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Plasmid#198728PurposeExpresses two gRNAs targeting Arabidopsis NIA1 gene for knockout. Neither gRNA is fused to a mobility signal and are therefore non-mobile.DepositorInsertAtNIA1 gRNAs
UseTagsExpressionPlantMutationPromoterpU6-26, pU6-29Available sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKL001
Plasmid#98917PurposeConstitutively expresses the CaPR construct, the voltage indicator PROPS tethered to the calcium sensor GCaMP6f, under the Sigma 70 promoter in E. coli.DepositorInsertCaPR
UseTagsExpressionBacterialMutationPromoterBBa_J23118 - Strong ConstitutiveAvailable sinceAug. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
PB-CT3G-ERP2-MG-IRES2-mNeonGreen
Plasmid#175506PurposeAll-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mNeonGreen (hEF1a-driven rtTA and puromycin resistance)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE3G-mCMVAvailable sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTSSX-STC
Plasmid#115668PurposeE.coli expression plasmid of small tetraheme cytochrome (STC) from Shewanella oneidensis MR-1 with cleavable Strep tagDepositorInsertsmall tetraheme cytochrome c
UseTagsSignal peptide (aa1-27)-Strep-II tag- Factor Xa c…ExpressionBacterialMutationPromotertacAvailable sinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+) ZKSCAN1 MCS-WT Split GFP + Antisense IRES
Plasmid#69910PurposeExpresses a GFP circular RNA containing the antisense EMCV IRES in mammalian cellsDepositorInsertZKSCAN1, GFP (ZKSCAN1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCR1063
Plasmid#111091PurposeExpresses dCas9-KRAB tagged with mCherry: pHR-hEF1a-dCas9-mcherry-KRAB-mPGK-HygDepositorInsertdCas9-KRAB
UseLentiviralTagsmCherryExpressionMutationPromoterAvailable sinceOct. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT Laccase2 MCS-WT Split GFP
Plasmid#69891PurposeExpresses a GFP circular RNA in DrosophilaDepositorInsertGFP, laccase2 (stw Fly)
UseTagsExpressionInsectMutationPromoterMetallothionein Promoter (pMT)Available sinceNov. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSUPER.retro.puro-HCF-1-D
Plasmid#36997DepositorInsertHCF-1 shRNA (HCFC1 Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSUPER.retro.puro-HCF-1-B
Plasmid#36996DepositorInsertHCF-1 shRNA (HCFC1 Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+) ZKSCAN1 MCS-ZKSCAN1 548-1417 (No intron)
Plasmid#69904PurposeExpresses a miniature version of the ZKSCAN1 circular RNA in mammalian cellsDepositorInsertZKSCAN1 (ZKSCAN1 Human)
UseTagsExpressionMammalianMutationF169L in ZKSCAN1PromoterCMVAvailable sinceNov. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT Laccase2 MCS Exon Vector
Plasmid#69884PurposeExpression plasmid for expressing circular RNAs of a desired sequence in DrosophilaDepositorInsertLaccase2 (stw Fly)
UseTagsExpressionInsectMutationPromoterMetallothionein Promoter (pMT)Available sinceOct. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBAD02_CBASS_Vibrio_vulnificus
Plasmid#224405PurposeFor expression of the type I CBASS system encoded by Vibrio vulnificusDepositorInsertEndonuclease_Phospholipase_VvCdnG_AGS-C
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBAD02_CBASS_Vibrio_campbellii
Plasmid#224404PurposeFor expression of the type I CBASS system encoded by Vibrio campbelliiDepositorInsertEndonuclease_VcCdnG_AGS-C
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBAD02_CBASS_Pseudoalteromonas_sp._A2
Plasmid#224401PurposeFor expression of the type I CBASS system encoded by Pseudoalteromonas sp. A2DepositorInsertPsp.CdnB_AGS-C_Cap15
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBAD02_CBASS_Pectobacterium_parmentieri
Plasmid#224400PurposeFor expression of the type I CBASS system encoded by Pectobacterium parmentieriDepositorInsertPpCdnB_AGS-C_Cap15
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBAD02_CBASS_Alteromonas_sp._ALT199
Plasmid#224399PurposeFor expression of the type I CBASS system encoded by Alteromonas sp. ALT199DepositorInsertAsp.CdnB_AGS-C_Cap15
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBAD02_CBASS_Rhodanobacter_denitrificans
Plasmid#224391PurposeFor expression of the type I CBASS system encoded by Rhodanobacter denitrificansDepositorInsertPhospholipase_RdCdnA
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSF1b-His8-TEV-PI31-1-151
Plasmid#202593PurposeBacterial expression of HsPI31-1-151DepositorInsertPI31 (PSMF1 Human)
UseTags8xHis, TEVExpressionBacterialMutationaa 1-151 onlyPromoterAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
FoxFTVC+bpFOG_MEK-S216D, S220E
Plasmid#107522PurposeConstitutive activation of FGF/MAPK pathway by MEK-S216D, S220E mutantDepositorInsertMEK
UseTagsExpressionMutationchanged Serine 216 to Aspartic Acid and Serine 22…PromoterTVC-specific FoxF enhancer (FoxFTVC+bpFOG)Available sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
sUPRa
Plasmid#223242PurposesUPRa is a dual promoter and two-color construct, which is designed to report the global unfolded protein response (UPR) and affords unbiased cell detection irrespective of UPR levelsDepositorInsertsUPRa
UseAAVTagsExpressionMutationPromoterCustomAvailable sinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only