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Plasmid#195151Purposeimage COR1CDepositorInsertCORO1C (CORO1C Human)
UseTagsExpressionMammalianMutationmutations to make si RNA resistant and remove act…PromoterAvailable sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
SiRES COR1C ACT--Halo
Plasmid#195152Purposeimage COR1CDepositorInsertCORO1C (CORO1C Human)
UseTagsExpressionMammalianMutationmutations to make si RNA resistant and remove act…PromoterAvailable sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
HyWnt3-ΔRep-D3:Luc
Plasmid#192990PurposePlasmid to test the Hydra Wnt3 promoter activity (partial deletion of the repressor element-D3)DepositorInsertHydra Wnt3 promoter
UseTagsExpressionMammalianMutationpartial deletion of the Wnt3 repressor element-D3PromoterAvailable sinceApril 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
HyWnt3-ΔRep-D1:Luc
Plasmid#192988PurposePlasmid to test the Hydra Wnt3 promoter activity (partial deletion of the repressor element-D1)DepositorInsertHydra Wnt3 promoter
UseTagsExpressionMammalianMutationpartial deletion of the Wnt3 repressor element-D1PromoterAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
HyWnt3-ΔRep-D2:Luc
Plasmid#192989PurposePlasmid to test the Hydra Wnt3 promoter activity (partial deletion of the repressor element-D2)DepositorInsertHydra Wnt3 promoter
UseTagsExpressionMammalianMutationpartial deletion of the Wnt3 repressor element-D2PromoterAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
HyWnt3-ΔRep-D4:Luc
Plasmid#192991PurposePlasmid to test the Hydra Wnt3 promoter activity (partial deletion of the repressor element-D4)DepositorInsertHydra Wnt3 promoter
UseTagsExpressionMammalianMutationpartial deletion of the Wnt3 repressor element-D4PromoterAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
HyWnt3-ΔRep:Luc
Plasmid#192987PurposePlasmid to test the Hydra Wnt3 promoter activity (full deletion of the repressor element)DepositorInsertHydra Wnt3 promoter
UseTagsExpressionMammalianMutationRepressor element deletedPromoterAvailable sinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-Swa1::HA-RfA
Plasmid#186404PurposeDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of N-ter HA tag and a gene of interest under control of a reg. sequence in the Swa1 restriction site.DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-Swa1::RfA-ECFP
Plasmid#186402PurposeDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of a gene of interest with a C-ter eCFP under control of a reg. sequence in the Swa1 restriction site.DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-Swa1::venus-RfA
Plasmid#186398PurposeDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of N-ter Venus and a gene of interest under control of a reg. sequence in the Swa1 restriction site.DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-Swa1::RfA-HA
Plasmid#186405PurposeDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of a gene of interest with a C-ter HA tag under control of a reg. sequence in the Swa1 restriction site.DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX459-HypaCas9-CfANLN_sgRNA
Plasmid#183880PurposepX459V2.0-HypaCas9 plasmid with cfANLN sgRNA for N-terminal tagging of anillin in canine (Canis familiaris) cells.DepositorInsertCanis familiaris ANLN sgRNA spacer
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDH_CMV_dimer_mKate2
Plasmid#167333PurposeExpresses mKate2 fused to TFAM no-dimerization mutant in mammalian cellsDepositorInsertTFAM (TFAM Human)
UseLentiviralTagsmKate2ExpressionMammalianMutationChanged K95A, Y99F, E106A, E112A and R116APromoterCMVAvailable sinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDH_CMV_HMGA_mKate2
Plasmid#167334PurposeExpresses mKate2 fused to TFAM HMGA mutant in mammalian cellsDepositorInsertTFAM (TFAM Human)
UseLentiviralTagsmKate2ExpressionMammalianMutationTruncation mutant containing MTS (1-42) and HMGA …PromoterCMVAvailable sinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDH_CMV_L6_mKate2
Plasmid#167332PurposeExpresses mKate2 fused to TFAM L6 mutant in mammalian cellsDepositorInsertTFAM (TFAM Human)
UseLentiviralTagsmKate2ExpressionMammalianMutationChanged K136, H137, K139, R140, K146 and K147 to …PromoterCMVAvailable sinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
Human_TFAM_HMGA_pET28
Plasmid#167326PurposeExpresses TFAM HMGA mutant in bacterial cellsDepositorInsertTFAM (TFAM Human)
UseTags6XHisExpressionBacterialMutationTruncation mutant containing HMGA domain (43-122)PromoterT7Available sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
Human_TFAM_HMGA_linker_pET28
Plasmid#167327PurposeExpresses TFAM HMGA+linker mutant in bacterial cellsDepositorInsertTFAM (TFAM Human)
UseTags6XHisExpressionBacterialMutationTruncation mutant containing HMGA and linker doma…PromoterT7Available sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
Human_TFAM_HMGB_ctail_pET28
Plasmid#167330PurposeExpresses TFAM HMGB+C-tatil mutant in bacterial cellsDepositorInsertTFAM (TFAM Human)
UseTags6XHisExpressionBacterialMutationTruncation mutant containing HMGB and C-tail doma…PromoterT7Available sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
Human_TFAM_dC_pET28
Plasmid#167328PurposeExpresses TFAM dC-tail mutant in bacterial cellsDepositorInsertTFAM (TFAM Human)
UseTags6XHisExpressionBacterialMutationTruncation mutant containing HMGA, linker and HMG…PromoterT7Available sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTrichoGate-17
Plasmid#160717PurposeTrpC Terminator bordered by BsaI restriction sitesDepositorInserttryptophan synthase transcription terminator (TtrpC) bordered by BsaI restriction sites
UseSynthetic BiologyTagsExpressionMutationIncorporation of BsaI restriction sitesPromoterAvailable sinceMarch 3, 2021AvailabilityAcademic Institutions and Nonprofits only