-
Plasmid#118752PurposeMultisite gateway destination vector for 3' tagging with EGFP for cell free expression. Parton lab FEXDepositorInsertccdb
UseTagsEGFPExpressionMutationPromoterAvailable sinceSept. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
SITS-mCherry-ccdb
Plasmid#118629PurposeMultisite gateway destination vector for 5' tagging with mCherry for cell free expression. Parton lab clone FKZDepositorInsertccdb
UseTagsmCherryExpressionMutationPromoterAvailable sinceSept. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
SITS-EGFP-ccdb
Plasmid#118628PurposeMultisite gateway destination vector for 5' tagging with EGFP for cell free expression. Parton lab clone FEZDepositorInsertccdb
UseTagsEGFPExpressionMutationPromoterAvailable sinceSept. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet_DL121_WTTS
Plasmid#171954PurposeSelection vector for chimeric fusion of ecDHFR and asLOV2.DepositorInsertsFusion of ecDHFR and asLOV2 at position 121 of DHFR
Thymidylate Synthase
UseTags8-His tagExpressionBacterialMutationPromoterT7Available sinceAug. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBD21_035
Plasmid#109152PurposepL2 plasmid backbone for assembly of a transcription unit along with constitutive expression of EGFPDepositorInsertsmRFP
EGFP
UseSynthetic BiologyTagsExpressionBacterial and MammalianMutationPromoterCMV and pTetAvailable sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTJV1ScKm-rpoB
Plasmid#166980PurposeGenerates ssDNA in vivo targeting E. coli rpoB for recombineering w/ Beta recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; npt2 for kanamycin resistanceDepositorInsertNeoR/KanR
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pdCas9_CL
Plasmid#166245Purposeregulated dCas9 generatorDepositorInsertP112-sgRNA-term-P104-RBS1-dCas9-B15
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterEc-TTL-P112 and Ec-TTL-P104Available sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAUX_OL
Plasmid#166246Purposeuse as an auxiliary temperature-sensitive plasmid to successfully transform pdCas9_CL plasmidDepositorInsert[P112-sgRNA-term]-[J23116_B34-dCas9-B15]
UseTagsExpressionBacterialMutationPromoterEc-TTL-P112 and BBa_J23116Available sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCTCON2-GXB
Plasmid#160926PurposeDual fluorescent protein reporter (GFP-BFP) for ncAA incorporation (TAG construct)DepositorInsertsBFP
sfGFP
UseTagsExpressionYeastMutationPromoterGAL1-10Available sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFapO_94
Plasmid#149597PurposepXI-3-LoxP-KlURA3-pTEF1- FapO_94-yeGFPDepositorInsertyeGFP
UseSynthetic BiologyTagsExpressionYeastMutationPromoterTEF1-FapO_94Available sinceDec. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pXylO_94
Plasmid#149598PurposepXI - 3 -LoxP -KlURA3 -pTEF1 - XylO_94 -yeGFPDepositorInsertyeGFP
UseSynthetic BiologyTagsExpressionYeastMutationPromoterTEF1-XylO_94Available sinceDec. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTetO_105
Plasmid#149599PurposepXI - 3 -LoxP -KlURA3 -pTEF1 - TetO_105 -yeGFPDepositorInsertyeGFP
UseSynthetic BiologyTagsExpressionYeastMutationPromoterTEF1-TetO_105Available sinceDec. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFA271
Plasmid#149600Purpose(parent plasmid pCFB262) pXII - 4 -LoxP -SpHIS5 -209bp - pCYC1_BenO_183 -yeGFPDepositorInsertyeGFP
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceDec. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFA272
Plasmid#149601Purpose(parent plasmid pCFB262) pXII - 4 -LoxP -SpHIS5 -209bp - pCYC1_BenO_182 -yeGFPDepositorInsertyeGFP
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceDec. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFA197
Plasmid#149602Purpose(parent plasmid pCFB262) pXII - 4 -LoxP -SpHIS5 -209bp - pCYC1 -PcaO -108 -yeGFPDepositorInsertyeGFP
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPcaO_1O8Available sinceDec. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVD_79
Plasmid#149603Purpose(parent plasmid pCFB257) pX - 3 -LoxP -KlLEU2 -pTEF1 - TetRDepositorInsertTetR
UseSynthetic BiologyTagsExpressionYeastMutationPromoterTEF1Available sinceDec. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEE22
Plasmid#161787PurposeHi-SC2 reporter delivery plasmidDepositorInsertHi-SC2 reporter
UseConjugative plasmid to deliver hi-sc2 reporterTagsExpressionMutationHimar site included MmeI site, tandem loxP sites,…Promotern/aAvailable sinceDec. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCL66-decoder
Plasmid#159453PurposeInsert: Pfus1mut-tetR-nls-malE-tCyc1. Same insert as pCL33-decoder in a multiple integration shuttle vector. Backbone: pRG235 (addgene). Marker: Leu2.DepositorInsertPfus1mut-tetR-nls-malE-tCyc1
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPfus1mutAvailable sinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVD_01
Plasmid#149606PurposepTEF1-VanR inserted in pRS416 backboneDepositorInsertVanR
UseSynthetic BiologyTagsExpressionYeastMutationPromoterTEF1Available sinceDec. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
907F1
Plasmid#160233PurposeFlightN-VanR-VP16-3X-SV40-attBDepositorInsertsVanR
Flightin promoter
UseTagsVP16ExpressionInsectMutationPromoterAvailable sinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only