Addgene

We narrowed to 1,544 results for: nif

Showing: 1121 - 1140 of 1544 results

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JAB2519

Plasmid

#190385

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proG1090::BetI-NLS_19St)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proG1090::BetI-NLS_19St

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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JAB2518

Plasmid

#190384

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proG1090::RpaR-NLS_19St)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proG1090::RpaR-NLS_19St

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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JAB2495

Plasmid

#190377

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proG1090::BetI-VP16_19St)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proG1090::BetI-VP16_19St

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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JAB2494

Plasmid

#190376

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proG1090::RpaR-VP16_19St)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proG1090::RpaR-VP16_19St

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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JAB2281

Plasmid

#190362

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proG1090::AmtR-ERF2(AD)-19St)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proG1090::AmtR-ERF2(AD)-19St

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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JAB2180

Plasmid

#190353

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proG1090::TetR-VP16-19St)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proG1090::TetR-VP16-19St

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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JAB2156

Plasmid

#190345

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proG1090::AmtR-VP16-19St)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proG1090::AmtR-VP16-19St

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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L0-I10 PhiC31 GG2-GG4

Plasmid

#195893

Purpose

Level 0 PhiC31 integrase with GG2 GG4 golden gate linkers

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

PhiC31 integrase

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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L0-I11 PhiC31-NLS GG1-GG4

Plasmid

#195894

Purpose

Level 0 PhiC31 inegrase NLS with GG1 GG4 Golden gate linkers

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

PhiC31 integrase

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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L0-I12 PhiC31-NLS GG2-GG4

Plasmid

#195895

Purpose

Level 0 PhiC31 inegrase NLS with GG2 GG4 Golden gate linkers

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

PhiC31 integrase

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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L0-I14 DST GG4-GG5

Plasmid

#195896

Purpose

Level 0 DST (destabilization RNA tag: SAUR tag) with GG4 GG5 golden gate linkers

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

DST

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCRISPR_full-site

Plasmid

#186277

Purpose

Reporter plasmid for full-site selection assay

Depositor

Jennifer Doudna

Article

Wang et al Nat Commun. 2021 May 6;12(1):2571. doi:

Insert

Thiomicrospira leader repeat and modified chloramphenicol selection cassette (removed RBS and start codon))

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pCRISPR

Plasmid

#186276

Purpose

Plasmid containing shortened version of upstream Thiomicrospira CRISPR array for integration assays

Depositor

Jennifer Doudna

Article

Wang et al Nat Commun. 2021 May 6;12(1):2571. doi:

Insert

Thiomicrospira shortened CRISPR array

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pCJH009_proD_Cas12c-sgRNA-GFP_CAM

Plasmid

#183070

Purpose

Plasmid expressing Cas12c sgRNA targeting GFP (1) for in vivo interference assay in bacteria.

Depositor

Jennifer Doudna

Article

Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

Insert

Cas12c single guide RNA targeting GFP

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pSin4 UAP1 F383G

Plasmid

#169891

Purpose

Used for generation of stable mammalian cell lines expressing UAP1 with with F383G mutation

Depositor

Jennifer Kohler

Article

Capota et al Curr Protoc. 2021 Jul;1(7):e201. doi: 10

Insert

UDP-N-acetylhexosamine pyrophosphorylase (UAP1 Human)

Use

Lentiviral

Tags

None

Expression

Mammalian

Mutation

Changed Phenylalanine (F) at position 383 to glyc…

Promoter

Available since

June 17, 2021

Availability

Academic Institutions and Nonprofits only

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pJCC_083 Cas12i1

Plasmid

#171670

Purpose

For bacterial expression of Cas12i1 with an N-terminal His-MBP tag

Depositor

Jennifer Doudna

Article

Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

Insert

Cas12i1

Use

Tags

10X His, TEV protease cleavage site, and maltose-…

Expression

Bacterial

Mutation

Promoter

Available since

June 16, 2021

Availability

Academic Institutions and Nonprofits only

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pLNL1141

Plasmid

#160867

Purpose

PJ23110-RBS-PhCFAT-mRuby2-tL3S2P24_KanR_p15A

Depositor

John Dueber

Article

Latimer et al ACS Synth Biol. 2020 Sep 18. doi: 10.102

Insert

Coniferyl alcohol acyltransferase

Use

Synthetic Biology

Tags

mRuby2

Expression

Bacterial

Mutation

Promoter

PJ23110

Available since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pET19b_4CL:P3S + STS:P4S

Plasmid

#139789

Purpose

expression of biosynthetic enzymes 4CL and STS in fusion with CC

Depositor

Roman Jerala

Article

Fink et al AMB Express. 2020 May 24;10(1):97. doi:

Insert

biosynthetic enzymes 4CL and STS in fusion with coiled-coil segments

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 24, 2020

Availability

Academic Institutions and Nonprofits only

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pAW218

Plasmid

#113240

Purpose

E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader IHF binding site flip

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2
Leader-repeat

Use

Tags

Expression

Bacterial

Mutation

AAAAAATCATTAATTAATAATAGGTTATG->CATAACCTATTATTA…

Promoter

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pUPD-MTS2 (12)

Plasmid

#101698

Purpose

MTS2 in domestication vector

Depositor

Elena Caro Bernat

Article

Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

Insert

CCAT-[MTS from β subunit of Nicotiana plumbaginifolia F1 ATPase]-AATG

Use

Yeast expression

Tags

Expression

Mutation

Promoter

Available since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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