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Plasmid#13749DepositorUseTagsFLAGExpressionMammalianMutationChanged catalytic His 335 to GlyPromoterAvailable sinceFeb. 1, 2007AvailabilityAcademic Institutions and Nonprofits only
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pAdEasy-EGFP-CLASP2 340-1362 9xS/A
Plasmid#24523DepositorInsertCLASP2 (340-1362) 9xS/A (CLASP2 Human)
UseAdenoviralTagsEGFPExpressionMammalianMutationNonphosphorylatable CLASP2 deletion mutant. M…PromoterAvailable sinceJuly 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_altPKA-Cα-mPAGFP
Plasmid#114133PurposeAlternative spliced variant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.DepositorInsertTestis only splice variant of mouse PKA catalytic subunit α that has no myristoylation site, C-terminally tagged by mPAGFP (Prkaca Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pXD70DAmt1-DadR/DadS-Pdadh-DadhABC(R506S, A2)
Plasmid#191631PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), native promoter-dopamine dehydroxylase from dopamine-metabolizing E. lenta A2 strain (R506S mutation) with transcriptional regulators DadR/DadSDepositorInsertDopamine dehydroxylase (R506S mutation, inactive form)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXD71Cas10.0RTcgr-Pct5.1-crRNA(AarI)-RT(Δcgr12)
Plasmid#191651PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(nontargeting spacer) cgr1/cgr2-deleting repair template, used as negative control for pXD71Cas10.4RT4DepositorInsertCymR
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTS2308-Tier1-pGL3-PPGK-NLuc_TB
Plasmid#169551PurposeTier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' insulator sequence derived from the pGL3 plasmid (pGL3-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).DepositorInsertpGL3-insulator-modulated PPGK-driven NanoLuc Luciferase - mTagBFP expression cassette
UseTagsExpressionMammalianMutationPromoterPPGKAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTER SADB shRNA Human
Plasmid#67151PurposeDoxycycline-regulated mammalian expression vector for expressing shRNA against human SADBDepositorInsertBRSK1 (BRSK1 Human)
UseRNAiTagsExpressionMammalianMutationPromoterH1 TetAvailable sinceJuly 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28aAviHisCD72aCTLD
Plasmid#129065Purposegeneration of biotinylated mouse CD72a CTLD protein using bacteriaDepositorInsertCD72a (Cd72 Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pET28aAviHisCD72cCTLD
Plasmid#129066Purposegeneration of biotinylated mouse CD72c CTLD protein using bacteriaDepositorInsertCD72c (Cd72 Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pGL3-SEC61B.N-BSD.P2A.miniIAA7.3xFlag
Plasmid#216250PurposeHDR template to tag endogenous human SEC61B N-terminus with BSD.P2A.miniIAA7.3xFlagDepositorInsertHDR template for human SEC61B (SEC61B HDR template, Human, Synthetic)
UseCRISPR; Hdr templateTagsBSD.P2A.miniIAA7.3xFlagExpressionMammalianMutationPromoterAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSLQ4428 pHR (EF1a-mCherry-P2A-RfxCas13d-2xNLS-ecDHFR-3xFLAG-WPRE)
Plasmid#214885PurposeLentiviral vector encoding TMP-regulatable RfxCas13d-DD fusionDepositorInsertEF1a-mCherry-P2A-RfxCas13d-2xNLS-ecDHFR-3xFLAG-WPRE
UseCRISPR and LentiviralTagsFLAGExpressionMammalianMutationPromoterEF1aAvailable sinceApril 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-FLAG-Akt2-CA
Plasmid#64050PurposeMyristoylated-FLAG-tagged Akt2, constitutively activeDepositorInsertAKT2 (AKT2 Human)
UseLentiviralTagsFLAG and MYRExpressionMammalianMutationPromoterUbiquitinAvailable sinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHub10-His-YFP-PIP2;1
Plasmid#99386Purposeoverexpression and isolation of recombinant At-PIP2;1 proteinDepositorInsertPIP2;1 (PIP2A Mustard Weed)
UseTagsHis and YFPExpressionPlantMutationPromoter2xCaMV35SAvailable sinceAug. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSCV-(pbabe puro mcs)-human CSF-1 full length (CSF4)-IRES-GFP
Plasmid#86796Purposeretroviral expression of human full length CSF-1(256)DepositorInsertCSF-1 (CSF1 Human)
UseRetroviralTagsIRES-GFPExpressionMammalianMutationPromoterAvailable sinceFeb. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mCherry-TSC2(AA,3Q)
Plasmid#192444PurposeEncodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A, K1614Q, K1615Q, R1616Q mutations to disrupt Akt phosphorylation and GAP activity.DepositorInsertmCherry-TSC2(S939A,T1439A,K1614Q,K1615Q,R1616Q ) (TSC2 Human)
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationTSC2 Ser 939 and Thr 1439 (equivalent to 1462 in …PromoterCMVAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR-ZIP(WT)
Plasmid#27134DepositorUseLentiviralTagseGFP and mCherryExpressionMammalianMutationPromoterAvailable sinceApril 12, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-DIO-tdTomato-f
Plasmid#127092PurposeAn AAV genome with tet-inducible, Cre-dependent expression of farnesylated (f) tdTomatoDepositorInserttdTomato-f
UseAAVTagsfarnesylation signal from c-Ha-RasExpressionMammalianMutationPromoterAvailable sinceJune 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T-TRR-C421-Y2404F
Plasmid#182846Purposemutation Y2404F (TAC/TTC) in GST-TRR-C421 (TRR 2011-2431 aa). Expression in E. coli. by IPTG inductionDepositorInsertTRR 2011-2431 (trr Fly)
UseTagsExpressionBacterialMutationmutation Y2404F (TAC/TTC) in GST-TRR-C421 (TRR 20…PromoterAvailable sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
GFP-CLCb (EED/QQN)
Plasmid#47422DepositorInsertCLCb EED/QQN (CLTB Human)
UseTagsEGFPExpressionMammalianMutationEED changed to QQN, in aa20-41 regionPromoterCMVAvailable sinceJuly 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pShuttle-EGFP-CLASP2 340-1362 9xS/A
Plasmid#24373DepositorInsertCLASP2 (340-1362) 9xS/A (CLASP2 Human)
UseTagsEGFPExpressionMammalianMutationNonphosphorylatable CLASP2 deletion mutant. M…PromoterAvailable sinceJune 29, 2010AvailabilityAcademic Institutions and Nonprofits only