-C)-HA (FKBP12 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

T7 promoter

Available since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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LoxP3-Mb2-HA-mTFP1-2A (SO157)

Plasmid

#99615

Purpose

To clone gene of interest downstream of LoxP3-Mb2-HA-mTFP1-2A

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

Mb2-HA-mTfp1

Use

Tags

T2A

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pYPQ269C2

Plasmid

#164716

Purpose

Gateway entry clone (attL1 & attR5) hAID-CBE_V04 for C-T base editing

Depositor

Yiping Qi

Article

Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

Insert

hAID-zCas9(D10A)-UGI-T2A-MS2-UGI

Use

CRISPR; Gateway compatible haid-cbe_v04 entry clo…

Tags

Expression

Plant

Mutation

Promoter

Available since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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EPHB4-2A-EGFP

Plasmid

#139968

Purpose

Donor vector to knockin EGFP (separated by 2A) into EPHB4 allele

Depositor

James Thomson

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jul 25;11

Insert

Human EPHB4 knockin homology arms (EPHB4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX6p-1-OSF-UFM1

Plasmid

#204565

Purpose

Expresses GST-Strep-FLAG-tagged UFM1 in E.coli

Depositor

Masaaki Komatsu

Article

Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

Insert

UFM1 (UFM1 Human)

Use

Tags

Expression

Bacterial

Mutation

delta84-85aa

Promoter

Available since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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iMb-Control-Mosaic (IR98.10)

Plasmid

#99748

Purpose

Rosa26 gene targeting vector to induce a Cre-dependent mosaic of cells expressing different fluorescent and marker proteins localized to the membrane

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

MbYFP, MbTomato, MbKate2

Use

Cre/Lox and Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pMXs-hs-NRAS

Plasmid

#52728

Purpose

retroviral expression of human NRAS

Depositor

Shinya Yamanaka

Article

Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

Insert

NRAS (NRAS Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 8, 2015

Availability

Academic Institutions and Nonprofits only
tdEos-Mito-7

Plasmid

#57644

Purpose

Localization: Mitochondria, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

Insert

Mito (COX8A Human)

Use

Tags

tdEos

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221_SLC25A10

Plasmid

#132014

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC25A10 (SLC25A10 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 25, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221_SLC25A18

Plasmid

#132003

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC25A18 (SLC25A18 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 24, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pLKO.1-Tet-Puro-shMMERVK10c_1 (inducible)

Plasmid

#189955

Purpose

shRNA mediated knockdown

Depositor

Denes Hnisz

Article

Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

Insert

ERV_MMERVK10c

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC38A10

Plasmid

#132083

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC38A10 (SLC38A10 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 21, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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XLone-Puro Cas13d P2A eGFP-NLS

Plasmid

#155182

Purpose

PiggybacTransposon-based tunable and temporal expression control of Cas13d and nuclear eGFP

Depositor

Xiaoping Bao

Article

Chang et al Biomaterials. 2022 May 6;285:121569. doi

Insert

CasRx

Use

Unspecified; Piggybac transposon

Tags

NLS and P2A eGFP NLS

Expression

Mammalian

Mutation

Promoter

TRE3G

Available since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

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HNF4A_P2-257

Plasmid

#31063

Depositor

Gerhart Ryffel

Article

Thomas et al Hum Mol Genet. 2001 Sep 15. 10(19):2089-

Insert

HNF4A P2 promoter (HNF4A Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

-257 to -1 of P2 HNF4A promoter

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pENTR-2B-Gata4

Plasmid

#98615

Purpose

Gateway entry vector for Gata4

Depositor

Richard Harvey

Article

Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

Insert

Gata4 (Gata4 Mouse)

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

July 26, 2017

Availability

Academic Institutions and Nonprofits only

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HNF4A_P2-285

Plasmid

#31060

Depositor

Gerhart Ryffel

Article

Thomas et al Hum Mol Genet. 2001 Sep 15. 10(19):2089-

Insert

HNF4A P2 promoter (HNF4A Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

-285 to -1 of P2 HNF4A promoter

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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