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Plasmid#57449PurposeLocalization: Actin Capping Protein , Excitation: 507 / 572, Emission: 516 / 580DepositorInsertCCPB1
UseTagsmEos3.2ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 4, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
tdEos-CCPB1-C-18
Plasmid#57591PurposeLocalization: Actin Capping Protein , Excitation: 505 / 569, Emission: 516 / 581DepositorInsertCCPB1
UseTagstdEosExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
mCherry-MCPA2-C-18
Plasmid#55079PurposeLocalization: Actin Capping Protein , Excitation: 587, Emission: 610DepositorInsertMCPA2
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
mGeos2-VESS-Lifeact-7
Plasmid#54526PurposeLocalization: Actin, Excitation: 503 / 569, Emission: 514 / 581DepositorTypeEmpty backboneUseTagsmGeos2-VESS-LifeactExpressionMammalianMutationPromoterAvailable sinceJuly 2, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
mGeos2-VEMS-Lifeact-7
Plasmid#54818PurposeLocalization: Actin, Excitation: 503 / 569, Emission: 514 / 581DepositorTypeEmpty backboneUseTagsmGeos2-VEMS-LifeactExpressionMammalianMutationPromoterAvailable sinceJune 20, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
A6TSR
Plasmid#50359Purposeexpresses T.gondii MIC2(67-660) domains in mammalian cellsDepositorInsertMIC2-A6TSR
UseTagsHISExpressionMammalianMutationS158A, N357S, N463S, N470DPromoterCHICK beta-actin promoterAvailable sinceMarch 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
PH-Btk-mRuby2
Plasmid#124657PurposePI(3,4,5)P3 probe. PH domain of BTK fused with mRuby2DepositorInsertBTK (BTK Human)
UseTagsmRuby2ExpressionMammalianMutationcontains only the PH domain of Bruton tyrosine ki…PromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7-EF-EYFP-YAP1_S94A-PolyA
Plasmid#112286PurposeFluorescently labels human YAP1-2gamma isoform (504 aa) S94A mutantDepositorInsertEYFP-YAP1 (YAP1 Human)
UseLentiviralTagsExpressionMutationHuman YAP1-2gamma with Serine 94 to AlaninePromoterEF-1 alphaAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-DeAct-GS1
Plasmid#89445PurposeExpresses EGFP-GS1 (Human Gelsolin 53-176) in mammalian cellsDepositorInsertDeAct-GS1 (GSN Human)
UseTagsEGFPExpressionMammalianMutationTruncation spanning cytoplasmic Gsn amino acids 5…PromoterCMVAvailable sinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSico-CAG-v-H-Ras-IRES-Luciferase/EGFP
Plasmid#58959Purposelentiviral expression of v-H-Ras and luciferase/EGFP reporterDepositorInsertsCMV enhancer/chicken beta actin promoter
v-H-Ras
luc2/EGFP fusion gene
UseLentiviralTagsExpressionMammalianMutationPromoterCMV enhancer/chicken beta actinAvailable sinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLL5.0 E-cadherin shRNA/E-cadherin GFP-IRES-HA-H-Ras V12
Plasmid#101285PurposeLentiviral expression ,was generatedby recombining bothIRES an HA-tagged H-Ras V12 into SbfI site of pLL5.0 Ecadherin shRNA/mEcad-GFPDepositorInsertE-Cadherin-GFP-H-Ras
UseLentiviralTagsGFPExpressionMammalianMutationH-Ras V12PromoterAvailable sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTetON-DHFRdd-SpvB; CMV-mCherry
Plasmid#89463PurposeTet/Dox-inducible expression of DHRFdd-SpvB (Salmonella SpvB 375-591) plus constitutive expression of mCherry in mammalian cells (Dual-promoter)DepositorInsertDeAct-SpvB (spvB Salmonella enterica)
UseTagsE. coli DHFRdd destabilization domain and mCherry…ExpressionMammalianMutationTruncation spanning SpvB amino acids 375-591 (mon…PromoterTRE3GAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2-sgNCKAP1-2
Plasmid#210134Purposeknock out NCKAP1 in mammalian cellsDepositorInsertNck-associated protein 1 (NCKAP1 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2-sgNCKAP1-1
Plasmid#210133Purposeknock out NCKAP1 in mammalian cellsDepositorInsertNck-associated protein 1 (NCKAP1 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTetON-DeAct-GS1
Plasmid#89454PurposeTet/Dox-inducible expression of EGFP-GS1 (Human Gelsolin 53-176) in mammalian cellsDepositorInsertDeAct-GS1 (GSN Human)
UseTagsEGFPExpressionMammalianMutationTruncation spanning cytoplasmic Gsn amino acids 5…PromoterTRE3GAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP C1-Eps8 WT
Plasmid#74950Purposemammalian expression of Eps8 fused to GFPDepositorInsertEps8 (Eps8 Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceMay 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
aL-EGFP
Plasmid#103879Purposefull-length human aL with EGFP at betapropellerDepositorInsertfull-length human aL with EGFP in betapropeller (ITGAL Human)
UseTagstension sensor moduleExpressionMutationPromoterAvailable sinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSNAP-CAPZ
Plasmid#69948PurposeSimultaneous expression of a His- and SNAP-tagged β1 subunit and untagged α1 subunits of chicken CapZDepositorUseTagsHis 6 and SNAPExpressionBacterialMutationPromoterAvailable sinceNov. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV-MRLC1-mRuby2-IRES-PuroR
Plasmid#103031PurposeLentiviral expression of mRuby2-tagged MRLC with puromycin selectionDepositorInsertMRLC1 (MYL9 Human)
UseLentiviralTagsmRuby2ExpressionMutationPromoterCMV immediate earlyAvailable sinceMay 22, 2018AvailabilityAcademic Institutions and Nonprofits only -