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Showing: 1121 - 1140 of 1258 results

  1. Desmoplakin I donor only control (F40-based tension sensor)

    Plasmid
    #119188
    Purpose
    The donor (mTFP1) only control for the F40-based human desmoplakin I tension sensor can be used for bleedthrough calibration and provides the donor only lifetime to determine FRET efficiency.
    Depositor
    Alex Dunn
    Article
    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:
    Insert
    human Desmoplakin I-[mTFP1-F40-mEYFP(Y67G)] (internal-1945) (DSP Human)
    Use
    Transposon
    Tags
    mTFP1-F40-mEYFP
    Expression
    Mammalian
    Mutation
    inserted F40-based tension sensor module after aa…
    Promoter
    TCE
    Available since
    Dec. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    119188-plasmid-map-sequence-id-230532

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  6. pMVP and pMAGIC Cloning Systems

    Plasmid Kit
    #1000000155
    Purpose
    pMVP and pMAGIC are cloning systems for adenoviral, lentiviral, expression, PiggyBac transposon, and Sleeping Beauty transposon vectors for transgene or RNAi delivery and dCas9-based engineering.
    Depositor
    Christopher Newgard
    Available since
    Sept. 6, 2019
    Availability
    Academic Institutions and Nonprofits only

  7. pJMH058

    Plasmid
    #186640
    Purpose
    aadA.co-TaadA-PcpcB-eYFP.co between homology arms for pilA
    Depositor
    Adam Arkin
    Article
    Jacob M. et al bioRxiv
    Insert
    aadA.co-TaadA-PcpcB-eYFP.co
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    186640-plasmid-map-sequence-id-377053

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  8. pJMH059

    Plasmid
    #186641
    Purpose
    aadA.co-TaadA-PrbcL-eYFP.co between homology arms for pilA
    Depositor
    Adam Arkin
    Article
    Jacob M. et al bioRxiv
    Insert
    aadA.co-TaadA-PrbcL-eYFP.co
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    186641-plasmid-map-sequence-id-381469

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  9. pJMH139

    Plasmid
    #186642
    Purpose
    aadA.co-TaadA-PJ23119-eYFP.co between homology arms for pilA
    Depositor
    Adam Arkin
    Article
    Jacob M. et al bioRxiv
    Insert
    aadA.co-TaadA-PJ23119-eYFP.co
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    186642-plasmid-map-sequence-id-381468

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  10. pJMH140

    Plasmid
    #186643
    Purpose
    aadA.co-TaadA-PJ23111-eYFP.co between homology arms for pilA
    Depositor
    Adam Arkin
    Article
    Jacob M. et al bioRxiv
    Insert
    aadA.co-TaadA-PJ23111-eYFP.co
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    186643-plasmid-map-sequence-id-377051

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  11. pJMH141

    Plasmid
    #186644
    Purpose
    aadA.co-TaadA-PJ23100-eYFP.co between homology arms for pilA
    Depositor
    Adam Arkin
    Article
    Jacob M. et al bioRxiv
    Insert
    aadA.co-TaadA-PJ23100-eYFP.co
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    186644-plasmid-map-sequence-id-377050

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  12. pJMH142

    Plasmid
    #186645
    Purpose
    aadA.co-TaadA-PJ23107-eYFP.co between homology arms for pilA
    Depositor
    Adam Arkin
    Article
    Jacob M. et al bioRxiv
    Insert
    aadA.co-TaadA-PJ23107-eYFP.co
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    186645-plasmid-map-sequence-id-377049

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  13. pJMH143

    Plasmid
    #186646
    Purpose
    aadA.co-TaadA-PJ23117-eYFP.co between homology arms for pilA
    Depositor
    Adam Arkin
    Article
    Jacob M. et al bioRxiv
    Insert
    aadA.co-TaadA-PJ23117-eYFP.co
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    186646-plasmid-map-sequence-id-377048

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  16. Desmoplakin I affinity mutant (F40-based tension sensor)

    Plasmid
    #119190
    Purpose
    The affinity mutant for the F40-based human desmoplakin I tension sensor incorporates a point mutation that exhibits enhanced keratin association.
    Depositor
    Alex Dunn
    Article
    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:
    Insert
    human Desmoplakin I(S2849G)-[mTFP1-F40-mEYFP] (internal-1945) (DSP Human)
    Use
    Transposon
    Tags
    mTFP1-F40-mEYFP
    Expression
    Mammalian
    Mutation
    inserted F40-based tension sensor module after aa…
    Promoter
    TCE
    Available since
    Jan. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    119190-plasmid-map-sequence-id-232293

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  17. pAAV-Ef1a-DIO-PPO-Venus

    Plasmid
    #139505
    Purpose
    AAV vector for Cre-dependent expression of Venus-tagged parapinopsin driven by the Ef1a promoter. Used for photoswitchable control of inhibitory GPCR signaling cascades.
    Depositor
    Michael Bruchas
    Article
    Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11
    Has Service
    AAV8 and AAV9
    Insert
    parapinopsin
    Use
    AAV
    Tags
    Venus tag following 3x Ala linker
    Expression
    Mammalian
    Mutation
    Promoter
    Ef1a
    Available since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    139505-plasmid-map-sequence-id-276175

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  19. pAAV-CAG-DIO-PPO-Venus

    Plasmid
    #139504
    Purpose
    AAV vector for Cre-dependent expression of Venus-tagged parapinopsin driven by the CAG promoter. Used for photoswitchable control of inhibitory GPCR signaling cascades.
    Depositor
    Michael Bruchas
    Article
    Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11
    Has Service
    AAV5
    Insert
    parapinopsin
    Use
    AAV
    Tags
    Venus tag following 3x Ala linker
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    139504-plasmid-map-sequence-id-276180

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  20. G protein alpha-q-CFP

    Plasmid
    #66081
    Purpose
    This G protein alpha-q construct contains internal insertions of CFP and the EE epitope
    Depositor
    Catherine Berlot
    Article
    Hughes et al J Biol Chem. 2001 Feb 9;276(6):4227-35.
    Insert
    G protein alpha-q-EE-CFP (Gnaq Mouse)
    Use
    Tags
    CFP was inserted internally into the alpha-q sequ…
    Expression
    Mammalian
    Mutation
    BamHI and SacI sites in alpha-q were removed with…
    Promoter
    CMV
    Available since
    July 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    66081-plasmid-map-sequence-id-118921

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Showing: 1121 - 1140 of 1258 results