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Plasmid#67658Purposecyan luminescent protein for high-speed single-cell and whole body imagingDepositorInsertCyan Nano-lantern
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBad-CA TAG20
Plasmid#105666Purposeexpresses thermostable variant of human carbonic anhydrase II with TAG stop codon at site 20 and C-terminal 6x his tagDepositorArticleInserthuman carbonic anhydrase II thermostable variant (CA2 Human)
UseTags6x his tagExpressionBacterialMutationTAG stop codon at site 20PromoterpBad/arabinoseAvailable sinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
Cyan Nano-lantern(Ca2+)/pcDNA3
Plasmid#71344PurposeExpress Cyan Nano-lantern-based luminescent Ca2+ indicator in mammalian cellDepositorInsertCyan Nano-lantern(Ca2+)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMG343
Plasmid#176183Purposepreassembled yeast MoClo level-1 backbone with toxic gene dropout cassette (ccdB and TPK2) and URA3 markerDepositorInsertTPK2+ccdB dropout cassette
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKan-PCUP1-AID*(N)
Plasmid#99525PurposeN-terminal AID* degron cassette with CUP1 promoter and KanR marker for S. cerevisiaeDepositorInsertIAA17(71-117) (AXR3 Mustard Weed)
UsePcr template for yeast tagging cassetteTagsExpressionMutationPromoterCUP1Available sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBad-CA TAG93
Plasmid#105667Purposeexpresses thermostable variant of human carbonic anhydrase II with TAG stop codon at site 93 and C-terminal 6x his tagDepositorArticleInserthuman carbonic anhydrase II thermostable variant (CA2 Human)
UseTags6x his tagExpressionBacterialMutationTAG stop codon at site 93PromoterpBad/arabinoseAvailable sinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCS2-zfKitlgb
Plasmid#168200PurposeExpress zebrafish Kitlgb, mRNA synthesisDepositorInsertKitlgb (kitlgb Zebrafish)
UseVertebrate expression (zebrafish, chicken, xenopu…TagsExpressionMammalianMutationI85V, V222I, and D233N- please see depositor comm…PromoterAvailable sinceNov. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBEAST-J23101-CocE
Plasmid#114600Purpose"Strong constitutive expression of metabolic enzyme, CocE, for cell-free expression"DepositorInsertCocE
UseSynthetic Biology; Cell-free protein synthesisTagsExpressionMutationPromoterJ23101Available sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHyg-AID(1-114)-8myc
Plasmid#99516PurposeTruncated C-terminal AID-8myc degron cassette with HygR marker for S. cerevisiaeDepositorInsertIAA17(1-116) (AXR3 Mustard Weed)
UsePcr template for yeast tagging cassetteTags8xMycExpressionMutationPromoterAvailable sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
Orange Nano-lantern(Ca2+)/pcDNA3
Plasmid#71346PurposeExpress Orange Nano-lantern-based luminescent Ca2+ indicator in mammalian cellDepositorInsertOrange Nano-lantern(Ca2+)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-R2ACR_142-EYFP
Plasmid#103964PurposeHighly efficient anion channelrhodopsin from Rhodomonas sp. (CCAC1630) for expression in mammalian cellsDepositorInsertAnion channelrhodopsin R2ACR_142
UseTagsEYFPExpressionMammalianMutationhuman codon optimizedPromoterCMVAvailable sinceNov. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pIRTetR-GAL80
Plasmid#185853PurposePlugging the mechanism of tetracycline-mediated repression of GAL promoters in Saccharomyces cerevisiaeDepositorInsertgal80Arm1-PAgTEF1-KanMX4-TAgTEF1-PHAC1>TetR>TUP1>PABF1- PTEF1+[TetO]>GAL80Arm2
UseTagsExpressionYeastMutationNonePromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Tight-Puro_Myt1
Plasmid#64845PurposeA lentiviral vector for the expression of mouse Myt1 under doxycyline controlDepositorInsertMyt1 (Myt1 Mouse)
UseLentiviralTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceMay 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJEC626
Plasmid#174367PurposeActivation domain for modular CRISPRa. AD-SYNZIP. Expresses alphaNTD from the E coli RNAP with a SYNZIP17 domain fused to the N terminus.DepositorInsertAlpha N-terminal domain from the E. coli RNA polymerase fused to SYNZIP17
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23106Available sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCNL-N1_zyxin
Plasmid#65682PurposeExpresses Zyxin-CNL in mammalian cellsDepositorInsertzyxin (Zyx Mouse)
UseLuciferaseTagsCNLExpressionMammalianMutationPromoterCMVAvailable sinceJune 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pICSL11023
Plasmid#117542PurposeLevel 1 Golden Gate Cassette: Cas9 expression cassette for dicotyledonous plantsDepositorInsert2x35S+5'UTR OMEGA (pICH51288) + SpCas9-h (no stop codon)(pICSL90005) + YFP (pICSL50005)+ Nos (pICH41421)
UseTagsExpressionPlantMutationPromoter2x35S_OMEGA(pICH51288)Available sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCNL-N1_Mito
Plasmid#65670PurposeExpresses Mito-CNL in mammalian cellsDepositorInsertcytochrome c oxidase subunit VIII
UseLuciferaseTagsCNLExpressionMammalianMutationPromoterCMVAvailable sinceJune 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGAL4-DBD-RUNX2wt-IDR
Plasmid#145252PurposeExpresses fusion of GAL4 DNA-binding domain and RUNX2Awt IDRDepositorInsertRUNX2Awt-IDR
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEPOR1CB0112
Plasmid#117548PurposeLevel 1 Golden Gate Cassette: xCas9 3.7 expression cassette for dicotyledonous plantsDepositorInsert2x35S+5'UTR OMEGA (pICH51288) + Sp-xCas9 3.7 (no stop codon) (pEPOR0SP0011) + YFP (pICSL50005) + Nos (pICH41421)
UseTagsExpressionPlantMutationPromoter2x35S+5'UTR OMEGA (pICH51288)Available sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pONL-N1_Golgi
Plasmid#65695PurposeExpresses Golgi-ONL in mammalian cellsDepositorInsertN-terminal 81 amino acids of beta 1,4-galactosyltransferase
UseLuciferaseTagsONLExpressionMammalianMutationPromoterCMVAvailable sinceJune 17, 2015AvailabilityAcademic Institutions and Nonprofits only