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Plasmid#155156PurposeExpresses (Strep-MlaF_K47A)-(6xHis-TEV-MlaB)DepositorInsertMlaF-(6xHis-TEV-MlaB)
UseTagsExpressionMutationMlaF_K47APromoterT7 promoterAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBEL2077
Plasmid#155157PurposeExpresses (Strep-MlaF_E170Q)-(6xHis-TEV-MlaB)DepositorInsertMlaF-(6xHis-TEV-MlaB)
UseTagsExpressionMutationMlaF_E170QPromoterT7 promoterAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBEL2078
Plasmid#155158PurposeExpresses (Strep-MlaF_Y261A)-(6xHis-TEV-MlaB)DepositorInsertMlaF-(6xHis-TEV-MlaB)
UseTagsExpressionMutationMlaF_Y261APromoterAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBEL2079
Plasmid#155159PurposeExpresses (Strep-MlaF_L265A)-(6xHis-TEV-MlaB)DepositorInsertMlaF-(6xHis-TEV-MlaB)
UseTagsExpressionMutationMlaF_L265APromoterAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBEL1244
Plasmid#155138PurposeExpresses MlaFED operon in E. coliDepositorInsertmlaFE-[6xHis-2xQH-TEV-mlaD]-mlaC operon
UseTagsExpressionMutationPromoteraraBADAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
plIVMD118
Plasmid#160938PurposeExcess Capsid Protein in vivo mRNA displayDepositorInsertMCP-mCherry-FLAG tag
UseTagsMYC tagExpressionYeastMutationPromoterMET25Available sinceNov. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
plIVMD115
Plasmid#160937PurposeExcess Capsid Protein for in vivo mRNA displayDepositorInsertMCP-mCherry-MYC tag
UseTagsMYC tagExpressionYeastMutationPromoterMET25Available sinceNov. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
EcNusGNTD
Plasmid#113123PurposeExpresses N-terminus of E. coli NusGDepositorInsertNusG
UseTagsHis6-MBP-N10-TEVExpressionBacterialMutationDeleted residues 120-181PromoterT7Available sinceOct. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKM45
Plasmid#154152PurposeFirefly luciferase with no promoter for negative control for luciferase assaysDepositorInsertFirefly luciferase
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQP-iRISB
Plasmid#138979PurposeLight-controlled tri-directional targeting of endogenous proteinsDepositorInsertNcoI-BphP1-CAAX-IRES2-NES-SGlinkeriB( GFP)-mCherry-Q-PAS1-AsLOV2cNLS-NotI
UseTagsExpressionMutationPromoterAvailable sinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQP-2363
Plasmid#138975PurposeRelocalization of genomically expressed protein of interest from cytoplasm to nucleus upon near-infrared illuminationDepositorInsertNcoI-BphP1- iB(GFP) -IRES2-NES-mCherry-QPAS1- NLS-NotI
UseTagsExpressionMutationPromoterAvailable sinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS416-PTEF1-MCP-3P-ECM33_intron
Plasmid#127601Purposelow-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter, 3P-ECM33 intron inserted into URA3DepositorInsertECM33 intron with MS2 hairpins inserted near 3′ end of intron
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS416-PTEF1-MCP-5P-ECM33_intron
Plasmid#127599Purposelow-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter, 5P-ECM33 intron inserted into URA3DepositorInsertECM33 intron with MS2 hairpins inserted near 5′ end of intron
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS416-PTEF1-MCP-ECM33_intron
Plasmid#127598Purposelow-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter, ECM33 intron inserted into URA3DepositorInsertECM33 intron
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
p5343_UC57
Plasmid#126645Purposeenvironmental plasmid p5343 cloned into pUC57DepositorInsertEnvironmental plasmid p5343
UseSynthetic BiologyTagsExpressionMutationPromoterNAAvailable sinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTK_Zfhx4_enh_12_Citrine
Plasmid#130597Purposeenhancer reporterDepositorInsertZfhx4_enh_12
UseEnhancer reporterTagsExpressionMutationPromoterthymidine kinase minimal promoterAvailable sinceNov. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTK_Zfhx4_enh_316_Cerulean
Plasmid#130598Purposeenhancer reporterDepositorInsertZfhx4_enh_316
UseEnhancer reporterTagsExpressionMutationPromoterthymidine kinase minimal promoterAvailable sinceNov. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTK_Lmo4_enh_15_Citrine
Plasmid#130599Purposeenhancer reporterDepositorInsertLmo4_enh_15
UseEnhancer reporterTagsExpressionMutationPromoterthymidine kinase minimal promoterAvailable sinceNov. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTK_Lmo4_enh_17_Citrine
Plasmid#130601Purposeenhancer reporterDepositorInsertLmo4_enh_17
UseEnhancer reporterTagsExpressionMutationPromoterthymidine kinase minimal promoterAvailable sinceNov. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTK_Brn3_enh_250_Citrine
Plasmid#130612Purposeenhancer reporterDepositorInsertBrn3_enh_250
UseEnhancer reporterTagsExpressionMutationPromoterthymidine kinase minimal promoterAvailable sinceNov. 12, 2019AvailabilityAcademic Institutions and Nonprofits only