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Plasmid#124827PurposeExpresses multimeric human VWF-ECFP fusion protein. The cyan fluorescence protein coding region is inserted between the VWF-D'D3 and A1 domains.DepositorInsertVWF with cyan fluorescent protein fusion between D'D3 and A1 domains (VWF Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSLQ10333
Plasmid#183955PurposeCAG-dCas12a(WT)-HA-miniVPR-mCherry-T2A-HygroRDepositorInsertNuclease-dead Lb Cas12a
UseCRISPRTagsHA, Hygromycin Resistance, and mCherryExpressionMammalianMutationD832APromoterCAGAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGWB545
Plasmid#74879PurposeGateway cloning compatible binary vector for N-terminal fusion with eCFP (CaMV35S promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterCaMV35SAvailable sinceOct. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
Twitch-1 pcDNA3
Plasmid#49812PurposeFluorescent reporter for calcium signalingDepositorInsertTwitch-1
UseTagsECFP and cpCit174ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pNP1 Aqua toehold switch
Plasmid#107356PurposeExpresses Aquamarine (an eCFP derivative) in Ecoli off a T7 promoter when pNP1 Aqua trigger is co-expressedDepositorInserttoehold switch 8 + Aquamarine
UseTagsExpressionBacterialMutationPromoterT7Available sinceJan. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
Accessory plasmid 1
Plasmid#128569Purposefor mixing with SPECS library to produce lentivirusDepositorInsertECFP
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
PU.1-ets-IRES-mCherry
Plasmid#80141Purposeretroviral expression of PU.1-ets (competitive inhibitor of PU.1) and mCherryDepositorInsertPU.1 (Spi1 Mouse)
UseRetroviralTagsIRES-mCherryExpressionMammalianMutationets = PU.1 DNA binding domain (amino acids 159-26…PromoterAvailable sinceJuly 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSLQ10871
Plasmid#183962PurposeU6-SKSKSO(crRNA array)-CAG-dCas12a(WT)-miniVPRDepositorInsertsdCas12a(wildtype)
poly-crRNA array targeting Sox2-Klf4-Oct4
UseCRISPRTagsHAExpressionMammalianMutationD832APromoterCAG and U6Available sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGWB443
Plasmid#74829PurposeGateway cloning compatible binary vector for C-terminal fusion with eCFP (no promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceSept. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSL1180-HR-PUbEGFP-NLS
Plasmid#176681PurposeExpression of nuclear localized EGFP under Ae. aegypti Polyubiquitin promoter (AAEL003888), EGFP version of pSL1180-HR-PUbECFP (Addgene #47917)DepositorInsertEGFP
UseTagsExpressionInsectMutationPromoterAe. aegypti poly-ubiquitin (AAEL003888)Available sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCer-Vamp2(qv/fw)
Plasmid#53238PurposeMutant (Q76V/F77W), tetanus toxin insensitive VAMP2 (synaptobrevin2), N-terminally-tagged with mCerulean, connected by a 4 amino acid linker. For mammalian cells, used with mCit-4aa-SNAP25B for FRET.DepositorInsertVAMP2 (Vamp2 Rat)
UseTags4aa-linker and mCeruleanExpressionMammalianMutationMutated Q76 to V; F77 to W.PromoterAvailable sinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
NLS-GAI(1-92)
Plasmid#37317DepositorInsertGAI (GAI Mustard Weed)
UseTagsNLSExpressionMammalianMutationcontains aa 1-92PromoterCMVAvailable sinceJune 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSWD95Y14D
Plasmid#159098PurposeCckA FRET Sensor with cyclic-di-GMP point mutantDepositorInsertCckA 1-182, Linker SNVTRHRSATE, mClover3, CckA 183-691(Y514D), Linker SNVTRHRSAT, mRuby3
UseTagsExpressionBacterialMutationMutated Y514 to D514PromoterXyloseAvailable sinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSWD95D293A
Plasmid#159099PurposeCckA FRET Sensor with DITE motif mutantDepositorInsertCckA 1-182, Linker SNVTRHRSATE, mClover3, CckA 183-691 (D293A), Linker SNVTRHRSAT, mRuby3
UseTagsExpressionBacterialMutationCckA D293 mutated to A293 and V199I on mRuby3 (P…PromoterXyloseAvailable sinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSLQ10844
Plasmid#183956PurposeCAG-hyperdCas12a-HA-miniVPR-mCherry-T2A-HygroRDepositorInsertHyperdCas12a
UseCRISPRTagsHA, Hygromycin Resistance, and mCherryExpressionMammalianMutationD832A, D156R, E292R, D235R, D350RPromoterCAGAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLPT234
Plasmid#127855PurposeRepressilator 2.0, without degradation tags, integrated triple reporterDepositorInsertsbla
mSCFP3
mKate2
mVenus
TetR
LacI
cI
UseSynthetic BiologyTagsExpressionBacterialMutationFirst 11aa derived from mCherry as fusion proteinPromoterpAmpR, pL LacO1, pL tetO1, and pRAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSLQ10875
Plasmid#183963PurposeCBA-hyperdCas12a-HA-KRAB-mCherryDepositorInsertHyperdCas12a
UseCRISPRTagsHA, KRAB, and mCherryExpressionMammalianMutationD832A, D156R, E292R, D235R, D350RPromoterCBAAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
Twitch-2B pcDNA3
Plasmid#49531PurposeFluorescent reporter for calcium signalingDepositorInsertTwitch-2B
UseTagsECFP and cpCit174ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSLQ10715
Plasmid#183960PurposeU6-crOct4-CAG-hyperdCas12a-miniVPRDepositorInsertsHyperdCas12a
crRNA targeting Oct4
UseCRISPRTagsHAExpressionMammalianMutationD832A, D156R, E292R, D235R, D350RPromoterCAG and U6Available sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only